LOCUS       sequence-479452-        8932 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC PDZD8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC PDZD8
FEATURES             Location/Qualifiers
     source          1..8932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     495..514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(733..837)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(912..931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1103..1482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1483..1686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1637..1657
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1683..1707
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5296..5325
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5371..5390)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5415..5463
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5665..6093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5752..5771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5962..5983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6102..6122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6107..6436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6287..6422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6349..6368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6503..7297
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6557..6576)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7167..7186
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7473..7594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7510..7529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7564..7583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7643..7659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7643..7659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7656..7678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7667..7683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7691..7697,7698..7715,7716..7721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7736..7757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7874..7891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8045..8633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8125..8144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8804..8932,1..663,664..732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
       61 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
      361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
      421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
      481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
      541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
      601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      841 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
      901 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
      961 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     1021 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     1081 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     1141 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1201 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1261 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1321 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1381 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1441 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1501 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1561 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     1621 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1681 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     1741 catagaagac accgggaccg atccagcctc cggactgagc tccttaaggt taactctaga
     1801 gaattcggat ccatggggct gctgctcatg atcctggcgt cggccgtgct gggttccttc
     1861 ctcacgctcc tcgcccagtt cttcctgctg taccgcagac agcccgagcc gccggcggac
     1921 gaggccgccc gcgcgggcga gggcttccgc tacatcaagc cagtgccggg cctgctccta
     1981 agggagtacc tttatggcgg cggccgggat gaggagccct ccggagcggc ccctgagggc
     2041 ggcgcgaccc ccaccgcggc ccccgagacc cccgccccgc cgacgcggga gacttgctac
     2101 ttcctcaacg ccaccatcct attcctgttc cgggagttgc gggacaccgc gctgacccgc
     2161 cgctgggtca ccaagaagat caaggtggag ttcgaggagc tgctgcagac caagacggcc
     2221 gggcgcctgc tggaggggct gagcctgcgg gacgtgttcc tgggcgagac ggtgcccttc
     2281 atcaagacca tccggctcgt gcggccagtc gtgccctcgg ccaccgggga gcccgatggc
     2341 cctgaagggg aggcgctgcc cgccgcctgc cccgaggagc tggccttcga ggcggaggtg
     2401 gagtacaacg ggggcttcca cctggccatc gacgtggacc tggtcttcgg caagtccgcc
     2461 tacttgtttg tcaagctgtc ccgcgtggtg ggaaggctgc gcttggtctt tacgcgcgtg
     2521 cccttcaccc actggttctt ctccttcgtg gaagacccgc tgatcgactt cgaggtgcgc
     2581 tcccagtttg aagggcggcc catgccccag ctcacctcca tcatcgtcaa ccagctcaag
     2641 aagatcatca agcgcaagca caccctaccg aattacaaga tcaggtttaa gccgtttttt
     2701 ccataccaga ccttgcaagg atttgaagaa gatgaagagc atatccatat acaacaatgg
     2761 gcacttactg aaggccgtct taaagttacg ttgttagaat gtagcaggtt actcattttt
     2821 ggatcctatg acagagaggc aaatgttcat tgtacacttg agttaagcag tagtgtttgg
     2881 gaagaaaaac agaggagttc tattaagacg gttgaattaa taaaaggaaa tttacaaagt
     2941 gttggactta cacttcgtct tgtccagtca actgatgggt atgctgggca cgtcatcatt
     3001 gaaactgtgg ctccaaactc gcctgctgca attgcagatc ttcagcgggg agatcgactt
     3061 atcgccattg gaggtgtgaa aatcacatca acactgcaag tgttgaagct tatcaagcag
     3121 gctggtgacc gagtcctggt gtactatgaa aggcctgttg gccagagtaa tcaaggtgca
     3181 gtgctgcaag ataactttgg ccagttggaa gaaaactttt tgtcaagctc atgccaatcg
