LOCUS       sequence-479409-       11356 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Used for microinjection of nematodes for expression of PDZD8-T527A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pHM1 PDZD8-T527A
FEATURES             Location/Qualifiers
     source          1..11356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     183..200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    317..338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(353..358,359..376,377..383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     396..418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     415..431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     415..431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7629..7657)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8405..8425
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(8502..8526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9352..9374)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9474..9493
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9575..9593)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9661..9765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9766..9834,9835..10626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9984..10003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10797..11356,1..29)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11286..11305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
       61 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      421 acagctatga ccatgattac gccaagcttg catgcgcggc cgcactgact gggccggcct
      481 gctaaattgc aaccccgtgt ttttgaaaaa atacatcata acagagaaaa ctctttaaaa
      541 tcatattgtt cagccaggtc acagtcaagg acaattttga atttgactca atttcagtta
      601 caggctcgga taacggcgct gccacgacca ccctggcccc ttctgaccca acttgttaaa
      661 tattaatcta aatacaaata agatttagta tgaacattta ctgttcattt tgaatgttgt
      721 ttttttacac attttcgtag ttgaaaattt tgacatattt taagtgttgg aaaagatatg
      781 agacatttga gcgaactcgt ccaaatttgt gaaaatttcc aaaaatcatc gtgaaaaatt
      841 aaaaaacata ttttcagaga aaaggcttaa acatttttga atatgaataa gtaacctttt
      901 ttaaaaatcg gcatgttttt ttctttgaag atcaatattt ttgcgaaagc ctacgatact
      961 aaaaaatatt aacagcaaaa aggtgtaaaa ttaaaaagtg attgtttcat ctgttgacaa
     1021 aagttttgtc aaacaactgg ttttggaatt tatatgcaaa aacctgtaaa aagagtgttt
     1081 attgaatctt ggacttgact tgtttcatcg gactgtaatt tagcaggggc ttaagatagg
     1141 atgaattggt aaagtgataa aatatagcta ttggtatgca aaatattgtt acaattgatt
     1201 actttttggt atcattcacg tttctgaagt taccgccact caaagttaaa taaaataatt
     1261 tttgcaagca tttttgacaa cttaaacgaa tatggcggga ccaaaaaggc catgcctgga
     1321 gctgttattg catgtttttg ataaacttac tgtcatacat cagggtacat aaggtttttg
     1381 atttcacaca aagaaaaaaa aatcgaaaaa aaaacaattt ttttccgttg tcatagttga
     1441 aaaaccctgt tgctaaacaa ttcttctaat ttttcgtggt aactgctaca caatcggatt
     1501 aaggaaacct aattttttct cacatatgtt ggagagcctc gatattcttg aactttttgc
     1561 attcattgtt taatgtttct tcacatagct tcgtatatat tgccagcaat gcaaatcttg
     1621 agccatcggt tgaaatgatt ttgaaaaggt gctcggcata tctgggctga aaaaaaactt
     1681 ttgtttcaac gcattaaaca cttttcgggg ttctgttcca attttttaaa tagttgtaac
     1741 acccgctaga atttccgttt caccaaagaa atctttgttc acagattatt atatatttct
     1801 tttttatgta ttttgtccag gtgatcctaa atgtttaaaa aacgttccag cagcttttag
     1861 tctatacgaa gtatattttt tcttattttg gtttatcgga ggtatgcagt tccaaatgtt
     1921 ggattttcta ccctactaga aatttccttt agttatacaa ttttaattcg aagttgtatc
     1981 ttgaaaagga aaccatcatc aacaacaaat aagcttgaga ctagaattgc taatcgtctg
