LOCUS       sequence-479346-        3921 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC29A2_STOP
FEATURES             Location/Qualifiers
     source          1..3921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(931..1740)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1654..1673
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1732..1751
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1853..1869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1853..1869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1874..1893
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1874..1892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1910..2009
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3387..3486)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3502..3519)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3502..3518)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3511..3533)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3582..3668
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3760..3787
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
      841 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg
      901 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
      961 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     1021 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     1081 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     1141 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     1201 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     1261 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     1321 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     1381 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     1441 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     1501 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     1561 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     1621 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     1681 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     1741 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     1801 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gccaggaaac
     1861 agctatgacc atgtaatacg actcactata ggggatatca gctggatggc aaataatgat
     1921 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat
     1981 aatgccaact ttgtacaaga aagctgggtc ttacagcagg gccttaaaca ggaagctcag
     2041 agatgctcca caggacagtc ccagggccag aaagaaggtc atcagggcgc ctgccacctc
     2101 tctctcgtgt ggcagcacct gcctaggtgc caggcacatt gtcagggaca ccagatagcc
     2161 gttgctcacg gcaaacagca gcataaaggt gatgaagtag gcatcctggg ggaacaggat
     2221 tggcagtctg gaccgctgtg gcacgtggca cagcataaac agtggcacaa acaggaagcg
     2281 caggcacacc agcagaggca gcagcctgct atcctcgtcg ggccacagga agtagctggt
     2341 cagagaccgg cccagccagt ccatgatgtt aaacagcagg aaacagcaga taggattaaa
     2401 gaactgggac cacttgccgg ggcttgtaga ggaggtcacc atggctgtga tggcaggaaa
     2461 cacgctcagt gtcacggtga acaccagcac caggcacagg gctgtcagcc agatcttctg
     2521 aaacacggtg aacacggaag gcttgccggg cttctgtggc tcatcaggct cgctctcggg
     2581 ctccttctcc agatccaggt ccagggtcag ggccaccttc tgtgggctag atgggatgcc
     2641 gttctcgtcg gactgcagca gctcggcctt tgtctccagc tcctgtgcct gtgcctggga
     2701 gctcttattg gccagatagt agcgggcaaa cttcaggtgg ggcagagaca gatagcacac
     2761 gatggacatc aggatgccca cgcatggtgt gatgaagtag cccagggcgc tggtctctgc
     2821 gtccactccg gatgccatgc tcagcagcat tgccagggcg gcaaagattc ctgccagtcc
     2881 ctgtccgctc aggaacagtg tagaataggt ggagggcatg gtgcccagct gtccgaacag
     2941 agatccctgc agcacggcgg aaaagctgtt gatgaagcac acgctggcca tggtgatgga
     3001 aaagaaaggg ccgggagaca tatccacctt caccagggcg gctgtcaggg cgaacagcag
     3061 caggatggcc agcagggagc ccaggatcct cacggtctct ggcacgcact ggtacagaaa
     3121 agaattcagc agtgtgaaca gcagcagagg cagctgggac agcagggtca cccaattgtt
     3181 aaagttgaag gcgtcctcag ggcctgtgtg attggtgctc aggattctgg ctgtagagtt
     3241 tcctgctcct gccagccttg cctggaaata tgggatggct gtgataaaga aattccaagg
     3301 cagcagggtg cccaggccca ggataaagaa gctgatgccc accaggtggt aagaatcgcg
     3361 tggggcgtcg cccctggcca tggtggagcc tgcttttttg tacaaagttg gcattataaa
     3421 aaagcattgc tcatcaatgt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     3481 tatttggggc ccgagcttaa gactggccgt cgttttacaa cgtcgtgact gggaaaacat
     3541 ccatgctagc gttaacgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     3601 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     3661 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcggg
     3721 caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc ctgacggatg
     3781 gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa ttgcgttgcg
     3841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3901 acgcgcgggg agaggcggtt t
//
