LOCUS       sequence-479862-        9131 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Donor Vector to integrate mClover3 C-terminal into the human CRY2 
            Locus to express it as a fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pDB2-hCRY2-mClover3-CD4-bla
FEATURES             Location/Qualifiers
     source          1..9131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             join(1185..1187,1188..1898)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1905..1970
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(1980..2013)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2096..2116)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2101..2431
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2282..2417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2344..2363
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2481..2490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2487..2489,2490..2492,2493..3203)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2793..2812)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3219..3275
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3282..3677
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3689..3810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3726..3745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3780..3799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3872..3905)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5023..5226
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5177..5197
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5223..5247
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5321..5395,5396..6592)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(6910..6926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6910..6926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6923..6945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6934..6950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6958..6964,6965..6982,6983..6988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7003..7024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7141..7158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7312..7900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7392..7411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8071..8862,8863..8931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8694..8713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8932..9036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9104..9122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta aggtttaaac aggaaccgaa gggaggtcag tgtagcggca
      241 tgtcccagtg aggagctgga atgagatgag gacaagatac agcatgagca gcatgtggca
      301 ggcctgatgt ccgagacgag gactaggatt cagccttcag tgcactgaag gatggtcagt
      361 agggcagggc atgattggac ttaggtctga aaaagctccc tggcctgctg ggtggagatt
      421 ggacagtgta ggccggagcg ggcacaggga ggccagagag gaggcagcac agacagggtg
      481 gtggccgcaa ggagaaagac tggacagatt cgctgcattc ggagcctctt cccttcttgg
      541 ggatgaagat gagtaacatg agatgggaag aacacgggtt tttctagttg gcccagctag
      601 gctgcctcca tgggcagcat gactcctcca caggggccct gctctttgct gcttggggag
      661 gctgattcag ggttgcagag ggaatgcctc accttcaaag caggacagca ggcaggccaa
      721 atcagtctct ggccacagcc catgccattt ggggcccaaa agctggtcct gctaccagca
      781 ggcctgcttg gtcatgtcct ccttggacat ttccaggcaa agtcactgct gaaagcaagg
      841 ccgaggggca ggtctgagga gcagcatgct ggcattccat tgataagtgt gctccctgtc
      901 ccctaaagag ctgctgctgc atagtgtggg atcattttgg ctgcatgcac agtctgtgtg
      961 accctttgac acgcttccct acaggcccaa gaccactacc cagtggccca gcatccccca
     1021 aacgcaagct ggaagcagcc gaggaaccac ctggtgaaga actcagcaaa cgggcccggg
     1081 tggcagagtt gccaacccca gagctgccga gcaaggatgc caccggtggc ggtggtagcg
     1141 gcggtggtgg ctctggtggc ggtggttcaa cgcgtggtgg cagcgtgagc aagggcgagg
     1201 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1261 agttcagcgt ccgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt
     1321 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     1381 acggcgtggc ctgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1441 ccgccatgcc cgaaggctac gtccaggagc gcaccatctc tttcaaggac gacggtacct
     1501 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1561 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca
     1621 acagccacta cgtctatatc acggccgaca agcagaagaa ctgcatcaag gctaacttca
     1681 agatccgcca caacgttgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1741 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc catcagtcca
     1801 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1861 ccgccgggat tacacatggc atggacgagc tgtacaagac tagtgactac aaagaccatg
     1921 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag ttagatctaa
     1981 taacttcgta tagcatacat tatacgaagt tattttgaat aaaatatctt tattttcatt
     2041 acatctgtgt gttggttttt tgtgttgagc tgatttaaca aaaatttaac gctgtggaat
     2101 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag
     2161 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2221 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2341 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     2401 aggctttttt ggaggcctag gcttttgcaa aaagctattg tatatccatt ttcggatctg
     2461 atcagcactg agcaggtacc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     2521 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2581 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     2641 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct
     2701 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2761 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2821 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2881 aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatcagc gacaacgtct
     2941 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     3001 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3061 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     3121 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3181 tcggcatgga cgagctgtac aaggaattcg gaagcggagc tactaacttc agcctgctga
     3241 agcaggctgg agacgtggag gagaaccctg gacctcacgt ggccaagcct ttgtctcaag
