LOCUS       sequence-479774-        5988 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  mouse Serbp1 fused to meGFP for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5988)
  TITLE     Stemness factor Mex3a times translation and protein trafficking to 
            ensure robust differentiation of olfactory sensory neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV-Serbp1-meGFP
FEATURES             Location/Qualifiers
     source          1..5988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        286..489
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     440..460
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1861..2574
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(1900..1921)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2161..2180)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2508..2529
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2697..2818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2734..2753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2788..2807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2825..3280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2962..2983)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3174..3193
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3307..3411
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3413..3770
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3413..3604
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3436..3456)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3621..3756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3805..4599
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3805..4599
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3859..3878)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4469..4488
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4787..4806)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4831..4878
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5207..5795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5696..5715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(5970..5988,1..285)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
       61 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      121 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      181 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      241 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      301 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      361 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      421 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      481 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
      541 tcaagcttcg aattcatgcc tgggcaccta caggaaggct tcggctgcgt ggtcaccaac
      601 cgattcgacc agctatttga cgacgaatcg gaccctttcg aggtactgaa ggcagcagag
      661 aacaagaaaa aagaagccgg cgggggcggc gttgggggcc ccggggccaa gagcgcggct
      721 caggccgcgg cccagaccaa ctccaacgcg gcgggcaaac agttgcgtaa agagtcccag
      781 aaagaccgca agaacccgct gccccccagc gtcggcgtgg ccgacaaaaa ggaggagacg
      841 cagccgccgg tggcgcttaa gaaagaagga ataaggcgag ttggaagaag acccgatcaa
      901 caactacagg gtgatgggaa actaattgat aggagagcag aaaggcgacc accccgtgaa
      961 agaagatttg aaaagccact tgaagaaaaa ggtgaaggag gtgaattttc agttgataga
     1021 ccgattatcg aacggcctat ccgaggccga ggtggtcttg gaaggggtcg aggaggccgt
     1081 ggacgtggaa tgggccgagg cgatggattt gattctcgtg gcaagcgtga atttgatagg
     1141 catagtggaa gtgatagatc ttctttttca cattacagtg gcctgaagca tgaggacaaa
     1201 cgcggaggta gcggctctca caactgggga actgtcaaag atgaattaac agagtcaccc
     1261 aaatacattc agaaacaaat atcttataat tgcagtgatt tggatcaatc aaatgtgact
     1321 gaggaaacac ctgaaggtga agagcaccct gtggcagata ctgaaaataa ggagaacgaa
     1381 gttgaagagg ttaaggaaga gggtccaaaa gagatgactc tggatgagtg gaaagctatt
     1441 caaaataaag accgagcaaa agtagaattt aatatccgaa aaccaaatga aggcgccgat
     1501 ggacaatgga aaaagggatt tgttctgcat aaatcaaaaa gtgaagaggc tcatgctgaa
     1561 gattcagtta tggaccatca tttccggaag ccagcaaatg atataacatc tcaactggag
     1621 atcaattttg gagacttagg ccgcccagga cgtggtggca gaggaggacg tggtgggcgt
     1681 gggcgtggtg gacgtccaaa ccgtggcagc aggactgata agtcaagtgc ttctgctcct
     1741 gatgtagatg acccagaggc cttcccagct ctggccgaat tcggaggcgg aggttctggg
     1801 ggaggaggta gtggcggtgg tggttcagga ggcggcggaa gcggtggagg tggaagcggt
     1861 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1921 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1981 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2041 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2101 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2161 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2221 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2281 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2341 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2401 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2461 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2521 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     2581 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     2641 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     2701 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2761 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     2821 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2881 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2941 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3001 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3061 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3121 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3181 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3241 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3301 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3361 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     3421 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3481 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3541 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3601 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3721 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     3781 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3841 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3901 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3961 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     4021 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4081 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4141 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4201 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4261 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4321 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4381 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4441 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4501 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4561 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4621 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4681 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4741 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     4801 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4861 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     4921 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     4981 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5041 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5881 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
     5941 ttagttcata gcccatatat ggagttccgc gttacataac ttacggta
//