     3241 ggttatgaag aggaagctgc cgggttgaca gtagatactg aaagtagaga gctggattct
     3301 gaatttgaag acttggcaag tgatgtcaga gcacaaaatg agttcaaaga tgaggcacaa
     3361 tcattaagtc atagtcccaa acgtgttcca acaacacttt ctattaaacc ccttggagct
     3421 atatcaccag ttttaaaccg taaattagct gtaggaagtc acccactacc accgaaaatt
     3481 cagtccaaag atggaaataa acctccaccc ctaaaaactt ctgagataac agacccagca
     3541 caagtgtcaa aaccaaccca aggatctgct ttcaaaccac ctgtgccacc acgaccacaa
     3601 gcgaaagttc ctttgccttc cgccgatgct ccaaatcagg cagaaccaga tgttctcgtt
     3661 gaaaagccag agaaggtggt gccacctcct cttgtagata aatctgctga aaagcaagca
     3721 aaaaatgtgg atgccataga cgatgcagct gcacctaagc aatttttagc aaagcaagaa
     3781 gtggccaaag atgtcacttc agaaacttcc tgccctacta aggacagttc ggacgaccgt
     3841 caaacatggg aatcatcaga aattctttat cgtaataagc taggaaaatg gacaagaacc
     3901 agagcatcct gtttgtttga catagaagcc tgtcacaggt acttaaacat tgcattgtgg
     3961 tgcagggatc ctttcaagtt gggaggtctc atctgtttgg ggcatgttag tttaaaactt
     4021 gaagatgtgg ctttaggatg cctagctaca tcaaacacgg aatacctttc caaattgaga
     4081 ctggaagccc cctcacctaa ggctatagtc actagaaccg cactacgcaa tctgagtatg
     4141 caaaagggat tcaatgacaa attttgctat ggtgacatta ctattcactt caaatatttg
     4201 aaagaaggag aatcagacca ccatgtagtt actaacgtag aaaaagaaaa agaaccccat
     4261 ttggttgaag aagtttctgt tctccctaaa gaggagcaat ttgttggaca gatgggttta
     4321 acagaaaaca aacacagttt tcaggatact cagttccaga acccaacatg gtgtgactac
     4381 tgtaagaaaa aagtttggac taaagcagct tcccagtgta tgttttgtgc ttatgtttgc
     4441 cataaaaaat gtcaagaaaa gtgtctagct gagacttctg tttgtggagc aactgatagg
     4501 cgaatagaca ggacactgaa aaaccttagg ctggaaggac aggaaaccct cttaggcctg
     4561 cctcctcgtg ttgatgctga agctagcaag tcagtcaata aaacaacagg tttgacaagg
     4621 catattatca atactagttc tcgtttatta aatttgcgtc aagtctctaa aactcgcctt
     4681 tctgaaccag gaaccgatct cgtagaacct tcaccaaaac acacacccaa cacgtcagac
     4741 aacgaaggca gtgacacgga ggtctgtggt ccaaacagtc cttctaaacg gggaaacagc
     4801 acaggaataa agttagtgag aaaagagggt ggtctggatg acagtgtttt cattgcagtt
     4861 aaagaaattg gtcgtgatct gtacaggggc ttgcctacag aggaaaggat ccagaaacta
     4921 gagttcatgt tggataagct acagaatgaa attgatcagg agttggaaca caataattcc
     4981 cttgttagag aagaaaaaga gacaactgat acaaggaaaa aatcacttct ttctgctgcc
     5041 ttagctaaat caggtgaaag gctacaagct ctaacacttc ttatgattca ctacagagca
     5101 ggcattgaag atatagaaac tttagaaagt ctgtctttag accagcactc caaaaaaata
     5161 agcaagtaca cagatgatac agaagaagac cttgataatg aaataagcca actaatagac
     5221 tctcagccat tcagcagcat atcagatgac ttatttggcc catccgagtc tgtgctcgag
     5281 ccatggaagc ttatggaaca aaaactcatc tcagaagagg atctggatat ctaactgact
     5341 gaaccggtgg tccaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5401 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     5461 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     5521 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     5581 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca
     5641 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5701 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5761 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5821 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5881 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5941 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6001 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6061 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6121 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6181 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6241 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6301 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6361 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6421 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     6481 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     6541 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     6601 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     6661 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     6721 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     6781 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6841 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6901 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6961 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     7021 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     7081 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     7141 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     7201 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     7261 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa
     7321 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7381 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7441 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7501 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7561 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7621 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7681 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7741 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7801 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8461 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8521 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8581 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8641 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8701 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8761 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8821 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8881 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gc
//
