LOCUS       sequence-479815-        5221 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Express SRSF1-LgBiT Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SRSF1-LgBiT Fusion Vector
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..753
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     primer_bind     complement(2164..2182)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    2219..2340
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2230..2249)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2284..2303
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2585..2942
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2585..2776
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2608..2628)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2793..2928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2957..3004
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3036..3830
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3090..3109)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3700..3719
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3894..3942
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4181..4769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4261..4280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4885..5168
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 aaatgagtct tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg
       61 gggggagggg gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt
      121 ggccacgggc acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga
      181 ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc
      241 cccagagtgt gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg
      301 acgggctcgc tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat
      361 ccgcacgact gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct
      421 gacgcggagc gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc
      481 acccggaggc gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt
      541 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
      601 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaagtcga acacgcagat
      661 gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
      721 gaacaccgag cgaccctgca gcgacccgct taaaagcttg gcaatccggt actgttggta
      781 aagccaccgc tagcgatcgc catgtcggga ggtggtgtga ttcgtggccc cgcagggaac
      841 aacgattgcc gcatctacgt gggtaactta cctccagaca tccgaaccaa ggacattgag
      901 gacgtgttct acaaatacgg cgctatccgc gacatcgacc tcaagaatcg ccgcggggga
      961 ccgcccttcg ccttcgttga gttcgaggac ccgcgagacg cggaagacgc ggtgtatggt
     1021 cgcgacggct atgattacga tgggtaccgt ctgcgggtgg agtttcctcg aagcggccgt
     1081 ggaacaggcc gaggcggcgg cgggggtgga ggtggcggag ctccccgagg tcgctatggc
     1141 cccccatcca ggcggtctga aaacagagtg gttgtctctg gactgcctcc aagtggaagt
     1201 tggcaggatt taaaggatca catgcgtgaa gcaggtgatg tatgttatgc tgatgtttac
     1261 cgagatggca ctggtgtcgt ggagtttgta cggaaagaag atatgaccta tgcagttcga
     1321 aaactggata acactaagtt tagatctcat gagggagaaa ctgcctacat ccgggttaaa
     1381 gttgatgggc ccagaagtcc aagttatgga agatctcgat ctcgaagccg tagtcgtagc
     1441 agaagccgta gcagaagcaa cagcaggagt cgcagttact ccccaaggag aagcagagga
     1501 tcaccacgct attctccccg tcatagcaga tctcgctctc gtacagtttc tcaaggcagt
     1561 tcaggtggtg gcgggagcgg aggtggaggc tcgagcggtg tcttcacact cgaagatttc
     1621 gttggggact gggaacagac agccgcctac aacctggacc aagtccttga acagggaggt
     1681 gtgtccagtt tgctgcagaa tctcgccgtg tccgtaactc cgatccaaag gattgtccgg
     1741 agcggtgaaa atgccctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc
     1801 gccgaccaaa tggcccagat cgaagaggtg tttaaggtgg tgtaccctgt ggatgatcat
     1861 cactttaagg tgatcctgcc ctatggcaca ctggtaatcg acggggttac gccgaacatg
     1921 ctgaactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact
     1981 gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat cacccccgac
     2041 ggctccatgc tgttccgagt aaccatcaac agctaaggcc gcgactctag agtcgacctg
     2101 caggcatgca agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
     2161 ccaccgctga gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata
     2221 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2281 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2341 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     2401 ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct
     2461 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2521 cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta
     2581 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2641 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     2701 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2761 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2821 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2881 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2941 aaaagcttaa ttaactgttg acaattaatc atcggcatag tatatcggca tagtataata
     3001 cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat ggattgcacg
     3061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3181 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3541 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     3601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     3841 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa
     3901 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     3961 tcctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     4021 gtcagcaccg tttctgcgga ctggctttct acccggtatc agctcactca aaggcggtaa
     4081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4741 agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg
     4801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4861 tcttcaccta gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg
     4921 ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc
     4981 ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc
     5041 agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
     5101 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
     5161 gccatcgaga taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc
     5221 t
//
