LOCUS       sequence-479476-        9491 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  GFP expressing shRNA targeting Drp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Salazar MPR, Kolanukuduru S, Ramirez V, Lyu B, Sejourne G, Sesaki H,
            Yu G, Eroglu C
  TITLE     Mitochondrial fission controls astrocyte morphogenesis and 
            organization in the cortex.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 22:2024.10.22.619706. doi: 
            10.1101/2024.10.22.619706.
  PUBMED    39484572
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 22:2024.10.22.619706. doi: 
            10.1101/2024.10.22.619706."
COMMENT     Sequence Label: pLKO.1-shDrp1-GFP
FEATURES             Location/Qualifiers
     source          1..9491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    101..122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(137..142,143..160,161..167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    175..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     180..202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     199..215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     199..215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     234..254
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        236..254
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        282..508
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             509..689
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    736..861
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1354..1587
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1772..1816
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2114..2362
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2114..2134
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2285..2304
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2459..2576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2943..3246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3567..4583
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4507..4529
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4591..4610
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4660..4669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4666..4668,4669..4671,4672..5385)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4666..4668,4669..4671,4672..5382)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4711..4732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4972..4991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5319..5340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5405..5438)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5494..6082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5547..5567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5965..5976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6085..6101)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6086..6102)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6291..6524
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6596..6717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6633..6652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6687..6706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6757..6892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6912..6931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6913..6931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6941..6958)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6941..6957)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6950..6972)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7099..7554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7186..7205)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7396..7417
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7580..7684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7685..7753,7754..8545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7903..7922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8716..9304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9205..9224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9458..9475
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      241 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
      301 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
      361 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
      421 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
      481 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
      541 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      601 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      661 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      721 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
      781 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
      841 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
      901 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
      961 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1021 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1081 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1141 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1201 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1261 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1321 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1381 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1441 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1501 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1561 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1621 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1681 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1741 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1801 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1861 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     1921 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     1981 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2041 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc
     2101 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     2161 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     2221 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     2281 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     2341 cttgtggaaa ggacgaaaca ccggctataa tgcatgcact atttactcga gtaaatagtg
     2401 catgcattat agtttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     2461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2581 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
     2641 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     2701 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     2761 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     2821 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     2881 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     2941 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     3001 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     3061 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3121 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3181 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     3241 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     3301 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     3361 ggggggggcg cgcgccaggg gggggggggg gggggggggg gggggggggg ggggcgcggg
     3421 gggggggggg ggggggcgag ggggggggcg gggcgaggcg gagaggtgcg gcggcagcca
     3481 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     3541 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
     3601 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     3661 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     3721 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     3781 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     3841 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     3901 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     3961 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     4021 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     4081 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     4141 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     4201 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     4261 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     4321 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     4381 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     4441 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     4501 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     4561 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     4621 attttggcaa agaattcgcg gccgctagcg ctaccggtcg ccaccatggt gagcaagggc
     4681 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4741 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4801 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4861 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4921 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4981 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5041 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5101 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5161 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5221 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5281 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5341 accgccgccg ggatcactct cggcatggac gagctgtaca agtaggaatt cgtcgaggga
     5401 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc
     5461 cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     5521 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5581 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5641 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5701 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5761 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5821 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5881 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     5941 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6001 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6061 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     6121 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6181 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6241 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6301 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6361 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6421 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6481 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6541 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6601 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6661 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6721 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6781 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6841 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     6901 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     6961 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7021 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7081 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7141 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7201 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7261 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7321 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7381 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7441 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7501 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7561 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7621 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7681 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7741 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7801 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7861 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7921 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7981 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8041 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8101 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8161 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8221 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8281 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8341 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8401 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8461 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8521 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8581 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8821 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9421 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9481 ggaagagcgc c
//
