LOCUS       sequence-479465-       15393 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Express FLS2XL in planta under potato StUbi7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15393)
  TITLE     Introducing Pattern Recognition Receptors in Potato Confers Enhanced
            Resistance to Ralstonia Solanacearum but not a Vector Borne Pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pStUbi7::FLS2XL-EGFP
FEATURES             Location/Qualifiers
     source          1..15393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5225..5249
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    5383..5394
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(10760..10784)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(10789..10791,10792..10794,10795..11508)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10834..10855)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11095..11114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11442..11463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      11533..11785
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11818..11834)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11818..11834)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11831..11853)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11842..11858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11866..11872,11873..11890,11891..11896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11911..11932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(11966..12218)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12608..13402)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12719..12738)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13329..13348
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(13423..13606)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(13459..13638)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13812..13836
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14357..15148
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggctct
     5221 aatcacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcgcgc ccacccttca
     5281 ccccaatatt tgaaatctca attttatgat tttttaaatt cactttatat ccaagacaat
     5341 ttcacttcaa aaagttttat taaaatttac attagttttg ttgatgagga tgacaagatt
     5401 tggtcatcaa ttacatatac ccaaattgaa tagtaagcaa cttcaatgtt tttcataatg
     5461 ataatgacag acacaaaaac ccatttatta ttcacattga ttgagtttta tatgcaatat
     5521 agtaataata ataatatttc ttataaagca agaggtcaat ttttttttaa ttataccacg
     5581 tcactaaatt atatttgata atgtaaaaca attcaaattt tacttaaata tcatgaaata
     5641 aactattttt ataaccaaat tactaaattt ttccaataaa aaaaagtcat taagaagaca
     5701 taaaataaat ttgaggtaaa gagtgaagtc gactgacttt tttttttttt tatcataaga
     5761 aaataaatta ttaactttaa cctaataaaa cactaatata atttcatgga atctaatact
     5821 tacctcttag aaataagaaa aagtgtttct aatagaccct caatttacat taaatatttt
     5881 caatcaaatt ttaaataaca aatatcaata tgaggtcaat aacaatatca aaataatatg
     5941 aaaaaagagc aatacataat ataagggacg atttaagtgc gattatcaag gtagtattat
     6001 atcctaattt gctaatattt ggctcttata tttaaggtca tgttcatgat aaacttgaaa
     6061 tgcgctatat tagagcatat attaaaataa aaaaatacct aaaataaaat taagttattt
     6121 ttagtatata tttttttaca tgacctacat ttttctgggt ttttctaaag gagcgtgtaa
     6181 gtgtcgacct cattctccta attttcccca ccacataaaa attaaaaagg aaaggtagct
     6241 tttgcgtgtt gttttggtac actacacctc attattaccc gtgtcctcat ataattggtt
     6301 aaccctatga ggcggtttcg tcttgagtcg gccatgccat ctataaaatg aagcattctg
     6361 cacctcattt ttttcatctt ctatctgatt tctattataa tttctctcaa ttgccttcaa
     6421 atttctcttt aaggttagaa tcttctctat ttttggtttt tgtatgttta gattctcgaa
     6481 ttagctaatc aggcgctgtt atagcccttc cttttgagtc tctcctcggt tgtcttgatg
     6541 gaaaaggcct aacatttgag tttttttacg tctggtttga tggaaaaggc ctacaattgg
     6601 ccgttttccc cgttcgtttt gatgaaaaag cccctagttt gagatttttt ttctgtcgtt
     6661 cgttctaaag gtttaaaatt agagttttta catttgtttg atgaaaaagg ccttaaattt
     6721 gagtttttcc ggttgatttg atgaaaaagc cctagaattt gtgtttttcc gtcggtttga
     6781 ttctgaaggc ctaaaatttg agtttctccg gctgttttga tgaaaaagcc ctaaatttga
     6841 gtttctccgg ctgttttgat gaaaaagccc taaatttgaa gttttttccc cgtgttttag
     6901 attgtttagg ttttaattct cgaatcagct aatcagggag tgtgaaagcc ctaaattgaa
     6961 gtttttttcg ttgttctgat tgttgttttt atgaatttgc agatgcagat atttgtgaaa
     7021 actctcaccg gaaagactat caccctagag gtggaaagtt ctgatacaat cgacaacgtt
     7081 aaggctaaga tccaggataa ggaagggatt cccccggatc agcaaaggct tatcttcgcc
     7141 ggaaagcagt tggaggacgg acgtactcta gctgattaca acatccagaa ggagtctacc
     7201 ctccatttgg tgctccgtct acgtggaggt ggactcgaga tggagcccag aagtgttggt
     7261 ttaagagtag tggtagtttg ttctgttctg gttgtggtgg ttataagcat ggatccaagt
