LOCUS       sequence-479335-        4350 bp    DNA     circular SYN 27-JUL-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC34A3_STOP
FEATURES             Location/Qualifiers
     source          1..4350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(580..607)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(699..785)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     834..856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     848..865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     849..865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    881..980
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2787..2886)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2903..2922)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2904..2922)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2927..2943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2927..2943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3045..3064)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3056..3865
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3123..3142)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(4012..4350,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      481 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      541 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
      601 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
      661 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
      721 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
      781 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
      841 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga ttttattttg
      901 actgatagtg acctgttcgt tgcaacacat tgatgagcaa tgctttttta taatgccaac
      961 tttgtacaaa aaagcaggct ccaccatgcc aagctccctg ccaggaagcc aggtgccaca
     1021 ccccaccctg gacgccgtgg atctggtgga gaagaccctg agaaacgagg gcacatctag
     1081 ctccgcccct gtgctggagg agggcgacac cgatccttgg acactgccac agctgaagga
     1141 cacatctcag ccatggaagg agctgagggt ggcaggccgg ctgaggagag tggcaggaag
     1201 cgtgctgaag gcatgcggac tgctgggctc cctgtacttc tttatctgtt ctctggatgt
     1261 gctgtctagc gcctttcagc tgctgggatc caaggtggca ggcgacatct tcaaggataa
     1321 cgtggtgctg tctaatcctg tggcagggct ggttataggg gttttggtga ccgccctggt
     1381 gcagtcctct agcacatcct ctagcatcgt ggtgtctatg gtggccgcca agctgctgac
     1441 cgtgagggtg agcgtgccca tcatcatggg cgtgaacgtg ggcacaagca tcacctccac
     1501 actggtgtct atggcccaga gcggcgacag agatgagttt cagagggcct tctccggatc
     1561 tgccgtgcac ggcatcttta attggctgac cgtgctggtg ctgctgccac tggagagcgc
     1621 cacagccctg ctggagagac tgtctgagct ggccctggga gcagcaagcc tgaccccaag
     1681 ggcacaggca ccagacatcc tgaaggtgct gaccaagccc ctgacacacc tgatcgtgca
     1741 gctggactcc gatatgatca tgtcctctgc caccggcaac gccacaaata gctccctgat
     1801 caagcactgg tgcggaacca caggacagcc aacccaggag aactctagct gcggcgcctt
     1861 tggcccctgt accgagaaga atagcacagc ccctgccgac agactgccat gcaggcacct
     1921 gttcgccggc accgagctga cagatctggc agtgggatgt atcctgctgg caggaagcct
     1981 gctggtgctg tgcggctgtc tggtgctgat cgtgaagctg ctgaactccg tgctgagagg
     2041 aagggtggca caggtggtgc ggaccgtgat caatgccgat tttcctttcc cactgggctg
     2101 gctgggagga tacctggccg tgctggcagg agccggcctg acctttgccc tgcagtcctc
     2161 tagcgtgttc acagcagcag tggtgcctct gatgggagtg ggcgtgatca gcctggacag
     2221 ggcctatcca ctgctgctgg gctccaacat cggaaccaca accacagccc tgctggccgc
     2281 cctggcaagc ccagcagata ggatgctgtc cgccctgcag gtggccctga tccacttctt
     2341 tttcaatctg gccggcatcc tgctgtggta tctggtgcct gccctgaggc tgccaatccc
     2401 tctggcaagg cacttcggag tggtgaccgc aaggtacaga tgggtggcag gcgtgtatct
     2461 gctgctgggc tttctgctgc tgccactggc agccttcggc ctgtccctgg caggaggcat
     2521 ggagctggcc gccgtgggcg gccctctggt gggcctggtg ctgctggtca tcctggtgac
     2581 cgtgctgcag aggcgccggc ccgcatggct gcctgtgaga ctgaggtcct gggcatggct
     2641 gcccgtgtgg ctgcactctc tggagccttg ggaccgcctg gtgacacggt gctgtccatg
     2701 caacgtgtgc tcccccccta aggccaccac aaaggaggcc tactgttatg agaatcccga
     2761 gatcctggcc tctcagcagc tgtaagaccc agctttcttg tacaaagttg gcattataag
     2821 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2881 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2941 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     3001 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     3061 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     3121 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     3181 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     3241 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     3301 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     3361 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     3421 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     3481 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     3541 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     3601 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3661 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3721 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3781 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3841 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3901 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3961 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4141 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4201 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4261 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4321 gtcgggctga acggggggtt cgtgcacaca
//
