LOCUS       sequence-479872-        9442 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Expresses Gab2-TurboID fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Kramer MH, Richardson SN, Li Y, Yin T, Helton NM, George DR, Cai M, 
            Ramakrishnan SM, Katerndahl CDS, Miller CA, Ley TJ
  TITLE     Overexpression of the signaling-coordinator GAB2 can play an 
            important role in Acute Myeloid Leukemia progression
  JOURNAL   J Clin Invest. 2025.
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2025."
COMMENT     Sequence Label: Gab2-TurboID-MSCV-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(213..1004,1005..1073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1074..1178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1246..1264
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1302..1324)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1424..1443
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1637..1659
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1787..1806
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2006..2522
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2586..2927
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2994..3410
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3340..3362
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3378..3394
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             5473..6432
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    6440..7007
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6457..7009
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6601..6618)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6828..6847
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7021..7023,7024..7026,7027..7740)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7327..7346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7674..7695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7842..8356
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8518..8534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8542..8548,8549..8566,8567..8572))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8587..8608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8725..8742)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8896..9442,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8976..8995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     1261 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     1321 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     1381 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     1441 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     1501 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     1561 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     1621 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     1681 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     1741 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     1801 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     1861 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     1921 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     1981 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2041 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     2101 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2161 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     2221 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     2281 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     2341 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     2401 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
     2461 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
     2521 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
     2581 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     2641 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     2701 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     2761 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     2821 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     2881 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     2941 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     3001 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     3061 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     3121 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     3181 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     3241 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     3301 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     3361 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
     3421 ctcatgagcg gcggcggcgg cgacgacgtg gtgtgtaccg gctggctgag gaaatcgcct
     3481 cccgagaaga agttgaggcg ctatgcctgg aagaaacgct ggtttatact tcggagtggc
     3541 cgaatgagtg gagatccaga tgttctggaa tactacaaga atgagcactc caagaaaccc
     3601 ctgcggatca tcaacctgaa cttgtgtgag caggtggatg caggcctgac cttcaacaag
     3661 aaagagctgc aggatagttt tgtgtttgat atcaagacca gcgagcgcac attttacctg
     3721 gtggctgaga cagaggctga catgaataag tgggtccaga gcatctgcca gatctgcggc
     3781 ttcaatcagg ctgaagagag cacagactcc ctgaggaacc tttcttcagc cagtcatggt
     3841 ccccgctctt ctccagctga gttcagctcc agtcagcacc tgctccgaga acggaagtcc
     3901 tcagcccctt cacactctag ccagcctact ttattcacgt ttgagccccc tgtgtcaagc
     3961 cacatgcagc ctaccttgtc caccagtgca cctcaggagt atctctactt gcaccagtgc
     4021 ataagcagaa ggacagaaaa tgcaaggagt gccagcttct ctcagggcac ccggcagaag
     4081 agtgatacag ctgtgcaaaa acttgcccag agcaatggac actgtatcaa cggcgtcgga
     4141 ggtcaagtcc atggcttcta tagccttccc aagccaagcc gacacaatac agaattcaaa
     4201 gacagtactt atgatctccc acggagcctg gcttcccatg gccacaccaa gagcagcctc
     4261 acagggtctg agactgataa cgaggatgtg tacaccttca agatgcccag caacaccctg
     4321 tgtcgggaac ttggagacct ccttgtggac aatatggatg tcccaaccac tcctctctca
     4381 gcctaccaga tccctagaac attcacactg gacaagaacc acaatgccat gacagtggcc
     4441 actcctggag attcagccat agctccccca ccccggccac ccaagccaag tcaggcagaa
     4501 acacctcaat ggggcagcat tcagcaaaga cctccaatca gcgaaaatag cagatctgta
     4561 gctgctacta tccccaggcg caataccctc cctgcaatgg acaacagccg actccatcga
     4621 gcttcttcct gtgagaccta cgagtacccg gcacgaggca gtggggaaag tgccagctgg
     4681 tctgctgaac ctccaggaaa gactgccgta ggtcgatcaa atagtgccag ctctgatgac
     4741 aactacgtgc ccatgaaccc aggttcttct accctgctgg ctatggaacg accaggggac
     4801 aactcccaga gtgtctacat ccccatgagc ccaggacccc atcactttga cccacttggc
     4861 tacccgtcca cagcccttcc tattcacaga ggccccagcc gaggaagtga aatccagcca
     4921 cccccggtca accgaaacct caagcctgac agaaaagcaa agccaacacc ccttgacctg
     4981 agaaacaaca ctgtcatcga tgagctgccc ttcaagtcac ctgtcaccaa gtcttggtcc
     5041 aggatcaaca gccacacctt taactccagt tcctcccagt actgccgtcc tatctccaca
     5101 cagagcatca ccagcacaga ctcgggagac agtgaggaga actatgtccc tatgcaaaac
     5161 ccagtatctg catcccctgt tcccagtggc actaacagcc cagctccaaa gaagagtact
     5221 ggcagtgtgg attatctcgc cctggacttc cagccgggct ccccaagccc tcaccgcaag
     5281 ccatccacat catctgtcac atcagatgag aaggtagact atgtccaagt ggataaagag
     5341 aagacccagg ccctgcagaa caccatgcag gagtggacag atgtgcggca gtcctccgaa
     5401 ccttccaagg gtgccaagct gggatccggt ggaggtggtt ctggcggagg cggctctgga
     5461 ggaggaggaa gtatgaaaga caatactgtg cctctgaagc tgatcgctct cctggctaat
     5521 ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag ggccgctatc
     5581 aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt gcccggaaag
     5641 ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct gggacagctg
     5701 gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat
     5761 cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga
     5821 ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt
     5881 tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg
     5941 gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg
     6001 tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat
     6061 gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc
     6121 gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact
     6181 ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc
     6241 ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag acccgtgaag
     6301 ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc
     6361 ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg
     6421 tctgccgaaa agtagcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     6481 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     6541 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     6601 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     6661 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     6721 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     6781 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     6841 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     6901 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     6961 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     7021 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     7081 ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac
     7141 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     7201 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     7261 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     7321 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     7381 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     7441 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     7501 ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     7561 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     7621 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7681 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     7741 tgaattaatt aagaattatc aagcttatcg ataccgtcga cctgcagcca agcttatcga
     7801 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     7861 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     7921 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     7981 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     8041 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     8101 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     8161 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     8221 cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     8281 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     8341 agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag
     8401 gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa
     8461 tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
     8521 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8821 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9301 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9421 cgctggtagc ggtggttttt tt
//
