LOCUS       sequence-479888-       10024 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Express NanoLuc-TCF4(biased)-beta-Catenin-HaloTag Fusion Protein in 
            Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoLuc-TCF4(biased)-beta-Catenin-HaloTag
            Bidi...
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    61..182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(72..91)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(297..330)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        340..387
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             419..1213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(473..492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1083..1102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(1371..1959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1451..1470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2085..2133
                     /note="synthetic polyadenylation signal"
     CDS             complement(2157..2555)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      2552..2561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2586..2943)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2600..2735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2752..2943
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2900..2920
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    3022..3103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5184..5696)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     promoter        complement(5725..5743)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5726..5743)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        5863..6166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6167..6370
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6321..6341
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          6506..6638
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6621..6640
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     6682..6701
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6682..6700
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6714..9056
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             6714..9056
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             9078..9098
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             9114..9998
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
ORIGIN
        1 ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga
       61 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      121 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      181 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
      241 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagaat tctaatataa
      301 cttcgtatag catacattat acgaagttat ggatccactg ttgacaatta atcatcggca
      361 tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat
      421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1201 cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca
     1261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1921 tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct
     1981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2041 tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac
     2101 acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag
     2161 ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca
     2221 tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac
     2281 cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg
     2341 ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca
     2401 gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg
     2461 gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg
     2521 agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg
     2581 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2641 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2701 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2761 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2821 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2881 gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct
     2941 cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgcaa
     3001 gacgcgttaa ttaagacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     3181 ctacaaatgt ggtatggctg attatgatcc gcggccgcaa tacgtcgacg ttatcagctg
     3241 acttcgtacg agagcctagg tgtttctaga ggatccccgg gtaccgagcc cgaattcgtt
     3301 taaacttcta aagacttggt gacgagcgac agcggctggg gctgggtccc ggccagggag
     3361 ctgtgggaat gtaaggaaga agtcgacggc tgtgcaattg atgaggaggg ggcggcaggc
     3421 tgcaaagcgg ctgggggcag gtccagggcc ccgttgggac agagggcgga ggccttgtgg
     3481 gtggcctcag cgagcaggag ggcgggtggc ggaggcatca tggacaggtg ggccaggggg
     3541 tcgggcttca gggacagcga cagaggctgg gtctgctcag tctgtgactt ggcgtctcgg
     3601 ggaggggagc ctagcaggtt cggggagggg ggaggcgaat ctagtaagct tccatctgaa
     3661 gagggtgggc tgaggcagct gccttcacct tgtatgtagc gaacgcactt tttttttctc
     3721 ctgcaagggc cgcaccagtt attctgttga tcaaggccaa agcgcgctcg gcatttctta
     3781 ggagcgctca ggtctgtaat cggaggaagt gaaaggcaag gatttaggaa acattcgctg
     3841 tgttcattgg tctctcccgg ctgcttgtcc cttttcctct tcttcttctt tccatagtta
     3901 tcccgcgcgg accagccggg gtacagttgc atatgaagct gtcgctcctt ccgggccagc
     3961 tcgtagtatt tcgcttgctc ttctctggac agtgcatgcc acctccgccc aaggatctgg
     4021 ttgatggccg cgctttcttt caacgtgcac tcagctacga cctttgctct catttccttc
     4081 atatacaaca tgaatgcatt aagaggtttc tttatgtggg gcttcttctt ttcttcttcc
     4141 tttttggagt cctgatgctt tgaactatgg agtgagccga catcactctg ggacgattcc
     4201 tgtttgactg ttggtgtgac tatggccgga tgcggaatgc ccgtcgtgtg tagcgtatga
     4261 tgtggtggga ccatatgggg agggaacctg gacatggaag cattgacggt cagagctgtg
