LOCUS       sequence-479447-        6550 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC PDZD8 
            (91-449aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC PDZD8(91-449aa)
FEATURES             Location/Qualifiers
     source          1..6550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     495..514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(733..837)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(912..931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1103..1482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1483..1686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1637..1657
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1683..1707
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2914..2943
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2989..3008)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3033..3081
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3283..3711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3370..3389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3580..3601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3720..3740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3725..4054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3905..4040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3967..3986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4121..4915
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4175..4194)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4785..4804
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5091..5212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5128..5147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5182..5201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5261..5277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5261..5277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5274..5296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5285..5301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5309..5315,5316..5333,5334..5339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5354..5375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5492..5509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5663..6251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5743..5762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6422..6550,1..663,664..732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
       61 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
      361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
      421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
      481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
      541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
      601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      841 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
      901 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
      961 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     1021 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     1081 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     1141 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1201 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1261 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1321 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1381 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1441 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1501 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1561 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     1621 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1681 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     1741 catagaagac accgggaccg atccagcctc cggactgagc tccttaaggt taactctaga
     1801 gaattcggat ccatgacgcg ggagacttgc tacttcctca acgccaccat cctattcctg
     1861 ttccgggagt tgcgggacac cgcgctgacc cgccgctggg tcaccaagaa gatcaaggtg
     1921 gagttcgagg agctgctgca gaccaagacg gccgggcgcc tgctggaggg gctgagcctg
     1981 cgggacgtgt tcctgggcga gacggtgccc ttcatcaaga ccatccggct cgtgcggcca
     2041 gtcgtgccct cggccaccgg ggagcccgat ggccctgaag gggaggcgct gcccgccgcc
     2101 tgccccgagg agctggcctt cgaggcggag gtggagtaca acgggggctt ccacctggcc
     2161 atcgacgtgg acctggtctt cggcaagtcc gcctacttgt ttgtcaagct gtcccgcgtg
     2221 gtgggaaggc tgcgcttggt ctttacgcgc gtgcccttca cccactggtt cttctccttc
     2281 gtggaagacc cgctgatcga cttcgaggtg cgctcccagt ttgaagggcg gcccatgccc
     2341 cagctcacct ccatcatcgt caaccagctc aagaagatca tcaagcgcaa gcacacccta
     2401 ccgaattaca agatcaggtt taagccgttt tttccatacc agaccttgca aggatttgaa
     2461 gaagatgaag agcatatcca tatacaacaa tgggcactta ctgaaggccg tcttaaagtt
     2521 acgttgttag aatgtagcag gttactcatt tttggatcct atgacagaga ggcaaatgtt
     2581 cattgtacac ttgagttaag cagtagtgtt tgggaagaaa aacagaggag ttctattaag
     2641 acggttgaat taataaaagg aaatttacaa agtgttggac ttacacttcg tcttgtccag
     2701 tcaactgatg ggtatgctgg gcacgtcatc attgaaactg tggctccaaa ctcgcctgct
     2761 gcaattgcag atcttcagcg gggagatcga cttatcgcca ttggaggtgt gaaaatcaca
     2821 tcaacactgc aagtgttgaa gcttatcaag caggctggtg accgagtcct ggtgtactat
     2881 gaaaggcctg ttctcgagcc atggaagctt atggaacaaa aactcatctc agaagaggat
     2941 ctggatatct aactgactga accggtggtc caaacggggg aggctaactg aaacacggaa
     3001 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     3061 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     3121 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     3181 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     3241 ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3481 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3601 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3661 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3721 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3781 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3961 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4021 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4081 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4141 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4201 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4261 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4321 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4381 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4441 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4501 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4561 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4621 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4681 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4741 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4801 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4861 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4921 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     4981 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5041 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     5101 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5161 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5221 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5281 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6061 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6541 ccagtgctgc
//
