LOCUS       sequence-479884-       10046 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Express CRAF-BRAF Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoBRET Assay Vector, BiBRET CRAF-BRAF
FEATURES             Location/Qualifiers
     source          1..10046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    61..182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(72..91)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(297..330)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        340..387
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             419..1213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(473..492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1083..1102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1451..1470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2085..2133
                     /note="synthetic polyadenylation signal"
     CDS             complement(2157..2555)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      2552..2561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2586..2943)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2600..2735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2752..2943
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2900..2920
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    3022..3103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3280..3792)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     regulatory      6104..6113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6146..6164)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6147..6164)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        6284..6587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6588..6791
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6742..6762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6927..7059
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     7042..7061
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     7103..7122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7103..7121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9100..9120
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             9136..10020
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga
       61 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      121 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      181 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
      241 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagaat tctaatataa
      301 cttcgtatag catacattat acgaagttat ggatccactg ttgacaatta atcatcggca
      361 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
      421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     2101 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag
     2161 ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca
     2221 tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac
     2281 cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg
     2341 ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca
     2401 gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg
     2461 gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg
     2521 agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg
     2581 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2641 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2701 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2761 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2821 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2881 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     2941 cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgcaa
     3001 gacgcgttaa ttaagacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     3181 ctacaaatgt ggtatggctg attatgatcc gcggccgcaa tacgtcgacg ttatcagctg
     3241 acttcgtacg agagcctagg tgtttctaga gtcgcggcct tacgccagaa tgcgttcgca
     3301 cagccgccag ccggtcactc cgttgatggt tactcggaac agcagggagc cgtcggggtt
     3361 gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg gtccctgtta cagtgatctt