     3301 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     3361 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     3421 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     3481 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     3541 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     3601 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     3661 atgtgtggga gggctaactc gagaccccaa cttgtttatt gcagcttata atggttacaa
     3721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3781 tggtttgtcc aaactcatca atgtatctta tcatgtctgt atagaagttc ctattccgaa
     3841 gttcctattc ttcaaatagt ataggaactt cataacttcg tatagcatac attatacgaa
     3901 gttatttgat taattaagcg atcgcggatg cctgagagtg agtgacagca gcctagattc
     3961 aacctcacga acgaagttgg gagtgggggg gcctactgcc ctgccagctg caggttgaca
     4021 catagcacac tagatgaaaa tctggtggga ccacccaatg ctcagccact aaagctttac
     4081 cattttacag tggcagccgg gttccatcct ggcttaggga atgagtactt tctggttgat
     4141 atctgtgtga cctttaccat tgggccggct atggtggctc atgtatgtaa tcccagcact
     4201 ttgggaggcc aaggtgggag gatcgcttga ggccaggagt acaagaccaa cctggccaaa
     4261 ccaacatagt gagactccat ctctaaaaac ataataataa taataataat aatctggtgg
     4321 gactgcatga cttttaggat ttcagctcaa aaatcccacc attctgactt ttatcagaga
     4381 tttaaaagtc aagccctttt gtattttttt gccagctaag ggaaaaaggg aggcatgggg
     4441 gctgaggggt ggcgctttct acctattaaa atgagtcaca ctcaagagaa agaaagctga
     4501 gacctgcgtg tgaagcagcg gggaaaaggc gcattgcagg gtctgaggct gctccaggcc
     4561 ccctgctttc cgacaggctg tgttctatag gccagccagt ggagggcagc actgaccttg
     4621 agaagcctgg acagagggct gtgctgtgtc ttacttggcc cagccctgcc tgaggaccag
     4681 tagacccagt ctgtcatcat ctttgccttt tgcggcctct cctcccttct cacccatcct
     4741 gtggcctcag ctctgcacct ccctggcagc gctcatcttg gaagcctcct gaatgtcatc
     4801 aaaacactgg cttctccttt cccaagggag gaagggcctt ttatgccact cttggggaaa
     4861 tgtaaattcc tgtcccctct gagtcatcat ctcaagctgt tccttatgca ccatgtcttc
     4921 gtcacctgca ggtcaattcc ctggcattat gcccagtaca tgaccttatg ggactttcct
     4981 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     5041 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5101 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     5161 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5221 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     5281 catagaagac accgggaccg atccagcctg gggatccacc atgaaccggg gagtcccttt
     5341 taggcacttg cttctggtgc tgcaactggc gctcctccca gcagccactc agggaaagaa
     5401 agtggtgctg ggcaaaaaag gggatacagt ggaactgacc tgtacagctt cccagaagaa
     5461 gagcatacaa ttccactgga aaaactccaa ccagataaag attctgggaa atcagggctc
     5521 cttcttaact aaaggtccat ccaagctgaa tgatcgcgct gactcaagaa gaagcctttg
     5581 ggaccaagga aacttccccc tgatcatcaa gaatcttaag atagaagact cagatactta
     5641 catctgtgaa gtggaggacc agaaggagga ggtgcaattg ctagtgttcg gattgactgc
     5701 caactctgac acccacctgc ttcaggggca gagcctgacc ctgaccttgg agagcccccc
     5761 tggtagtagc ccctcagtgc aatgtaggag tccaaggggt aaaaacatac agggggggaa
     5821 gaccctctcc gtgtctcagc tggagctcca ggatagtggc acctggacat gcactgtctt
     5881 gcagaaccag aagaaggtgg agttcaaaat agacatcgtg gtgctagctt tccagaaggc
     5941 ctccagcata gtctataaga aagaggggga acaggtggag ttctccttcc cactcgcctt
     6001 tacagttgaa aagctgacgg gcagtggcga gctgtggtgg caggcggaga gggcttcctc
     6061 ctccaagtct tggatcacct ttgacctgaa gaacaaggaa gtgtctgtaa aacgggttac
     6121 ccaggaccct aagctccaga tgggcaagaa gctcccgctc cacctcaccc tgccccaggc
     6181 cttgcctcag tatgctggct ctggaaacct caccctggcc cttgaagcga aaacaggaaa
     6241 gttgcatcag gaagtgaacc tggtggtgat gagagccact cagctccaga aaaatttgac
     6301 ctgtgaggtg tggggaccca cctcccctaa gctgatgctg agcttgaaac tggagaacaa
     6361 ggaggcaaag gtctcgaagc gggagaaggc ggtgtgggtg ctgaaccctg aggcggggat
     6421 gtggcagtgt ctgctgagtg actcgggaca ggtcctgctg gaatccaaca tcaaggttct
     6481 gcccacatgg tcgaccccgg tgcagccaat ggccctgatt gtgctggggg gcgtcgccgg
     6541 cctcctgctt ttcattgggc taggcatctt cttctgtgtc aggtgccggc actgaaggcg
     6601 acctgcggat cccacgtcac tattgtatac tctatattat actctatgtt atactctgta
     6661 atcctactca ataaacgtgt cacgcctgtg aaaccgtact aagtctcccg tgtcttctta
     6721 tcaccatcag gtgacatcct cgcccaggct gtcaatcatg ccggtatcga ttccagtagc
     6781 accggcccca cgctgacaac ccactcttgc agcgttagca gcgcccctct taacaagccg
     6841 acccccacca gcgtcgcggt tactaacact cctctccccg acctgcagcc caagcttggc
     6901 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6961 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7021 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7081 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7141 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7201 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7261 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7321 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7381 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7441 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7501 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7561 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7621 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7681 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7741 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7801 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7861 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7921 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7981 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8041 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8101 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8161 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8221 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8281 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8341 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8401 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8461 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8521 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8581 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8641 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8701 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8761 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8821 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8881 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8941 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9001 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9061 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9121 gccctttcgt c
//