     2041 caccaaatat gaaattcgtt gaataaatta tataaaacag ttgacaatca taaagtattt
     2101 tacatacata tgtatagtta atgtcatatt agcatattta gtattgagtt aacacgcaga
     2161 aataccaaag aaaccctgtt taattcattt ccatgtttta ttcgctttaa ctagggaatg
     2221 accagaataa gtggagcgat attcaaaaaa aaaaattgta tcggaaagct gacattctct
     2281 actataagaa tatgactgaa atttttgccc attcgggctg gaaatctgaa tggaaattgt
     2341 ttatatgagg ggatccatgg ttgtagttac aattttgaga aaaaagttta aattttaggg
     2401 gaaaagatgt tgaatgaatc attatggtgt tggtcgacac attgatcatg ctgatatcga
     2461 tcagaaaaat taaatttggg cttttatata gtttcgaaaa aacacgttta aatttttgaa
     2521 aaattttacg ttagggtgga attgaacccc agagtcccac catgcttgtc atccactcta
     2581 tccactcggc catctcgctg ttgcaggcag tactaattgg ggatagtgaa aagagttaac
     2641 gtagaatttc agacgtaaaa atttcagatt tccagcccga atgggcaaaa atttcagtca
     2701 tattcttata gtagagaatg tcagctttcc gatacaatat tttttttttg aatatcgctc
     2761 cacttattct ggtcattccc tagtaagttt tttcagtttt accagaaata aaaccactca
     2821 taaaaactat aaactacaca tttaaactgt aaatgttgga aatcattttg accatcaaac
     2881 aatagctatc ggaaaatgca aaggccattc cgtgatggca cgctcttctt ctatccatcg
     2941 ctccttttca acgaaacaca cacacaaacg cagactattg gaaaagaaaa atatagaaaa
     3001 aacctttttt ctccttgtcc cttgtgttga tctcactttt cagtggacgt gtgcagtgtg
     3061 gaggccagag actgtgctga ccgacggacg cgcaggcggt aaacatacgt tgcagtggag
     3121 agtgtagtcc ctcgtcggct ctctgtgtat ttctttcaat gcctgtgtct cggtctatct
     3181 ttgtctctat ctgttttaaa agttttttgc aaagtttcaa tttctttttt attttctctt
     3241 ctttttttgc gcatttattt tttttaaaga gcgctgttct tcaattggaa aacagtataa
     3301 cgatcgaaat aaaaaaaaaa cttgaaatag tatcggaatt gagatggggg caattgaaat
     3361 tgaagacgca acaaaaacat gtttttatcg tctgaaatta tctaaacttt gaatctaacg
     3421 tgtcaatggc tacttttaca gtagactttt tagagtaatg ttaaaaagga taatataggt
     3481 ttttcttcaa actattttgt cacgccgcat atattctttt catattccat tcgcaaagtg
     3541 catgatgtaa caaaaatgaa acgtcataat tttttaatct ttcacacttt ttttgccggc
     3601 cgttaaatca aaatcaaatc aacttaatcc aacacgtgcg ggcttgcaat tttattacac
     3661 cggtgtccta cagcgccatg ttatcttctt tgattagaga tctctcacgc aacatttgac
     3721 attgaaaatt ttgtctccga attgttgtgt acctactttg tcccgatgtc ccaaattttc
     3781 actgataaca agagatatct caactgttct tatcacgttg atacgtagaa cgttttacac
     3841 taaaccaaat tgcagtcccc ttctacgaac cgtttgctaa ttgttttgca tagaacagtg
     3901 atcaatgtgt tgtgctcact agtcaatgat gttcgtctct catttttcct acaacttcac
     3961 tgaaaaggtt ttgaatatct ggtgattgta ggcaatcaca ccacagttaa tggcctggca
     4021 aataatgata agcgtgtgat aaacctcaca aaaatacgcc tagaagtggt gatatcgcta
     4081 gttttgtgtc ctttcgattt ttttcgtgtt gcttttttct gtggataaac ctttgaactt
     4141 tacattttgt tttcagaggc gcgcctctag aggatccccg ggattggcca aaggacccaa
     4201 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
     4261 catggggctg ctgcagacca agacggccgg gcgcctgctg gaggggctga gcctgcggga
     4321 cgtgttcctg ggcgagacgg tgcccttcat caagaccatc cggctcgtgc ggccagtcgt
     4381 gccctcggcc accggggagc ccgatggccc tgaaggggag gcgctgcccg ccgcctgccc
     4441 cgaggagctg gccttcgagg cggaggtgga gtacaacggg ggcttccacc tggccatcga
     4501 cgtggacctg gtcttcggca agtccgccta cttgtttgtc aagctgtccc gcgtggtggg
     4561 aaggctgcgc ttggtcttta cgcgcgtgcc cttcacccac tggttcttct ccttcgtgga
     4621 agacccgctg atcgacttcg aggtgcgctc ccagtttgaa gggcggccca tgccccagct
     4681 cacctccatc atcgtcaacc agctcaagaa gatcatcaag cgcaagcaca ccctaccgaa
     4741 ttacaagatc aggtttaagc cgttttttcc ataccagacc ttgcaaggat ttgaagaaga
     4801 tgaagagcat atccatatac aacaatgggc acttactgaa ggccgtctta aagttacgtt
     4861 gttagaatgt agcaggttac tcatttttgg atcctatgac agagaggcaa atgttcattg
