LOCUS       sequence-479459-        4071 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Rozario T, Quinn EB, Wang J, Davis RE, Newmark PA
  TITLE     Region-specific regulation of stem cell-driven regeneration in 
            tapeworms.
  JOURNAL   eLife. 2019 Sep 24;8. pii: 48958. doi: 10.7554/eLife.48958.
  PUBMED    31549962
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-09-24"; volume: "8. pii: 48958. doi"; pages: " 
            10.7554/eLife.48958"
COMMENT     Sequence Label: TR34_U3_hox-like
FEATURES             Location/Qualifiers
     source          1..4071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(548..1342)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(659..678)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1269..1288
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      complement(1749..1796)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1810..1828
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1832..1850
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2441..2452
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        complement(2698..2716)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2699..2716)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(2720..2738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2752..2799
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2811..2828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2982..3570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3062..3081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3741..4071,1..461,462..530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
       61 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      121 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      181 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      241 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      301 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      361 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      421 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      481 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      541 tttcaattca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
      601 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
      661 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
      721 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
      781 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
      841 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
      901 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
      961 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     1021 caaggtggga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     1081 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     1141 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
     1201 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     1261 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     1321 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
     1381 atcccctgcg ccatcagatc attggcggca agaaagccat ccagtttact ttgcagggct
     1441 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa
     1501 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc
     1561 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt
     1621 tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgaatttt
     1681 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     1741 cgacgcgtca aaaaacccct caagacccgt ttagaggccc caaggggtta tgctaggcgc
     1801 gccggatcct aatacgactc actataggga gaattaaccc tcactaaagg gagacctttt
     1861 gaactgatgg aggtggctgt aatttcggtg gttcgaaagg gttgataact gaaagttgag
     1921 agttattccc cgagggcgca aaatcattat gcacggagtc tttcccatca gactgctggc
     1981 caggactctt actaggatcc atcttcatac tggaagagaa atgaaacggc ggtgggtttc
     2041 caggataacc tcctgcccca ccactgttat atggtggtgg aagggcattt tgatgctgag
     2101 aaggtgctcc agaatccaag ctcgaaacag tgctaggaca tccgctgcgt gatccgtttc
     2161 cgccttctcc tccctggcca ccatgccctc catcatcatt accactcgca tcgtgtttac
     2221 tgtttgtgct gttcaaattt ggcgcacgag cttgaagttt cttgctcttc atgcgtcgat
     2281 tttggaacca cactttcacc tgacgttctg tgagatgaag cttgcaggag atttcccatc
     2341 gcttttgacg agtaatgtaa gttgctacaa ggtactcgtt ctcaagtacc atggtttgat
     2401 atcttgtata cggtttcctc ttttttctac ctcgaggcgt gtcatcctca tcgccattgc
     2461 ctggcatcat tgaagaaggc atgaagcatg gtggtggacc aaggcgattt gctggatcac
     2521 caggtgctgg aatcgggccc ataggtccag atccgtttcc taaagctaga gctgcagcag
     2581 cagcagcatg cacgctgttt gcgattctgg gagtctggaa aagacctggg ggtggtggag
     2641 gtccgccgtg tgattgtggt tgattgcctg ggtgtccgaa gtattgctaa ggtcacttct
     2701 atagtgtcac ctaaatctcc ctatagtgag tcgtattagg atccggcgcg cctagcataa
     2761 ccccttgggg cctctaaacg ggtcttgagg ggttttttga cgcgtgcttc ctcgctcact
     2821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3781 tgtctatttc gttcatccat agttgcctgg ctccccgtcg tgtagataac tacgatacgg
     3841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt g
//
