LOCUS       sequence-479408-       13025 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Used for microinjection of nematodes for expression of PDZD8-T527E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 13025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pHM1 PDZD8-T527E
FEATURES             Location/Qualifiers
     source          1..13025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     183..200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    317..338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(353..358,359..376,377..383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     396..418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     415..431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     415..431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9298..9326)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10074..10094
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(10171..10195)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11021..11043)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11143..11162
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11244..11262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11330..11434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11435..11503,11504..12295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11653..11672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12466..13025,1..29)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12955..12974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
       61 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      421 acagctatga ccatgattac gccaagcttg catgcgcggc cgcactgact gggccggcct
      481 tctttgacag cgaaattgag taataggcga actttattat aagtcttaca cccccatagc
      541 tccatttgca tgtctcatcg ctgtctttaa gttaaacttt ttttttgaaa aaatcatttc
      601 aatcattcaa aatacgtgtc aaggcgtctc ttcttttaaa tattttaaaa gtcaactaat
      661 ttgtgataaa ctcaaagaga atcgagatat gagctgtcaa agtagatcaa cttgaaaaaa
      721 gaagttggtg cataccagtt gtactttttg tgaagtttac tactagttaa tataaaaaaa
      781 taaaaattga catagattta ttaaaattat cacagttcga atcgatcaca gttcaagatc
      841 attaaaatat tctattcggt actacactct aaactcaatt aatccgtttt gtgtttctta
      901 atgagattct cgtcgaccgg cgacacaacc ataaatgata ttgctaaaag agatccaaca
      961 attccaagaa gagtcagtga ttggaatcca tagttttgga gaagataacc agcaaaaagt
     1021 ggagagaaag tacgaacaag agagtttaaa gatgtggccg tgcccagtac gattccctga
     1081 aatgttgaat tacctcattt gaaacactgt gaataatgag tggaactgaa ctaaaagaaa
     1141 actcctaacc tgttcagatt catcaactaa agctgtgaga agactatctg cacatgttgc
     1201 aactacactc attccaagag aaacaaatgg cattataaca agaagcatcc agaaattgaa
     1261 aaagacaaat gtgaataaag tgtagccaat aacctggaaa taatcataca taaactttga
     1321 atctttagca caactttaaa gtaaatgtaa cttacaaaac tcaacgatcc gattattaaa
     1381 agtgtttgct catcaaattt ttttctaagc caaatgactc cgaatccatt agagaacata
     1441 ataaatacac ccatcatcat ctgcattatt tgattttctg tattggtagc attgaatttc
     1501 tcaatgatgt ataactgtaa aaacgctaga gtttaaattg tgaaattaac aatttgaaac
     1561 tcacattcag tactgcaatt atcataatga aaggaacaac tccaccattt ttcttaatca
     1621 taacatttgc tactccaggc ttttgaagaa tgcgaacaac atttgtaatg tttatttgag
     1681 gtggtggacc atttgcattg tccgttgaga tttcatgctg gaaaattttt aaagcttata
     1741 atttctagat atgaaaactg gtggatatat catatatttt gaaggtggag tattggtcgt
     1801 ggaagcctga ttacatataa aatcgtgtag atttttcaaa aactttcagt cattgtgttg
     1861 gtaaatttcc aaattaaaaa aaaaaacttt ttagaaatat caattactgc tgcaggtctg
     1921 aaccccatag tacatgaatg cagattatta gaatatagca atcgaagaaa aaatttttgg
     1981 tcgacttcta gaattcgata cttcactttt aacaatagcg tgtgttttgc aactaacctc
     2041 atatgatttt ttctcaatac acgtttccaa aactaatgat ggaagagcac aaagtgcagc
     2101 agaaaccaga agtggagcaa gatctcctcc aagttttgtg gcagcaattg agaatattgg
     2161 tacaaagatg aaacctattc caaatgtaag tcccattctc ccaaatgcac ttgttctctc
     2221 ttttccgggt gttgtaagtg ctgataaaag agtttgatgg gcttgctgtc catgcataaa
     2281 aactacggga atacgagata gtagaagaga ataataatcc tgaaatttga ctattattta
     2341 tcttaagcct aagatatctt gaaagaaaat tcagaaaatc gaggatagac tttacattag
