LOCUS       sequence-479855-        9029 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Donor Vector to integrate mClover3 C-terminal into the human PER1 
            Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hPER1-mClover3-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             join(1180..1182,1183..1893)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1900..1965
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1975..2008)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2091..2111)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2096..2426
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2277..2412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      2476..2485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2482..2484,2485..2487,2488..3198)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2788..2807)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3214..3270
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3277..3672
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3684..3805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3721..3740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3775..3794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3867..3900)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4921..5124
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5075..5095
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5121..5145
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5219..5293,5294..6490)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(6808..6824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6808..6824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6821..6843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6832..6848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6856..6862,6863..6880,6881..6886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6901..6922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7039..7056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7210..7798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7290..7309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7969..8760,8761..8829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8592..8611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8830..8934)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9002..9020
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta aggtttaaac tctgaacccc ttcttgggca gggacatgac
      241 ctctgtgctg aagcaggatc gggagcggct ccgagccatg cagaagcagc agcctcggtt
      301 ttctgaggac cagcggcggg aactgggtgc tgtgcactcc tgggtccgga agggccaact
      361 gcctcgggct cttgatgtga tggtgagaga agcctgggac ggggagaaaa aagaattgag
      421 ctcaagttca agggggagaa aaaagaattg agctcaagtt caagggggag aaaaaagaat
      481 tgagctcaag ttcaagggat cgaggccaag agctgatctc cttgatgtcc ttggatcatt
      541 aattctgaag aatgttgatt ccactaaatt tgctgtggat tatagaatat taagccgcgt
      601 gagtctttgc agaacttttc acagcctatc ctatgctaat atgcattgtg actgtcctgt
      661 aacggcatct gggtagaggg cacaaggcac tgtccaacct tgttggaccg caggtgcatc
      721 tgtgtggact ggtgcttctt gggagtacat ttcgggaagc acagtgggct gggggtggga
      781 agctgcgctg gcaggttagc agtgagaacc ctgtctgact ctctcatgtc catttctctc
      841 accaaggcct gtgtggactg tgggagcagc acccaagatc ctggtcaccc tgatgaccca
      901 ctcttctcag agctggatgg actggggctg gagcccatgg aagagggtgg aggcgagcag
      961 ggcagcagcg gtggcggcag tggtgaggga gagggctgcg aggaggccca aggcggggcc
     1021 aaggcttcaa gctctcagga cttggctatg gaggaggagg aagaaggcag gagctcatcc
     1081 agtccagcct tacctacagc aggaaactgc accagcaccg gtggcggtgg tagcggcggt
     1141 ggtggctctg gtggcggtgg ttcaacgcgt ggtggcagcg tgagcaaggg cgaggagctg
     1201 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1261 agcgtccgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     1321 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     1381 gtggcctgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1441 atgcccgaag gctacgtcca ggagcgcacc atctctttca aggacgacgg tacctacaag
     1501 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1561 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     1621 cactacgtct atatcacggc cgacaagcag aagaactgca tcaaggctaa cttcaagatc
     1681 cgccacaacg ttgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1741 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagccatca gtccaagctg
     1801 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1861 gggattacac atggcatgga cgagctgtac aagactagtg actacaaaga ccatgacggt
     1921 gattataaag atcatgacat cgattacaag gatgacgatg acaagttaga tctaataact
     1981 tcgtatagca tacattatac gaagttattt tgaataaaat atctttattt tcattacatc
     2041 tgtgtgttgg ttttttgtgt tgagctgatt taacaaaaat ttaacgctgt ggaatgtgtg
     2101 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     2161 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2221 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2281 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2341 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     2401 tttttggagg cctaggcttt tgcaaaaagc tattgtatat ccattttcgg atctgatcag
     2461 cactgagcag gtaccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2521 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2581 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2641 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc
     2701 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2761 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2821 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2881 gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa cgtctatatc
     2941 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     3001 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3061 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     3121 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3181 atggacgagc tgtacaagga attcggaagc ggagctacta acttcagcct gctgaagcag
     3241 gctggagacg tggaggagaa ccctggacct cacgtggcca agcctttgtc tcaagaagaa