     4921 tacacttgag ttaagcagta gtgtttggga agaaaaacag aggagttcta ttaagacggt
     4981 tgaattaata aaaggaaatt tacaaagtgt tggacttaca cttcgtcttg tccagtcaac
     5041 tgatgggtat gctgggcacg tcatcattga aactgtggct ccaaactcgc ctgctgcaat
     5101 tgcagatctt cagcggggag atcgacttat cgccattgga ggtgtgaaaa tcacatcaac
     5161 actgcaagtg ttgaagctta tcaagcaggc tggtgaccga gtcctggtgt actatgaaag
     5221 gcctgttggc cagagtaatc aaggtgcagt gctgcaagat aactttggcc agttggaaga
     5281 aaactttttg tcaagctcat gccaatcggg ttatgaagag gaagctgccg ggttgacagt
     5341 agatactgaa agtagagagc tggattctga atttgaagac ttggcaagtg atgtcagagc
     5401 acaaaatgag ttcaaagatg aggcacaatc attaagtcat agtcccaaac gtgttccagc
     5461 aacactttct attaaacccc ttggagctat atcaccagtt ttaaaccgta aattagctgt
     5521 aggaagtcac ccactaccac cgaaaattca gtccaaagat ggaaataaac ctccacccct
     5581 aaaaacttct gagataacag acccagcaca agtgtcaaaa ccaacccaag gatctgcttt
     5641 caaaccacct gtgccaccac gaccacaagc gaaagttcct ttgccttccg ccgatgctcc
     5701 aaatcaggca gaaccagatg ttctcgttga aaagccagag aaggtggtgc cacctcctct
     5761 tgtagataaa tctgctgaaa agcaagcaaa aaatgtggat gccatagacg atgcagctgc
     5821 acctaagcaa tttttagcaa agcaagaagt ggccaaagat gtcacttcag aaacttcctg
     5881 ccctactaag gacagttcgg acgaccgtca aacatgggaa tcatcagaaa ttctttatcg
     5941 taataagcta ggaaaatgga caagaaccag agcatcctgt ttgtttgaca tagaagcctg
     6001 tcacaggtac ttaaacattg cattgtggtg cagggatcct ttcaagttgg gaggtctcat
     6061 ctgtttgggg catgttagtt taaaacttga agatgtggct ttaggatgcc tagctacatc
     6121 aaacacggaa tacctttcca aattgagact ggaagccccc tcacctaagg ctatagtcac
     6181 tagaaccgca ctacgcaatc tgagtatgca aaagggattc aatgacaaat tttgctatgg
     6241 tgacattact attcacttca aatatttgaa agaaggagaa tcagaccacc atgtagttac
     6301 taacgtagaa aaagaaaaag aaccccattt ggttgaagaa gtttctgttc tccctaaaga
     6361 ggagcaattt gttggacaga tgggtttaac agaaaacaaa cacagttttc aggatactca
     6421 gttccagaac ccaacatggt gtgactactg taagaaaaaa gtttggacta aagcagcttc
     6481 ccagtgtatg ttttgtgctt atgtttgcca taaaaaatgt caagaaaagt gtctagctga
     6541 gacttctgtt tgtggagcaa ctgataggcg aatagacagg acactgaaaa accttaggct
     6601 ggaaggacag gaaaccctct taggcctgcc tcctcgtgtt gatgctgaag ctagcaagtc
     6661 agtcaataaa acaacaggtt tgacaaggca tattatcaat actagttctc gtttattaaa
     6721 tttgcgtcaa gtctctaaaa ctcgcctttc tgaaccagga accgatctcg tagaaccttc
     6781 accaaaacac acacccaaca cgtcagacaa cgaaggcagt gacacggagg tctgtggtcc
     6841 aaacagtcct tctaaacggg gaaacagcac aggaataaag ttagtgagaa aagagggtgg
     6901 tctggatgac agtgttttca ttgcagttaa agaaattggt cgtgatctgt acaggggctt
     6961 gcctacagag gaaaggatcc agaaactaga gttcatgttg gataagctac agaatgaaat
     7021 tgatcaggag ttggaacaca ataattccct tgttagagaa gaaaaagaga caactgatac
     7081 aaggaaaaaa tcacttcttt ctgctgcctt agctaaatca ggtgaaaggc tacaagctct
     7141 aacacttctt atgattcact acagagcagg cattgaagat atagaaactt tagaaagtct
     7201 gtctttagac cagcactcca aaaaaataag caagtacaca gatgatacag aagaagacct
     7261 tgataatgaa ataagccaac taatagactc tcagccattc agcagcatat cagatgactt
     7321 atttggccca tccgagtctg tgggtaccgc tgtctcatcc tactttcacc tagttaactg
     7381 cttgtcttaa aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta
     7441 tataaatggt ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt
     7501 tcctcgtttt tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct
     7561 aattttaatt cagggaaact gctgtaccgg tagaaaaaat gagtaaagga gaagaacttt
     7621 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     7681 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     7741 gcactactgg aaaactacct gttccatggg taagtttaaa catatatata ctaactaacc
