LOCUS       sequence-479457-        7720 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Used for transfection of cells for expression of C-MYC PDZD8 
            (91-839aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-MYC PDZD8(91-839aa)
FEATURES             Location/Qualifiers
     source          1..7720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     495..514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(733..837)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(912..931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1103..1482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1483..1686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1637..1657
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1683..1707
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4084..4113
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4159..4178)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4203..4251
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4453..4881
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4540..4559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4750..4771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4890..4910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4895..5224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5075..5210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5137..5156
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5291..6085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5345..5364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5955..5974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6261..6382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6298..6317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6352..6371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6431..6447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6431..6447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6444..6466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6455..6471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6479..6485,6486..6503,6504..6509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6524..6545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6662..6679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6833..7421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6913..6932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7592..7720,1..663,664..732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
       61 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
      121 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
      181 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
      241 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
      301 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
      361 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
      421 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
      481 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
      541 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
      601 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      661 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      721 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      781 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      841 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
      901 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
      961 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     1021 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     1081 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     1141 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1201 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1261 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     1321 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1381 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1441 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     1501 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     1561 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     1621 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1681 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     1741 catagaagac accgggaccg atccagcctc cggactgagc tccttaaggt taactctaga
     1801 gaattcggat ccatgacgcg ggagacttgc tacttcctca acgccaccat cctattcctg
     1861 ttccgggagt tgcgggacac cgcgctgacc cgccgctggg tcaccaagaa gatcaaggtg
     1921 gagttcgagg agctgctgca gaccaagacg gccgggcgcc tgctggaggg gctgagcctg
     1981 cgggacgtgt tcctgggcga gacggtgccc ttcatcaaga ccatccggct cgtgcggcca
     2041 gtcgtgccct cggccaccgg ggagcccgat ggccctgaag gggaggcgct gcccgccgcc
     2101 tgccccgagg agctggcctt cgaggcggag gtggagtaca acgggggctt ccacctggcc
     2161 atcgacgtgg acctggtctt cggcaagtcc gcctacttgt ttgtcaagct gtcccgcgtg
     2221 gtgggaaggc tgcgcttggt ctttacgcgc gtgcccttca cccactggtt cttctccttc
     2281 gtggaagacc cgctgatcga cttcgaggtg cgctcccagt ttgaagggcg gcccatgccc
     2341 cagctcacct ccatcatcgt caaccagctc aagaagatca tcaagcgcaa gcacacccta
     2401 ccgaattaca agatcaggtt taagccgttt tttccatacc agaccttgca aggatttgaa
     2461 gaagatgaag agcatatcca tatacaacaa tgggcactta ctgaaggccg tcttaaagtt
     2521 acgttgttag aatgtagcag gttactcatt tttggatcct atgacagaga ggcaaatgtt
     2581 cattgtacac ttgagttaag cagtagtgtt tgggaagaaa aacagaggag ttctattaag
     2641 acggttgaat taataaaagg aaatttacaa agtgttggac ttacacttcg tcttgtccag
     2701 tcaactgatg ggtatgctgg gcacgtcatc attgaaactg tggctccaaa ctcgcctgct
     2761 gcaattgcag atcttcagcg gggagatcga cttatcgcca ttggaggtgt gaaaatcaca
     2821 tcaacactgc aagtgttgaa gcttatcaag caggctggtg accgagtcct ggtgtactat
     2881 gaaaggcctg ttggccagag taatcaaggt gcagtgctgc aagataactt tggccagttg
     2941 gaagaaaact ttttgtcaag ctcatgccaa tcgggttatg aagaggaagc tgccgggttg
     3001 acagtagata ctgaaagtag agagctggat tctgaatttg aagacttggc aagtgatgtc
     3061 agagcacaaa atgagttcaa agatgaggca caatcattaa gtcatagtcc caaacgtgtt
     3121 ccaacaacac tttctattaa accccttgga gctatatcac cagttttaaa ccgtaaatta
     3181 gctgtaggaa gtcacccact accaccgaaa attcagtcca aagatggaaa taaacctcca
     3241 cccctaaaaa cttctgagat aacagaccca gcacaagtgt caaaaccaac ccaaggatct
     3301 gctttcaaac cacctgtgcc accacgacca caagcgaaag ttcctttgcc ttccgccgat
     3361 gctccaaatc aggcagaacc agatgttctc gttgaaaagc cagagaaggt ggtgccacct
     3421 cctcttgtag ataaatctgc tgaaaagcaa gcaaaaaatg tggatgccat agacgatgca
     3481 gctgcaccta agcaattttt agcaaagcaa gaagtggcca aagatgtcac ttcagaaact
     3541 tcctgcccta ctaaggacag ttcggacgac cgtcaaacat gggaatcatc agaaattctt
     3601 tatcgtaata agctaggaaa atggacaaga accagagcat cctgtttgtt tgacatagaa
     3661 gcctgtcaca ggtacttaaa cattgcattg tggtgcaggg atcctttcaa gttgggaggt
     3721 ctcatctgtt tggggcatgt tagtttaaaa cttgaagatg tggctttagg atgcctagct
     3781 acatcaaaca cggaatacct ttccaaattg agactggaag ccccctcacc taaggctata
     3841 gtcactagaa ccgcactacg caatctgagt atgcaaaagg gattcaatga caaattttgc
     3901 tatggtgaca ttactattca cttcaaatat ttgaaagaag gagaatcaga ccaccatgta
     3961 gttactaacg tagaaaaaga aaaagaaccc catttggttg aagaagtttc tgttctccct
     4021 aaagaggagc aatttgttgg acagatgggt ttaacagaaa acctcgagcc atggaagctt
     4081 atggaacaaa aactcatctc agaagaggat ctggatatct aactgactga accggtggtc
     4141 caaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4201 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     4261 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     4321 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     4381 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta
     4441 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4501 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4561 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4621 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4681 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4741 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4861 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5161 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5221 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5281 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5341 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5401 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5461 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5521 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5581 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5641 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5701 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5761 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5821 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5881 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5941 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6001 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6061 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
     6121 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6181 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6241 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6361 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6781 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6841 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6901 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6961 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7021 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7081 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7141 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7201 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7261 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7321 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7381 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7441 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7501 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc
//