     2401 gactggaatt cctgggattt tgccttaaat ggctccaaat tatgtgtcat tttaaaattt
     2461 atcaattcaa gaaaatttac attagtttcg aaaaagccaa caattgggcg ggacatgctc
     2521 atgtctgtag gggacaaata gttggctcta atatttctag atttgtttga tttttctttg
     2581 ttgaatatga tatactaggg tattttggag caattcataa aaaaaccgca caagtatttt
     2641 tcaattttgg aaaaggttca agttttagat ttttcactaa aacaactttt ttgatatttt
     2701 tgaaaactga agataatatt aaatacgtca taatgaagca cactcaaatt taactgcaga
     2761 aattttggag tttttatttt caacttaacg cgaagtcgtg tctaatagta agaatccaaa
     2821 aagtttgagt aacttacaaa tgaaaaataa agtgctaaac cagaacaaag agtagacaga
     2881 tagcataaat gaagtgcata tcgaatacca aacttttgaa tcacatatcc aaaaagggga
     2941 ccaccaatca tttgaaaaac tccgaatatt gtttgaatgt accctgaatt tcactttaaa
     3001 tttcttacaa ttcaaaaaaa gtttttcttt taagtgtttt catattcgac ttaccaaaat
     3061 ccgcatcgga aagttgtaga tgtttaacta aatatggcgt agcagaaaat tgcaggaaaa
     3121 atgtaacatt atagaaaata gtaagtaagt atgtaaaagc aataggccga gaaacttcga
     3181 aagatcctat ttttatagta tttggcaaca acgccgtcat cttctgagtt ctgaaattcg
     3241 gaaaaaattg cacttcgtct gaaactaagt tttaactata gagaggaact gattgatagc
     3301 gagactactc tccaatgggg aaaagaagtg aactatgtgt ttgattatca gtccttttaa
     3361 tgttgttttt gtggtttgta cccttttcct atcacaaata tgatcagttg caataagaaa
     3421 aaaacacaat tttttctggt ttttcaatag gtttatagcg gttgatataa tgttatagaa
     3481 ttgtgaaatt atagcgatga aaacttcggt atgcttacat tcgatttttg gaattttcag
     3541 tgaagaaata ccaatgtcta attcaaaatt cagatttaaa ttgggtttgc attcgcttta
     3601 aaaatccaac gatggaaata tggaagagaa atcaattggg gaatcctagt ccgcgcggcc
     3661 gtgtagtcct cttggaccag cggcacttcg atacattttt ttctgttttc tccattttcg
     3721 gcgcacactc tcgtgaaaat aagcgtgacc tgctttgttt acactttttt gaatcatttt
     3781 ttattagaat atcgtaaatt tgtaacaaaa tgctagataa atacccaaaa actagtaatt
     3841 ttcaagcatt ttcctcttat gatttttctc tcgtgtatta catttctgtt cactcgctgc
     3901 atatttccga taatttctat aaaagtacat cattattacg aaaagaagac atcattcttt
     3961 gcttcaggaa gcgaacaagc tgctcaatca cgctgaaaag cacaaaacgc tcttttctcc
     4021 atttttctct ccgcgttttt ttccgttttc gtttcaattc cattttcctc gcaggcattc
     4081 cccaaaaaca tgtcacacat ttgagatccc aattcatttt ggcagaaacc cggataatcc
     4141 ccctttgttt gggtgctttc tcatttctgg gcactttctt ttctaatttt ggacgaagaa
     4201 acaaaacaag aaaagattta catttccttt ctcccgtttg tctccgtttt tttctttttc
     4261 tttatgctat caaaagctct cattagtaaa aagagaaaga ttttatcgag gaaacagaaa
     4321 tgtgagagtt ttgaaatcga gaagtacacc aattttcgca tagattctgc tttcttcctt
     4381 aaaattcgtt gaagctaata agaaaaatcc taaagtctat tctgagaaga tatataattt
     4441 taaagtgttt attatttaga tagatcggaa atattaagtg ttcacaacaa aaaatcaccg
     4501 aggttcccac agctgactct tcttttttgc atattttttc attttctctt ttttccattt
     4561 cccctccaaa caataatcat cacatttccg tgctttccac cactttctct tttgaagcgt
     4621 tcttctccaa gttgtccggt tgatagattg ctgatagact gatgacaaat cgacacaacc
     4681 taccaaccgg ctgatttttt ttgaaaagaa gaagcagaca atttttttct gcttttggaa
     4741 ggtctggttg ttcaacagca gtttaagttt ggaatactgc tgattgtttc aaagtttgtt
     4801 tttttaacac ctgcaatttc tagcaatata aacgaagaaa cgtattcctt ctgatagatt
     4861 tttgtacttt atagcattat gcagatattt atgagccaat attatattga acattaaaaa
     4921 ttttcccgac aaaatcagtt tcgatgacgt agtctgacgt gtgtaacaga agaaccagaa
     4981 aagacacgtg gagctcttcg aaggtcatat gaaactattg taggtgctgg gatatagggt
     5041 cacctgattg taagctcttc tcgaaatcag acacgacacg tttctttttt ttcaatatct
     5101 atgatatctc taatctctct tctcttttat ctgttttcta tctctctact agaggatcac
     5161 tctcgtagta acaacaagaa aacataattc cccatcttag aaacgtgtgc tccgttgtca
     5221 agtcgcatat gtcacagacg aaacaagcaa aaacaataaa aacttggaaa atcgtatttt
     5281 gttcgcttgc tgtttcatat tttactattt tgagaaaaag ctatttctcg tgatccgcct
     5341 tcttcttttg ttcatgaaaa tacgaaaatt attttaaata atttctttat ttttctagct
     5401 caactgtctt agttacgatt cttcttaaac tatattattt ttcagggcgc gcctctagag
     5461 gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
     5521 gaacattttc aggaggaccc ttggctagca tggggctgct gctcatgatc ctggcgtcgg
     5581 ccgtgctggg ttccttcctc acgctcctcg cccagttctt cctgctgtac cgcagacagc
