LOCUS       sequence-479871-        9472 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Expresses TurboID-Gab2 fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Kramer MH, Richardson SN, Li Y, Yin T, Helton NM, George DR, Cai M, 
            Ramakrishnan SM, Katerndahl CDS, Miller CA, Ley TJ
  TITLE     Overexpression of the signaling-coordinator GAB2 can play an 
            important role in Acute Myeloid Leukemia progression
  JOURNAL   J Clin Invest. 2025.
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2025."
COMMENT     Sequence Label: TurboID-Gab2-MSCV-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(213..1004,1005..1073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1074..1178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1246..1264
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1302..1324)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1424..1443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1637..1659
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1787..1806
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2006..2522
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2586..2927
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2994..3410
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3340..3362
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3378..3394
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3447..4406
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    6470..7037
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6487..7039
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6631..6648)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6858..6877
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7051..7053,7054..7056,7057..7770)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7357..7376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7704..7725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7872..8386
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8548..8564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8572..8578,8579..8596,8597..8602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8617..8638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8755..8772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8926..9472,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9006..9025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     1261 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     1321 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     1381 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     1441 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     1501 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     1561 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     1621 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     1681 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     1741 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     1801 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     1861 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     1921 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     1981 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2041 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     2101 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2161 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     2221 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     2281 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     2341 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     2401 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
     2461 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
     2521 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
     2581 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     2641 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     2701 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     2761 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     2821 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     2881 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     2941 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     3001 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     3061 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     3121 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     3181 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     3241 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     3301 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     3361 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
     3421 ctctcgaggt taaccaattc gccaccatga aagacaatac tgtgcctctg aagctgatcg
     3481 ctctcctggc taatggcgag ttccatagtg gcgaacagct gggagaaacc ctgggcatgt
     3541 ccagggccgc tatcaacaag cacattcaga ctctgcgcga ctggggcgtg gacgtgttca
     3601 ccgtgcccgg aaagggctac tctctgcccg agcctatccc gctgctgaac gctaaacaga
     3661 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     3721 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     3781 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     3841 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     3901 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     3961 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     4021 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     4081 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     4141 tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt
     4201 tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca
     4261 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     4321 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     4381 aaatcagtct gcggtctgcc gaaaagggtg gaggtggttc tggcggaggc ggctctggag
     4441 gaggaggaag tggatccatg agcggcggcg gcggcgacga cgtggtgtgt accggctggc
     4501 tgaggaaatc gcctcccgag aagaagttga ggcgctatgc ctggaagaaa cgctggttta
     4561 tacttcggag tggccgaatg agtggagatc cagatgttct ggaatactac aagaatgagc
     4621 actccaagaa acccctgcgg atcatcaacc tgaacttgtg tgagcaggtg gatgcaggcc
     4681 tgaccttcaa caagaaagag ctgcaggata gttttgtgtt tgatatcaag accagcgagc
     4741 gcacatttta cctggtggct gagacagagg ctgacatgaa taagtgggtc cagagcatct
     4801 gccagatctg cggcttcaat caggctgaag agagcacaga ctccctgagg aacctttctt
     4861 cagccagtca tggtccccgc tcttctccag ctgagttcag ctccagtcag cacctgctcc
     4921 gagaacggaa gtcctcagcc ccttcacact ctagccagcc tactttattc acgtttgagc
     4981 cccctgtgtc aagccacatg cagcctacct tgtccaccag tgcacctcag gagtatctct
     5041 acttgcacca gtgcataagc agaaggacag aaaatgcaag gagtgccagc ttctctcagg
     5101 gcacccggca gaagagtgat acagctgtgc aaaaacttgc ccagagcaat ggacactgta
     5161 tcaacggcgt cggaggtcaa gtccatggct tctatagcct tcccaagcca agccgacaca
     5221 atacagaatt caaagacagt acttatgatc tcccacggag cctggcttcc catggccaca
     5281 ccaagagcag cctcacaggg tctgagactg ataacgagga tgtgtacacc ttcaagatgc
     5341 ccagcaacac cctgtgtcgg gaacttggag acctccttgt ggacaatatg gatgtcccaa
     5401 ccactcctct ctcagcctac cagatcccta gaacattcac actggacaag aaccacaatg
     5461 ccatgacagt ggccactcct ggagattcag ccatagctcc cccaccccgg ccacccaagc
     5521 caagtcaggc agaaacacct caatggggca gcattcagca aagacctcca atcagcgaaa
     5581 atagcagatc tgtagctgct actatcccca ggcgcaatac cctccctgca atggacaaca
     5641 gccgactcca tcgagcttct tcctgtgaga cctacgagta cccggcacga ggcagtgggg
     5701 aaagtgccag ctggtctgct gaacctccag gaaagactgc cgtaggtcga tcaaatagtg
     5761 ccagctctga tgacaactac gtgcccatga acccaggttc ttctaccctg ctggctatgg
     5821 aacgaccagg ggacaactcc cagagtgtct acatccccat gagcccagga ccccatcact
     5881 ttgacccact tggctacccg tccacagccc ttcctattca cagaggcccc agccgaggaa
     5941 gtgaaatcca gccacccccg gtcaaccgaa acctcaagcc tgacagaaaa gcaaagccaa
     6001 caccccttga cctgagaaac aacactgtca tcgatgagct gcccttcaag tcacctgtca
     6061 ccaagtcttg gtccaggatc aacagccaca cctttaactc cagttcctcc cagtactgcc
     6121 gtcctatctc cacacagagc atcaccagca cagactcggg agacagtgag gagaactatg
     6181 tccctatgca aaacccagta tctgcatccc ctgttcccag tggcactaac agcccagctc
     6241 caaagaagag tactggcagt gtggattatc tcgccctgga cttccagccg ggctccccaa
     6301 gccctcaccg caagccatcc acatcatctg tcacatcaga tgagaaggta gactatgtcc
     6361 aagtggataa agagaagacc caggccctgc agaacaccat gcaggagtgg acagatgtgc
     6421 ggcagtcctc cgaaccttcc aagggtgcca agctgtaggg ttaacgaatt ccgccccccc
     6481 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6541 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6601 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6661 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     6721 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     6781 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     6841 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     6901 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     6961 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     7021 gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc
     7081 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     7141 tctggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     7201 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     7261 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7321 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7381 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7441 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7501 gtctatatca tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     7561 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7621 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     7681 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7741 actctcggca tggacgagct gtacaagtaa tgaattaatt aagaattatc aagcttatcg
     7801 ataccgtcga cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga
     7861 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     7921 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac
     7981 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     8041 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
     8101 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     8161 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     8221 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
     8281 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     8341 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct
     8401 tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat
     8461 tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg
     8521 cagaaagagc tggggagaat tgtgaaattg ttatccgctc acaattccac acaacatacg
     8581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt tt
//