     3301 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     3361 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     3421 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     3481 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     3541 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     3601 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     3661 tgggagggct aactcgagac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3781 tgtccaaact catcaatgta tcttatcatg tctgtataga agttcctatt ccgaagttcc
     3841 tattcttcaa atagtatagg aacttcataa cttcgtatag catacattat acgaagttat
     3901 ttgattaatt aagcgatcgc actccattct gggaccatct cgaggagtcg atgagaggct
     3961 gtcttctcct atgtcccaat tctcagaact cagatgtggc tagaccaacc agtgggacac
     4021 tgccccagct tctcccacca tagggggccg gacccccatc accagcctag gatccagggg
     4081 ctgcctctgg cctcttaggg agcagagagc agaactccgc agcccagccc agaggagtgt
     4141 cacctcccac ctttggagag gaatccttcc ctcccctgga caaagttgct gacaagctgc
     4201 tgaagtggcc tctccatatt ccagctgagc ctgaatctga ctcttgaggg ttggggctgc
     4261 acttatttat tgcggggaga cagctctctc tcccacctcc tccccagatg ggaggagagc
     4321 ctgaggccca agcaggaccc gggggttcca gcccctagct gctctggagt gggggaggtt
     4381 ggtggaccat ggagtccctg gtgctgcccc tcaggtggga cccaggcgtt ctcagctgta
     4441 ccctctgccg atggcatttg tgtttttgat atttgtgtct gttactactt ttttaataca
     4501 aaaagataaa aacgcccagg actttgtgga atgaagtttg gggttggggt gaggggagca
     4561 tgtcaagggg agatgtggcc ctgcttttgg gaaggttgta gtgagggtgt acagggccat
     4621 ctcctgacga cccccattcc tcttttcccc catcctgtcc aggtgagggc cctgctgagc
     4681 ccgctacgtc cacgtccagc tcagactctg ctgtcccctc tagcatggcc tcagctgggt
     4741 ttgccctggg aaggagagct gagaggtgcg tgtggccaag ggcttgggta agctccagcc
     4801 cgggtcacgg gtggccaagg cacctgcagg tcaattccct ggcattatgc ccagtacatg
     4861 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4921 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4981 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5041 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     5101 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     5161 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctggg gatccaccat
     5221 gaaccgggga gtccctttta ggcacttgct tctggtgctg caactggcgc tcctcccagc
     5281 agccactcag ggaaagaaag tggtgctggg caaaaaaggg gatacagtgg aactgacctg
     5341 tacagcttcc cagaagaaga gcatacaatt ccactggaaa aactccaacc agataaagat
     5401 tctgggaaat cagggctcct tcttaactaa aggtccatcc aagctgaatg atcgcgctga
     5461 ctcaagaaga agcctttggg accaaggaaa cttccccctg atcatcaaga atcttaagat
     5521 agaagactca gatacttaca tctgtgaagt ggaggaccag aaggaggagg tgcaattgct
     5581 agtgttcgga ttgactgcca actctgacac ccacctgctt caggggcaga gcctgaccct
     5641 gaccttggag agcccccctg gtagtagccc ctcagtgcaa tgtaggagtc caaggggtaa
     5701 aaacatacag ggggggaaga ccctctccgt gtctcagctg gagctccagg atagtggcac
     5761 ctggacatgc actgtcttgc agaaccagaa gaaggtggag ttcaaaatag acatcgtggt
     5821 gctagctttc cagaaggcct ccagcatagt ctataagaaa gagggggaac aggtggagtt
     5881 ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc tgtggtggca
     5941 ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga acaaggaagt
     6001 gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc tcccgctcca
     6061 cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca ccctggccct
     6121 tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga gagccactca
     6181 gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc tgatgctgag
     6241 cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg tgtgggtgct
     6301 gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg tcctgctgga
     6361 atccaacatc aaggttctgc ccacatggtc gaccccggtg cagccaatgg ccctgattgt
     6421 gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct tctgtgtcag
     6481 gtgccggcac tgaaggcgac ctgcggatcc cacgtcacta ttgtatactc tatattatac
     6541 tctatgttat actctgtaat cctactcaat aaacgtgtca cgcctgtgaa accgtactaa
     6601 gtctcccgtg tcttcttatc accatcaggt gacatcctcg cccaggctgt caatcatgcc
     6661 ggtatcgatt ccagtagcac cggccccacg ctgacaaccc actcttgcag cgttagcagc
     6721 gcccctctta acaagccgac ccccaccagc gtcgcggtta ctaacactcc tctccccgac
     6781 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6901 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6961 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7021 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7081 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7141 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7201 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7261 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7321 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7381 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7441 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7501 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7561 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7621 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7681 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7741 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7801 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7861 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7921 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7981 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8041 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8101 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8161 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8221 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8281 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8341 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8401 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8461 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8521 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8581 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8641 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8701 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8761 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8821 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8881 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8941 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9001 aaataggcgt atcacgaggc cctttcgtc
//