     5641 ccgagccgcc ggcggacgag gccgcccgcg cgggcgaggg cttccgctac atcaagccag
     5701 tgccgggcct gctcctaagg gagtaccttt atggcggcgg ccgggatgag gagccctccg
     5761 gagcggcccc tgagggcggc gcgaccccca ccgcggcccc cgagaccccc gccccgccga
     5821 cgcgggagac ttgctacttc ctcaacgcca ccatcctatt cctgttccgg gagttgcggg
     5881 acaccgcgct gacccgccgc tgggtcacca agaagatcaa ggtggagttc gaggagctgc
     5941 tgcagaccaa gacggccggg cgcctgctgg aggggctgag cctgcgggac gtgttcctgg
     6001 gcgagacggt gcccttcatc aagaccatcc ggctcgtgcg gccagtcgtg ccctcggcca
     6061 ccggggagcc cgatggccct gaaggggagg cgctgcccgc cgcctgcccc gaggagctgg
     6121 ccttcgaggc ggaggtggag tacaacgggg gcttccacct ggccatcgac gtggacctgg
     6181 tcttcggcaa gtccgcctac ttgtttgtca agctgtcccg cgtggtggga aggctgcgct
     6241 tggtctttac gcgcgtgccc ttcacccact ggttcttctc cttcgtggaa gacccgctga
     6301 tcgacttcga ggtgcgctcc cagtttgaag ggcggcccat gccccagctc acctccatca
     6361 tcgtcaacca gctcaagaag atcatcaagc gcaagcacac cctaccgaat tacaagatca
     6421 ggtttaagcc gttttttcca taccagacct tgcaaggatt tgaagaagat gaagagcata
     6481 tccatataca acaatgggca cttactgaag gccgtcttaa agttacgttg ttagaatgta
     6541 gcaggttact catttttgga tcctatgaca gagaggcaaa tgttcattgt acacttgagt
     6601 taagcagtag tgtttgggaa gaaaaacaga ggagttctat taagacggtt gaattaataa
     6661 aaggaaattt acaaagtgtt ggacttacac ttcgtcttgt ccagtcaact gatgggtatg
     6721 ctgggcacgt catcattgaa actgtggctc caaactcgcc tgctgcaatt gcagatcttc
     6781 agcggggaga tcgacttatc gccattggag gtgtgaaaat cacatcaaca ctgcaagtgt
     6841 tgaagcttat caagcaggct ggtgaccgag tcctggtgta ctatgaaagg cctgttggcc
     6901 agagtaatca aggtgcagtg ctgcaagata actttggcca gttggaagaa aactttttgt
     6961 caagctcatg ccaatcgggt tatgaagagg aagctgccgg gttgacagta gatactgaaa
     7021 gtagagagct ggattctgaa tttgaagact tggcaagtga tgtcagagca caaaatgagt
     7081 tcaaagatga ggcacaatca ttaagtcata gtcccaaacg tgttccagag acactttcta
     7141 ttaaacccct tggagctata tcaccagttt taaaccgtaa attagctgta ggaagtcacc
     7201 cactaccacc gaaaattcag tccaaagatg gaaataaacc tccaccccta aaaacttctg
     7261 agataacaga cccagcacaa gtgtcaaaac caacccaagg atctgctttc aaaccacctg
     7321 tgccaccacg accacaagcg aaagttcctt tgccttccgc cgatgctcca aatcaggcag
     7381 aaccagatgt tctcgttgaa aagccagaga aggtggtgcc acctcctctt gtagataaat
     7441 ctgctgaaaa gcaagcaaaa aatgtggatg ccatagacga tgcagctgca cctaagcaat
     7501 ttttagcaaa gcaagaagtg gccaaagatg tcacttcaga aacttcctgc cctactaagg
     7561 acagttcgga cgaccgtcaa acatgggaat catcagaaat tctttatcgt aataagctag
     7621 gaaaatggac aagaaccaga gcatcctgtt tgtttgacat agaagcctgt cacaggtact
     7681 taaacattgc attgtggtgc agggatcctt tcaagttggg aggtctcatc tgtttggggc
     7741 atgttagttt aaaacttgaa gatgtggctt taggatgcct agctacatca aacacggaat
     7801 acctttccaa attgagactg gaagccccct cacctaaggc tatagtcact agaaccgcac
     7861 tacgcaatct gagtatgcaa aagggattca atgacaaatt ttgctatggt gacattacta
     7921 ttcacttcaa atatttgaaa gaaggagaat cagaccacca tgtagttact aacgtagaaa
     7981 aagaaaaaga accccatttg gttgaagaag tttctgttct ccctaaagag gagcaatttg
     8041 ttggacagat gggtttaaca gaaaacaaac acagttttca ggatactcag ttccagaacc
     8101 caacatggtg tgactactgt aagaaaaaag tttggactaa agcagcttcc cagtgtatgt
     8161 tttgtgctta tgtttgccat aaaaaatgtc aagaaaagtg tctagctgag acttctgttt
     8221 gtggagcaac tgataggcga atagacagga cactgaaaaa ccttaggctg gaaggacagg
     8281 aaaccctctt aggcctgcct cctcgtgttg atgctgaagc tagcaagtca gtcaataaaa
     8341 caacaggttt gacaaggcat attatcaata ctagttctcg tttattaaat ttgcgtcaag
     8401 tctctaaaac tcgcctttct gaaccaggaa ccgatctcgt agaaccttca ccaaaacaca
     8461 cacccaacac gtcagacaac gaaggcagtg acacggaggt ctgtggtcca aacagtcctt
     8521 ctaaacgggg aaacagcaca ggaataaagt tagtgagaaa agagggtggt ctggatgaca
     8581 gtgttttcat tgcagttaaa gaaattggtc gtgatctgta caggggcttg cctacagagg
     8641 aaaggatcca gaaactagag ttcatgttgg ataagctaca gaatgaaatt gatcaggagt
     8701 tggaacacaa taattccctt gttagagaag aaaaagagac aactgataca aggaaaaaat
     8761 cacttctttc tgctgcctta gctaaatcag gtgaaaggct acaagctcta acacttctta
     8821 tgattcacta cagagcaggc attgaagata tagaaacttt agaaagtctg tctttagacc