     3421 tttgccgtcg aacacggcga tgccttcata cggccgtccg aaatagtcga tcatgttcgg
     3481 cgtaaccccg tcgattacca gtgtgccata gtgcaggatc accttaaagt gatgatcatc
     3541 cacagggtac accaccttaa aaattttttc gatctggccc atttggtcgc cgctcagacc
     3601 ttcatacggg atgatgacat ggatgtcgat cttcagccca ttttcaccgc tcaggacaat
     3661 cctttggatc ggagttacgg acaccccgag attctgaaac aaactggaca cacctccctg
     3721 ttcaaggact tggtccaggt tgtagccggc tgtctgtcgc cagtccccaa cgaaatcttc
     3781 gagtgtgaag acgccgctcg agccgagaga aacgtggaca ggaaacgcac catatccccc
     3841 tgcctggatg ggtgtttttg gagaagcaca agcatataga ctaaaatcct ctgtttggaa
     3901 accagcccga ttcaaggagg gttctgatgc actgcggtga atttttggca atgagcgggc
     3961 cagcagctca atagaggcga gaatttgggg aaagagtggt ctctcatctc ttttcttttt
     4021 gaggcactct gccattaatc tcttcatggc ttttggacag ttactccgta ccttactgag
     4081 atctggagac aggtatcctc gtcccaccat aaaaattatc tggtccctgt tgttgatgtt
     4141 tgaataaggt aactgtccag tcatcaattc atacagaaca attccaaatg catatacatc
     4201 tgactgaaag ctgtatggat ttttatcttg cattctgatg acttctggtg ccatccacaa
     4261 aatggatcca gacaactgtt caaactgatg ggacccactc catcgagatt tcactgtagc
     4321 tagaccaaaa tcacctattt ttactgtgag gtcttcatga agaaatatat tattactctt
     4381 gaggtctctg tggatgattg acttggcgtg taagtaatcc atgccctgtg cagtctgtcg
     4441 tgcaatatct ataagtttga tcatctcaaa tttggtctca atgatatgga gatggtgata
     4501 caagctggag ccctcacacc actgggtaac aatagccagt tgtggctttg tggaatagcc
     4561 catgaagagt aggatattca catgtcgtgt tttcctgagt actcctactt catttttgaa
     4621 ggcttgtaac tgctgaggtg taggtgctgt cacattcaac attttcactg ccacatcacc
     4681 atgccacttt cccttgtaga ctgttccaaa tgatccagat ccaattcttt gtcccactgt
     4741 aatctgccca tcaggaatct cccaatcatc actcgagtcc cgtctaccaa gtgttttcat
     4801 tcgattcctg tcttctgagg atgaagatga cttcctttct cgctgaggtc ctggagattt
     4861 ctgtaaggct ttcacgttag ttagtgagcc aggtaatgag gcaggggggg tagcagacaa
     4921 acctgtggtt gatcctccat caccacgaaa tccttggtct ctaatcaagt catcaatatt
     4981 gacaggttct attgtgttta tatgcacatt gggagctgat gaggatcggt ctcgttgccc
     5041 aaattgattt cgatgatctt catctgctgg tcggaagggc tgtggaattg gaatggattt
     5101 tgaaggagac ggactggtga gaatttgggg cccaatagag tccgaggcgg gtgcggaagg
     5161 ggatgatcca gatgttaggg cagtctctgc taaggacgcc tcttcctgtg gtattgggtg
     5221 gtgttcaaag aacttggaga caaacagcaa atcaagttgg tcataattaa cacacatcag
     5281 tggaacttct gtactacaac gctggtgaaa tttataacca catgtttgac agcggaaacc
     5341 ctggaaaagc agctttcgac aaaagtcaca aaatgctaag gtgaaaaacg tttttcgtac
     5401 aaagttgtgt gttgtaagtg gaacattctc caacacttcc acatgcaatt cttctccagt
     5461 aagccaggaa atatcagtgt cccaaccaat tggtttcttc tctccatcct gaattctgta
     5521 aacagcacag cactctggga ttagacctct catcatcagt gctttcttta gactgtctcg
     5581 gactgtaact ccacaccttg caggtaccac tgtcctctgt ttgttgggca ggaagactct
     5641 aacgataggt ttttgtggtg acttggggtt gctccgtgcc acatctgtgg gattttgaaa
     5701 aactgaaaga gatgaaggta gcactgaaag gctagaagag gaagaagatg taacggtatc
     5761 cattgatgca gagctagaaa cagaaaaatc agttccgttc cccagagatt ccaataactg
     5821 ttgttctctt tgttggagtg catctagctt gctggtgtat tcttcatagg cctccagata
     5881 tattgatggt ggattatgct ccccaccaaa tttgtccaat agggcctcta tatgttcctg
     5941 tgtcaactta atcatttgtt tgatattcca cacctcctcc ggaatggcag ggtccgcagc
     6001 cgaagaggcc gcggcgccgg cgccggcgcc ggcctcgggc tccatgtccc cgttgaacag
     6061 agcctggccc ggctccgcgc cgccaccacc gccaccgctc agcgccgcca tggcgatcgc
     6121 tgcaaaaaga acaagtagct tgtattctat agtgtcacct aaatgggcga tctgacggtt
     6181 cactaaacca gctctgctga tataggcctc cctggcgcgc cactagttat taatagtaat
     6241 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     6301 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     6361 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     6421 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     6481 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     6541 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6601 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6661 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6721 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6781 taagcagagc tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc
     6841 acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca
     6901 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
     6961 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     7021 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
     7081 acagctctta aggctagagt attaatacga ctcactatag ggctagcgat cgccatggag
     7141 cacatacagg gagcttggaa gacgatcagc aatggttttg gattcaaaga tgccgtgttt
     7201 gatggctcca gctgcatctc tcctacaata gttcagcagt ttggctatca gcgccgggca
     7261 tcagatgatg gcaaactcac agatccttct aagacaagca acactatccg tgttttcttg
     7321 ccgaacaagc aaagaacagt ggtcaatgtg cgaaatggaa tgagcttgca tgactgcctt
     7381 atgaaagcac tcaaggtgag gggcctgcaa ccagagtgct gtgcagtgtt cagacttctc
     7441 cacgaacaca aaggtaaaaa agcacgctta gattggaata ctgatgctgc gtctttgatt
     7501 ggagaagaac ttcaagtaga tttcctggat catgttcccc tcacaacaca caactttgct
     7561 cggaagacgt tcctgaagct tgccttctgt gacatctgtc agaaattcct gctcaatgga
     7621 tttcgatgtc agacttgtgg ctacaaattt catgagcact gtagcaccaa agtacctact
     7681 atgtgtgtgg actggagtaa catcagacaa ctcttattgt ttccaaattc cactattggt
     7741 gatagtggag tcccagcact accttctttg actatgcgtc gtatgcgaga gtctgtttcc
     7801 aggatgcctg ttagttctca gcacagatat tctacacctc acgccttcac ctttaacacc
     7861 tccagtccct catctgaagg ttccctctcc cagaggcaga ggtcgacatc cacacctaat
     7921 gtccacatgg tcagcaccac cctgcctgtg gacagcagga tgattgagga tgcaattcga
     7981 agtcacagcg aatcagcctc accttcagcc ctgtccagta gccccaacaa tctgagccca
     8041 acaggctggt cacagccgaa aacccccgtg ccagcacaaa gagagcgggc accagtatct
     8101 gggacccagg agaaaaacaa aattaggcct cgtggacaga gagattcaag ctattattgg
     8161 gaaatagaag ccagtgaagt gatgctgtcc actcggattg ggtcaggctc ttttggaact
     8221 gtttataagg gtaaatggca cggagatgtt gcagtaaaga tcctaaaggt tgtcgaccca
     8281 accccagagc aattccaggc cttcaggaat gaggtggctg ttctgcgcaa aacacggcat
     8341 gtgaacattc tgcttttcat ggggtacatg acaaaggaca acctggcaat tgtgacccag
     8401 tggtgcgagg gcagcagcct ctacaaacac ctgcatgtcc aggagaccaa gtttcagatg
     8461 ttccagctaa ttgacattgc ccggcagacg gctcagggaa tggactattt gcatgcaaag
     8521 aacatcatcc atagagacat gaaatccaac aatatatttc tccatgaagg cttaacagtg
     8581 aaaattggag attttggttt ggcaacagta aagtcacgct ggagtggttc tcagcaggtt
     8641 gaacaaccta ctggctctgt cctctggatg gccccagagg tgatccgaat gcaggataac
     8701 aacccattca gtttccagtc ggatgtctac tcctatggca tcgtattgta tgaactgatg
     8761 acgggggagc ttccttattc tcacatcaac aaccgagatc agatcatctt catggtgggc
     8821 cgaggatatg cctccccaga tcttagtaag ctatataaga actgccccaa agcaatgaag
     8881 aggctggtag ctgactgtgt gaagaaagta aaggaagaga ggcctctttt tccccagatc
     8941 ctgtcttcca ttgagctgct ccaacactct ctaccgaaga tcaaccggag cgcttccgag
     9001 ccatccttgc atcgggcagc ccacactgag gatatcaatg cttgcacgct gaccacgtcc
     9061 ccgaggctgc ctgtcttcgt ttctctcgag ccaaccactg aggatctgta ctttcagagc
     9121 gataacgatg gatccgaaat cggtactggc tttccattcg acccccatta tgtggaagtc
     9181 ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc
     9241 ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg
     9301 acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa accagacctg
     9361 ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc cctgggtctg
     9421 gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca ctgggccaag
     9481 cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc tatcccgacc
     9541 tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac caccgacgtc
     9601 ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc
     9661 gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct gaatcctgtt
     9721 gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga gccagcgaac
     9781 atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt cccgaagctg
     9841 ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg cctggccaaa
     9901 agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct gcaagaagac
     9961 aacccggacc tgatcggcag cgagatcgcg cgctggctgt ctactctgga gatttccggt
    10021 taatagaatt ctagagtcga cctgca
//