     7801 ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt gttcaatgct
     7861 tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
     7921 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtaagt
     7981 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct gaagtcaagt
     8041 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     8101 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
     8161 cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta ctaactaact
     8221 aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     8281 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     8341 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     8401 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     8461 acaaatagca ttcgtagaat tccaactgag cgccggtcgc tacccagctt tcttgtacaa
     8521 agtggtgata tctgagctcc gcatcggccg ctgtcatcag atcgccatct cgcgcccgtg
     8581 cctctgactt ctaagtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     8641 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
     8701 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     8761 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     8821 acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt tttgaaacat
     8881 catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt tatgaccgca
     8941 atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc atcgtgaaaa
     9001 agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat taattttcct
     9061 gcttttgctt tttggggttt cccctattgt ttgtcaagat ttcgaggacg gcgtttttct
     9121 tgctaaaatc acaagtattg atgagcacga tgcaagaaag atcggaagaa ggtttgggtt
     9181 tgaggctcag tggaaggtga gtagaagttg ataatttgaa attggagtag tgtctatggg
     9241 gtttttgcct taaatgacag aatacattcc caatatacca aacataactg tttctactag
     9301 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9361 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9421 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9481 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9541 gagaaaatac cgcatcaggc ggccttaatt aagggcctcg tgatacgcct atttttatag
     9601 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     9661 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9721 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9781 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9841 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9901 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9961 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    10021 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    10081 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10141 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10201 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10261 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10321 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10381 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10441 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10501 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10561 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10621 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10681 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10741 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10801 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10861 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10921 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10981 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11041 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11161 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11341 cgtcgatttt tgtgat
//
