LOCUS       sequence-479337-        4041 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A11_STOP
FEATURES             Location/Qualifiers
     source          1..4041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(151..250)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(267..286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(268..286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(291..307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(291..307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(409..428)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             420..1229
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(487..506)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1376..1964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1865..1884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2118..2135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2294..2321)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2413..2499)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2548..2570
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2562..2579
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2563..2579
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2595..2694
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcgccatcta taccggcctg ttcctgccag agaccaaggg caagacattt caggagatct
       61 ccaaggagct gcacaggctg aatttcccta ggagggcaca gggaccaacc tggcgcagcc
      121 tggaagtgat ccagtccaca gagctgtaag acccagcttt cttgtacaaa gttggcatta
      181 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      241 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
      301 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
      361 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      421 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
      481 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
      541 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      601 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
      661 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
      721 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      781 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
      841 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
      901 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
      961 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1021 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1081 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     1141 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1201 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     1261 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     1321 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1381 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1441 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1501 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1561 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1621 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1681 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1741 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1801 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1861 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1921 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1981 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2041 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2101 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2161 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2221 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     2281 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     2341 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     2401 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     2461 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     2521 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     2581 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     2641 acattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccacca
     2701 tgagggccct gcggagactg atccagggac gcatcctgct gctgaccatc tgcgcagcag
     2761 gaatcggagg cacattccag tttggctaca acctgagcat catcaatgcc cccaccctgc
     2821 acatccagga gttcaccaac gagacatggc aggcaaggac aggagagcca ctgcctgacc
     2881 acctggtgct gctgatgtgg agcctgatcg tgtccctgta tccactgggc ggcctgtttg
     2941 gcgccctgct ggcaggacca ctggcaatca ccctgggccg gaagaagtct ctgctggtga
     3001 acaatatctt cgtggtgagc gccgccatcc tgttcggctt ttctcggaag gccggcagct
     3061 ttgagatgat catgctgggc agactgctgg tgggagtgaa cgcaggcgtg tccatgaata
     3121 tccagcccat gtacctgggc gagtctgccc ctaaggagct gaggggagca gtggcaatga
     3181 gctccgccat cttcacagcc ctgggcatcg tgatgggcca ggtggtggga ctgagggagc
     3241 tgctgggagg accacaggcc tggcccctgc tgctggcctc ttgtctggtg cctggcgccc
     3301 tgcagctggc atctctgcca ctgctgccag agagccctcg gtatctgctg atcgactgcg
     3361 gcgataccga ggcatgtctg gccgccctga ggcgcctgag aggctccggc gacctggccg
     3421 gcgagctgga ggagctggag gaggagcggg ccgcctgcca gggctgtaga gcccggagac
     3481 catgggagct gttccagcac cgggccctga ggcgccaggt gacatccctg gtggtgctgg
     3541 gctctgccat ggagctgtgc ggcaacgata gcgtgtacgc ctatgcctct agcgtgttta
     3601 gaaaggccgg cgtgcccgag gccaagatcc agtacgccat catcggaacc ggaagctgcg
     3661 agctgctgac agcagtggtg tcctgcgtgg tcatcgagag ggtgggccgg agagtgctgc
     3721 tgatcggagg ctactccctg atgacctgct ggggctctat cttcacagtg gccctgtgcc
     3781 tgcagtcctc ttttccttgg accctgtatc tggccatggc ctgtatcttc gcctttatcc
     3841 tgtccttcgg catcggacca gcaggagtga ccggaatcct ggccacagag ctgtttgatc
     3901 agatggccag gcctgcagca tgcatggtgt gcggcgccct gatgtggatc atgctgatcc
     3961 tggtgggcct gggcttccct tttatcatgg aggccctgag ccacttcctg tacgtgccat
     4021 ttctgggcgt gtgcgtgtgc g
//