     7321 tttgaagtcg accatcaggc tttgaaggcc ttcaagagtt cagttgctga cgatccctct
     7381 ggagtgcttg cagattggag tgaagccaac catcattgca attggtctgg aatcacatgt
     7441 gacccttcat ccagtcgtgt catgtcaatt atactgatgg agaagcagct tgccggcgtg
     7501 atctcgccat tccttggaaa cctttccaaa ctccaggttc tggacttaac tttaaacaca
     7561 ttcactgggc aaattccacc tcaactgggg ctttgctccc agctttcaga actgattctt
     7621 taccaaaatt ctcttgccgg accaattcca caagaactag gaatcctagg gaatttacaa
     7681 tcattagatt tgggggctaa ctttttggaa ggaagcattc ctgaaagcat ttgtaactgc
     7741 actggcttgc tgaatctggg cattgataac aacaatctca gtggtgccat cccttccgac
     7801 attggaagat tagataatct tcaagtcttc acagggtaca gaaataattt ggttggttct
     7861 ataccagttt ccattggcac gttgggagct ttgcaggttc tagacctgag tacaaaccat
     7921 ttgtctggtg ttttacctcc agaaatgggg aacttgtcaa accttgaaac ccttcagttg
     7981 cttgaaaatc gacttcacgg gaaaatccca cctgaactag ggctctgtag gaagctcacc
     8041 actctgaatt tgtatggcaa tcagttcagt gggggcattc cttcagaact aggaaacttg
     8101 gttcatttga aagcgctgcg gttgtacaaa aataggttaa gttctacaat tccatcctcc
     8161 ttattccaat tgaaatcatt ggtccacttg ggaatatcag agaatgaatt gagtggaaca
     8221 atcccttttg aagttgggtc tctgagatca ttgcaagcac tgacgctgca gttaaataag
     8281 ttcactgggc aaatcccatc atccattaca aacttgacaa acttaacata tttgtctatg
     8341 gacttcaact tcttcacagg tgatattccg tccaacatcg gatcgcttta tcgtttgaag
     8401 aacctaaccc taaacaataa ccttctccag gggtccgttc catctagtat ctctaactgc
     8461 acacgtctag tcgtcttagg tctagcttat aatagaataa caggaagaat tccacaaggt
     8521 ttggggcggc ttgcaaatct tatttttcta tctttcggga agaaccaaat gtcgggcaac
     8581 attcctgatg atctcttcaa ttgctcaaat ctgattattc tagatttggc caagaacaat
     8641 ttcagtggag ttctgaaacc aggcattggc aagctctata atctgcatat ttttcaagct
     8701 cacaaaaatt ccttagtagg gcctatccca cctgagattg gtaatctaag ccaactgttc
     8761 tcattaaaac tacacttgaa tagtttgtcg ggcacagttc ctcctgaatt atccaagctt
     8821 tccctcctcc agggtctcta tctgaatgac aatgctctag aaggtgcact tcctgaggta
     8881 atttttgaac taaaacagct tagtgatctt gggttgggta acaaccggtt tgcaggaccc
     8941 attccacatg ctatctctaa gcttgagtcg cttttgtact tgaccctcca tggaaacctg
     9001 ttcaatggat ccattccaac gagcatggga cacctttctc ggctggcaac cttggatctc
     9061 tctcacaatc atctggtcgg gtcaattccg gggcctgtca ttgctggtat gaaaaacatg
     9121 caaatatatc tcaacttttc acacaatttc ctatcagggc ccattccaaa tgagcttggg
     9181 aaattggaga tggtgcagat cgtggatatg tcaaacaata atctatcagg gagcattcca
     9241 gcaactcttc aacgctgcaa aaacttgttc aatattgatc tgtcagtgaa ccaactttcc
     9301 ggtacaattc cagagaaggc atttgcggga atggatgtac ttacaagctt gaatctttca
     9361 agaaataatt tagatggtag actacctgga agcttagcga ttatgaaaaa tctaagctcc
     9421 ctggaccttt ctcagaacaa gttcaagggc atgatccccg agagttatgc aaatatttcc
     9481 acattgaggc atctgaatct ttctttcaat caactggaag gtcatgtccc agcgactgga
     9541 atattcaaaa atataggtgc atcttctctg gtgggaaacc caggtctttg tggaaccaaa
     9601 tttctcgggt catgcagcaa taagagccat ttagccggtt ctcatccgtt ctccaagaaa
     9661 gttctgttga ttctcggggt agttggatct ctcattgtat tgctgcttct cacattttct
     9721 gttataatct tctgtcggta cttcagaaag caaaaaacag ttgaaaatcc agaaccagag
     9781 tatgcttcag cattgacgct caagaggttc aatcaaaagg atttagaaat agctactggt
     9841 ttctttagtg aagaaaacat tattgggtcc agcagtttga gtaccgtata caagggcaga
     9901 atggaagatg ggaagattgt agccgtaaag aaattaaatt tgcaacagtt ctcattggag
     9961 tctgacaagt gcttcaatag agaagtgaag accctgagcc aactcaggca cagaaacttg
    10021 gtaaaggtac ttgggtatgc ctgggaaagt ggaaaaataa aggctcttgt tctggaatac
    10081 atggagaagg ggaacttgga cagcattata catgaaccag gggtggatcc atcaaggtgg
    10141 actttattgg agagaatcaa tgtttgtatt tccattgcaa gggggctggt ttacttgcat
    10201 tcagggtatg atttccccat cgtccactgt gacttgaagc cttcaaatat tctcttggat
    10261 ggagattggg aggcccatgt gagtgatttt ggaacagctc gaattctggg tgttcatctc
    10321 caggatggaa gcagtgtttg ctcatcatca gcatttgaag gcacaattgg atacttggca
    10381 ccagagttgg catacatgag ggagctcacg accaaagtgg atgtgttcag ctttggcatc
    10441 atagtgatgg agttcctgac aaaaagaagg ccaacaggac tgacagcaga ggatggcatg
    10501 ccattgacct tgcgtgagat ggtggatatg gcccttgcga gtgaattcaa aaggcttctt
    10561 caaatgatag acccttttct agcttcatct gcaactgaga aggcggggga agtattggaa
    10621 gaggtcctta aactggcctt gtcttgcacc tgcacagagc ctgaggatcg acctgacatg
    10681 aatgaggtgt tgtccagcct tttgaagcta ggagcaaagg ttgcttcatc aacagctact
    10741 agtaagggtg ggcgcgccga cccagctttc ttgtacaaag tggtgatcat ggtgagcaag
    10801 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
    10861 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
    10921 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
    10981 ttcacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
