LOCUS       sequence-479449-       11051 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Expresses hyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Xue N, Liu X, Zhang D, Wu Y, Zhong Y, Wang J, Fan W, Jiang H, Zhu B,
            Ge X, Gonzalez RVL, Chen L, Zhang S, She P, Zhong Z, Sun J, Chen X, 
            Wang L, Gu Z, Zhu P, Liu M, Li D, Zhong TP, Zhang X
  TITLE     Improving adenine and dual base editors through introduction of 
            TadA-8e and Rad51DBD.
  JOURNAL   Nat Commun. 2023 Mar 3;14(1):1224. doi: 10.1038/s41467-023-36887-1.
  PUBMED    36869044
REFERENCE   2  (bases 1 to 11051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-3"; volume: "14(1):1224. doi"; pages: " 
            10.1038/s41467-023-36887-1"
COMMENT     Sequence Label: hyA&C-BEmax
FEATURES             Location/Qualifiers
     source          1..11051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2083..2106,2107..2109,2110..6183)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2083..6183
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6184..6231
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6241..6294
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6295..6543
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6574..6822
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6865..6885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6895..6951
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6952..6954,6955..6957,6958..7668)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6952..6954,6955..6957,6958..7668)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7258..7277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7605..7626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7681..7701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7724..7741)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7730..7954
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8038..8060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8049..8065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8073..8079,8080..8097,8098..8103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8118..8139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8256..8273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8427..9015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8507..8526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9186..9977,9978..10046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9809..9828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10047..10151)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10230..10249)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10441..10689
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10441..10461
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10612..10631
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10708..10783
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        join(10803..11051,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgacag cctgctgatg
      481 aaccggagaa agttcctgta tcagtttaag aatgtgaggt gggcaaaggg caggcgcgag
      541 acctacctgt gctatgtggt gaagcggaga gatagcgcca catctttcag cctggacttt
      601 ggctacctgc ggaacaagaa tggctgccac gtggagctgc tgtttctgag atacatctcc
      661 gactgggatc tggacccagg caggtgttat cgcgtgacct ggttcacatc ctggtctccc
      721 tgctacgatt gtgcccggca cgtggccgac tttctgagag gcaaccctaa tctgtctctg
      781 aggatcttca ccgcccgcct gtatttttgc gaggatagga aggccgagcc agagggactg
      841 aggcgcctgc acagggccgg cgtgcagatc gccatcatga cattcaagga ctacttttat
      901 tgttggaaca ccttcgtgga gaatcacgag cggacattta aggcctggga gggactgcac
      961 gagaactccg tgcggctgtc tagacagctg cggagaatcc tgctgccaga ggccgccgcc
     1021 aaggaagctg ccgccaagga ggccgccgcc aagtctgagg tggagttttc ccacgagtac
     1081 tggatgagac atgccctgac cctggccaag agggcacggg atgagaggga ggtgcctgtg
     1141 ggagccgtgc tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc
     1201 ctgcacgacc caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg
     1261 cagaactaca gactgattga cgccaccctg tacgtgacat tcgagccttg cgtgatgtgc
     1321 gccggcgcca tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaactcaaaa
     1381 agaggcgccg caggctccct gatgaacgtg ctgaactacc ccggcatgaa tcaccgcgtc
     1441 gaaattaccg agggaatcct ggcagatgaa tgtgccgccc tgctgtgcga tttctatcgg
     1501 atgcctagac aggtgttcaa tgctcagaag aaggcccaga gctccatcaa ctccggagga
     1561 tctagcggag gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa
     1621 agcagcgggg gcagcagcgg ggggtcagca atgcagatgc agcttgaagc aaatgcagat
     1681 acttcagtgg aagaagaaag ctttggccca caacccattt cacggttaga gcagtgtggc
     1741 ataaatgcca acgatgtgaa gaaattggaa gaagctggat tccatactgt ggaggctgtt
     1801 gcctatgcgc caaagaagga gctaataaat attaagggaa ttagtgaagc caaagctgat
     1861 aaaattctgg ctgaggcagc taaattagtt ccaatgggtt tcaccactgc aactgaattc
     1921 caccaaaggc ggtcagagat catacagatt actactggct ccaaagagct tgacaaacta
     1981 cttcaatccg gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag
     2041 agcgcaacac ctgaaagcag cgggggcagc agcggggggt cagacaagaa gtacagcatc
     2101 ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     2161 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     2221 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     2281 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     2341 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     2401 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     2461 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     2521 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     2581 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     2641 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
     2701 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     2761 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
     2821 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     2881 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     2941 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     3001 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     3061 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     3121 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     3181 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     3241 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     3301 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     3361 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     3421 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     3481 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     3541 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     3601 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     3661 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     3721 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     3781 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     3841 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     3901 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     3961 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     4021 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     4081 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     4141 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     4201 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     4261 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     4321 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     4381 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     4441 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     4501 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     4561 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
     4621 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     4681 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     4741 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     4801 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     4861 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     4921 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     4981 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     5041 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     5101 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     5161 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     5221 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     5281 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     5341 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     5401 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     5461 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     5521 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
     5581 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     5641 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     5701 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     5761 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     5821 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     5881 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     5941 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     6001 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     6061 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     6121 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc
     6181 gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatccgga
     6241 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaactaat
     6301 ctgagcgaca tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg
     6361 atgctgcctg aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg
     6421 cacaccgcct acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc
     6481 gagtataagc cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg
     6541 ctgagcggag gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag
     6601 gagacaggca agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa
     6661 gaagtgatcg gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt
     6721 accgacgaaa atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg
     6781 gtcatccagg attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga
     6841 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtcggaag cggagctact
     6901 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc tatggtgagc
     6961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     7021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     7081 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     7141 accctgacct atggagtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     7201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     7261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     7321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     7381 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     7441 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     7501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     7561 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     7621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc tggtggttct
     7681 cccaagaaga aaaggaaagt ctaagtttaa acccgctgat cagcctcgac tgtgccttct
     7741 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7801 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7861 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat
     7921 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7981 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gatgtttcct
     8041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8101 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
    10201 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
    10261 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    10321 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    10381 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtg gatccctgca gttaattaaa
    10441 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
    10501 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
    10561 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
    10621 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
    10681 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
    10741 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt atgcatggta
    10801 ccgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    10861 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    10921 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    10981 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    11041 tcaagtgtat c
//
