LOCUS       sequence-479512-        7199 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  5' AAVLINK plasmid for CDC42BPB expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Zhonghua Lu Lab
  TITLE     Zhonghua Lu AAV Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' CDC42BPB
FEATURES             Location/Qualifiers
     source          1..7199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   4462..4602
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4462..4591
                     /label=AAV2 ITR
     rep_origin      4677..5132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4764..4783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4974..4995
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5149..5168)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5268..5290
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5328..5346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5414..5518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5519..5587,5588..6379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5737..5756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6550..7138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7039..7058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatcttc ggccaaggtg cggctcaaga agctggagca
      781 gctgctcctg gacgggccct ggcgcaacga gagcgccctg agcgtggaaa cgctgctcga
      841 cgtgctcgtc tgcctgtaca ccgagtgcag ccactcggcc ctgcgccgcg acaagtacgt
      901 ggccgagttc ctcgagtggg ctaaaccatt tacacagctg gtgaaagaaa tgcagcttca
      961 tcgagaagac tttgaaataa ttaaagtaat tggaagaggt gcttttggtg aggttgctgt
     1021 tgtcaaaatg aagaatactg aacgaattta tgcaatgaaa atcctcaaca agtgggagat
     1081 gctgaaaaga gcagagaccg cgtgcttccg agaggagcgc gatgtgctgg tgaacggcga
     1141 ctgccagtgg atcaccgcgc tgcactacgc ctttcaggac gagaaccacc tgtacttagt
     1201 catggattac tatgtgggtg gtgatttact gaccctgctc agcaaatttg aagacaagct
     1261 tccggaagat atggcgaggt tctacattgg tgaaatggtg ctggccattg actccatcca
     1321 tcagcttcat tacgtgcaca gagacattaa acctgacaat gtccttttgg acgtgaatgg
     1381 tcatatccgc ctggctgact ttggatcatg tttgaagatg aatgatgatg gcactgtgca
     1441 gtcctccgtg gccgtgggca cacctgacta catctcgccg gagatcctgc aggcgatgga
     1501 ggacggcatg ggcaaatacg ggcctgagtg tgactggtgg tctctgggtg tctgcatgta
     1561 tgagatgctc tatggagaaa cgccgtttta tgcggagtca ctcgtggaga cctatgggaa
     1621 gatcatgaac catgaagagc gattccagtt cccatcccat gtcacggatg tatctgaaga
     1681 agcgaaggac ctcatccaga gactgatctg cagtagagaa cgccggctgg ggcagaatgg
     1741 aatagaggat ttcaaaaagc atgcgttttt tgaaggtcta aattgggaaa atatacgaaa
     1801 cctagaagca ccttatattc ctgatgtgag cagtccctct gacacatcca acttcgacgt
     1861 ggatgacgac gtgctgagaa acacggaaat attacctcct ggttctcaca caggcttttc
     1921 tggattacat ttgccattca ttggttttac attcacaacg gaaagctgtt tttctgatcg
     1981 aggctctctg aagagcataa tgcagtccaa cacattaacc aaagatgagg atgtgcagcg
     2041 ggacctggag cacagcctgc agatggaagc ttacgagagg aggattcgga ggctggaaca
     2101 ggagaagctg gagctgagca ggaagctgca agagtccacc cagaccgtgc agtccctcca
     2161 cggctcatct cgggccctca gcaattcaaa ccgagataaa gaaatcaaaa agctaaatga
     2221 agaaatcgaa cgcttgaaga ataaaatagc agattcaaac aggctggagc gacagcttga
     2281 ggacacagtg gcgcttcgcc aagagcgtga ggactccacg cagcggctgc gggggctgga
     2341 gaagcagcac cgcgtggtcc ggcaggagaa ggaggagctg cacaagcaac tggttgaagc
     2401 ctcagagcgg ttgaaatccc aggccaagga actcaaagat gcccatcagc agcgaaagct
     2461 ggccctgcag gagttctcgg agctgaacga gcgcatggca gagctccgtg cccagaagca
     2521 gaaggtgtcc cggcagctgc gagacaagga ggaggagatg gaggtggcca cgcagaaggt