    11041 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    11101 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    11161 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    11221 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
    11281 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
    11341 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
    11401 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    11461 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtaaag cggcccgagc
    11521 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
    11581 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
    11641 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
    11701 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
    11761 gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatgtc
    11821 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    11881 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat
    11941 taggcgcgcc tatttctgaa ttcccgatct agtaacatag atgacaccgc gcgcgataat
    12001 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
    12061 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
    12121 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
    12181 aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggatcatccg ggtctgtggc
    12241 gggaactcca cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct
    12301 gggcagtcgc cgccgacgcc gttgatgtgg acgccgggcc cgatcatatt gtcgctcagg
    12361 atcgtggcgt tgtgcttgtc ggccgttgct gtcgtaatga tatcggcacc ttcgaccgcc
    12421 tgttccgcag agatcccgtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
    12481 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
    12541 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
    12601 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
    12661 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
    12721 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
    12781 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
    12841 gggtcacgac gagatcatcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
    12901 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
    12961 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
    13021 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
    13081 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
    13141 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
    13201 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
    13261 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
    13321 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
    13381 gcaatccatc ttgttcaatc atgcgaaacg atccagatcc ggtgcagatt atttggattg
    13441 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
    13501 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
    13561 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct
    13621 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
    13681 gggggtcata acgtgactcc cttaattctc cgctcatgat gaattcagaa ataaattcag
    13741 cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
    13801 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
    13861 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
    13921 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
    13981 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
    14041 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
    14101 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
    14161 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
    14221 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
    14281 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
    14341 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
    14401 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
    14461 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
    14521 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
    14581 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
    14641 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
    14701 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
    14761 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
    14821 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
    14881 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
    14941 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
    15001 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
    15061 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
    15121 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
    15181 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
    15241 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
    15301 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    15361 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