     8881 agcactccaa aaaaataagc aagtacacag atgatacaga agaagacctt gataatgaaa
     8941 taagccaact aatagactct cagccattca gcagcatatc agatgactta tttggcccat
     9001 ccgagtctgt gggtaccgct gtctcatcct actttcacct agttaactgc ttgtcttaaa
     9061 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
     9121 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
     9181 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
     9241 agggaaactg ctgtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt
     9301 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     9361 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     9421 aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta
     9481 aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac
     9541 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     9601 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc
     9661 ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat
     9721 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     9781 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     9841 aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta
     9901 aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     9961 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
    10021 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
    10081 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaatagcat
    10141 tcgtagaatt ccaactgagc gccggtcgct acccagcttt cttgtacaaa gtggtgatat
    10201 ctgagctccg catcggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc
    10261 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
    10321 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
    10381 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
    10441 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
    10501 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
    10561 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
    10621 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
    10681 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt
    10741 ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt gctaaaatca
    10801 caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt
    10861 ggaaggtgag tagaagttga taatttgaaa ttggagtagt gtctatgggg tttttgcctt
    10921 aaatgacaga atacattccc aatataccaa acataactgt ttctactagt cggccgtacg
    10981 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    11041 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    11101 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    11161 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    11221 gcatcaggcg gccttaatta agggcctcgt gatacgccta tttttatagg ttaatgtcat
    11281 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    11341 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    11401 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    11461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    11521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    11581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    11641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    11701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    11761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    11821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    11881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    11941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    12001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    12061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    12121 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    12181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    12241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    12301 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    12361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    12421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    12481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    12541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    12601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    13021 gtgat
//