     2581 ggacgccatg cggcaggaaa tgcggagagc tgagaagctc aggaaagagc tggaagctca
     2641 gcttgatgat gctgttgctg aggcctccaa ggagcgcaag cttcgtgagc acagcgagaa
     2701 cttctgcaag caaatggaaa gcgagctgga ggccctcaag gtgaagcaag gaggccgggg
     2761 agcgggtgcc accttagagc accagcaaga gatttccaaa atcaaatccg agctggagaa
     2821 gaaagtctta ttttatgaag aggaattggt cagacgtgag gcctcccatg tgctagaagt
     2881 gaaaaatgtg aagaaggagg tgcatgattc agaaagccac cagctggccc tgcagaaaga
     2941 aatcttgatg ttaaaagata agttagaaaa gtcaaagcga gaacggcata acgagatgga
     3001 ggaggcagta ggtacaataa aagataaata cgaacgagaa agagcgatgc tgtttgatga
     3061 aaacaagaag ctaactgctg aaaatgaaaa gctctgttcc tttgtggata aactcacagc
     3121 tcaaaataga cagctggagg atgagctgca ggatctggca gccaagaagg agtcagtggc
     3181 ccactgggaa gctcagattg cggaaatcat tcagtgggtc agtgacgaga aagatgcccg
     3241 gggttacctt caagctcttg cttccaagat gaccgaagag ctcgaggctt tgaggagttc
     3301 tagtctgggg tcaagaacac tggacccgct gtggaaggtg cgccgcagcc agaagctgga
     3361 catgtccgcg cggctggagc tgcagtcggc cctggaggcg gagatccggg ccaagcagct
     3421 tgtccaggag gagctcagga aggtcaagga cgccaacctc accttggaaa gcaaactaaa
     3481 ggattccgaa gccaaaaaca gagaattatt agaagaaatg gaaattttga agaaaaagat
     3541 ggaagaaaaa ttcagagcag atactgggct caaacttcca gattttcagg attccatttt
     3601 tgagtatttc aacactgctc ctcttgcaca tgacctgaca tttagaacca gctcagctag
     3661 tgagcaagaa acacaagctc cgaagccaga agcgtccccg tcgatgtctg tggctgcatc
     3721 agagcagcag gagccaaaag ctcaccagtt cagcatcaag tccttctcca gccctactca
     3781 gtgcagccac tgcacctccc tgatggttgg gctgatccgg cagggctacg cctgcgaggt
     3841 gtgttccttt gcttgccacg tgtcctgcaa agacggtgcc ccccaggtgt gcccaatacc
     3901 tcccgagcag tccaagaggc ctctgggcgt ggacgtgcag cgaggcatcg gaacagccta
     3961 caaaggccat gtcaaggtcc caaagcccac gggggtgaag aagggatggc agcgcgcata
     4021 tgcagtcgtc tgtgactgca agctcttcct gtatgatctg cctgaaggaa aatccaccca
     4081 gcctggtgtc attgcgagcc aagtcttgga tctcagagat gacgagtttt ccgtgagctc
     4141 agtcctggcc tcagatgtca ttcatgctac acgccgagat attccatgta tattcagggt
     4201 gacggcctct ctcttaggtg caccttctaa gaccagctcg ctgctcattc tgacagaaaa
     4261 tgagaatgaa aagaggaagt gggttgggat tctagaagga ctccagtcca tccttcataa
     4321 aaaccggctg aggaatcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
     4381 agctattatt cgtataggat actttatacg aagttattaa gttatggaga ccggtgtggc
     4441 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4501 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4561 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4621 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4681 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4741 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4801 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4861 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     4921 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4981 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     5041 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5101 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     5161 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5221 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5281 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5341 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5761 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6061 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6241 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6301 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