     4321 gggtaggggt gtctgaatcc tcctgtcgtg attgggtaca ctggttgacc ttgctgtggt
     4381 actaaccatc ctagcggatg ggggatttgt cctacggtgc caggcgatag tgggtaatac
     4441 ggggatatat ctggagggtg cggaggccgt gggattcctg ttttggggtc tacgtcggct
     4501 ggtaagtgtg gaggtgggtt tcccggcgtg aagtgttcat tgctgtacgt gataagaggc
     4561 gtgagggggt ggacatggtg agggtgctgc accactggca ctttgttaga gacaatgtgt
     4621 gccggtgatg gggaccgggc atccttgagg gcttgtctac tctggaggct ccctgcctgg
     4681 acatcaagca gtggccattt catctggaga tactgaactg caatctggtg ttcaatcgtt
     4741 ttgtacgcag agtaatgtgt gctgccggac tgaaaatgga gggttcgggc ggtgggcgag
     4801 agcgatccgt tggggaggta ggggctcgtc aggtcgggga tcatgatgaa ggggtagccg
     4861 ggatacggtg gccccttaaa gagccctcca tcttgcctct tggccgcttc ttccaaactt
     4921 tcccgggatt tgtctcggaa actttcggag cgaggcggag gccgtctttc cgcctcggaa
     4981 tcggaggagc tgttttgatt cgtttctgat tcattgacta gagacgattt gacatcagct
     5041 aaatccctct ctgccgagga gttttcggag ctcttctcct cctgttcgcc ctcgtctttg
     5101 aaggaaatca gttcgtcgtt ggcgcctagg tcatcccctc caccgccgtt cagctgcggc
     5161 atggcgatcg cgccgctcga gcccgccaga atgcgttcgc acagccgcca gccggtcact
     5221 ccgttgatgg ttactcggaa cagcagggag ccgtcggggt tgatcaggcg ctcgtcgata
     5281 attttgttgc cgttccacag ggtccctgtt acagtgatct ttttgccgtc gaacacggcg
     5341 atgccttcat acggccgtcc gaaatagtcg atcatgttcg gcgtaacccc gtcgattacc
     5401 agtgtgccat agtgcaggat caccttaaag tgatgatcat ccacagggta caccacctta
     5461 aaaatttttt cgatctggcc catttggtcg ccgctcagac cttcatacgg gatgatgaca
     5521 tggatgtcga tcttcagccc attttcaccg ctcaggacaa tcctttggat cggagttacg
     5581 gacaccccga gattctgaaa caaactggac acacctccct gttcaaggac ttggtccagg
     5641 ttgtagccgg ctgtctgtcg ccagtcccca acgaaatctt cgagtgtgaa gaccatggtt
     5701 gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatgggcgat ctgacggttc
     5761 actaaaccag ctctgctgat ataggcctcc ctggcgcgcc actagttatt aatagtaatc
     5821 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5881 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5941 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6001 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     6061 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     6121 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6181 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6241 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6301 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6361 aagcagagct ggtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
     6421 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
     6481 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     6541 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     6601 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     6661 cagctcttaa ggctagagta ttaatacgac tcactatagg gctagcgatc gccatggcta
     6721 ctcaagctga tttgatggag ttggacatgg ccatggaacc agacagaaaa gcggctgtta
     6781 gtcactggca gcaacagtct tacctggact ctggaatcca ttctggtgcc actaccacag
     6841 ctccttctct gagtggtaaa ggcaatcctg aggaagagga tgtggatacc tcccaagtcc
     6901 tgtatgagtg ggaacaggga ttttctcagt ccttcactca agaacaagta gctgatattg
     6961 atggacagta tgcaatgact cgagctcaga gggtacgagc tgctatgttc cctgagacat
     7021 tagatgaggg catgcagatc ccatctacac agtttgatgc tgctcatccc actaatgtcc
     7081 agcgtttggc tgaaccatca cagatgctga aacatgcagt tgtaaacttg attaactatc
     7141 aagatgatgc agaacttgcc acacgtgcaa tccctgaact gacaaaactg ctaaatgacg
     7201 aggaccaggt ggtggttaat aaggctgcag ttatggtcca tcagctttct aaaaaggaag
     7261 cttccagaca cgctatcatg cgttctcctc agatggtgtc tgctattgta cgtaccatgc
     7321 agaatacaaa tgatgtagaa acagctcgtt gtaccgctgg gaccttgcat aacctttccc
     7381 atcatcgtga gggcttactg gccatcttta agtctggagg cattcctgcc ctggtgaaaa
     7441 tgcttggttc accagtggat tctgtgttgt tttatgccat tacaactctc cacaaccttt
     7501 tattacatca agaaggagct aaaatggcag tgcgtttagc tggtgggctg cagaaaatgg
     7561 ttgccttgct caacaaaaca aatgttaaat tcttggctat tacgacagac tgccttcaaa
     7621 ttttagctta tggcaaccaa gaaagcaagc tcatcatact ggctagtggt ggaccccaag
     7681 ctttagtaaa tataatgagg acctatactt acgaaaaact actgtggacc acaagcagag
     7741 tgctgaaggt gctatctgtc tgctctagta ataagccggc tattgtagaa gctggtggaa
     7801 tgcaagcttt aggacttcac ctgacagatc caagtcaacg tcttgttcag aactgtcttt
     7861 ggactctcag gaatctttca gatgctgcaa ctaaacagga agggatggaa ggtctccttg
     7921 ggactcttgt tcagcttctg ggttcagatg atataaatgt ggtcacctgt gcagctggaa
     7981 ttctttctaa cctcacttgc aataattata agaacaagat gatggtctgc caagtgggtg
     8041 gtatagaggc tcttgtgcgt actgtccttc gggctggtga cagggaagac atcactgagc
     8101 ctgccatctg tgctcttcgt catctgacca gccgacacca agaagcagag atggcccaga
     8161 atgcagttcg ccttcactat ggactaccag ttgtggttaa gctcttacac ccaccatccc
     8221 actggcctct gataaaggct actgttggat tgattcgaaa tcttgccctt tgtcccgcaa
     8281 atcatgcacc tttgcgtgag cagggtgcca ttccacgact agttcagttg cttgttcgtg
     8341 cacatcagga tacccagcgc cgtacgtcca tgggtgggac acagcagcaa tttgtggagg
     8401 gggtccgcat ggaagaaata gttgaaggtt gtaccggagc ccttcacatc ctagctcggg
     8461 atgttcacaa ccgaattgtt atcagaggac taaataccat tccattgttt gtgcagctgc
     8521 tttattctcc cattgaaaac atccaaagag tagctgcagg ggtcctctgt gaacttgctc
     8581 aggacaagga agctgcagaa gctattgaag ctgagggagc cacagctcct ctgacagagt
     8641 tacttcactc taggaatgaa ggtgtggcga catatgcagc tgctgttttg ttccgaatgt
     8701 ctgaggacaa gccacaagat tacaagaaac ggctttcagt tgagctgacc agctctctct
     8761 tcagaacaga gccaatggct tggaatgaga ctgctgatct tggacttgat attggtgccc
     8821 agggagaacc ccttggatat cgccaggatg atcctagcta tcgttctttt cactctggtg
     8881 gatatggcca ggatgccttg ggtatggacc ccatgatgga acatgagatg ggtggccacc
     8941 accctggtgc tgactatcca gttgatgggc tgccagatct ggggcatgcc caggacctca
     9001 tggatgggct gcctccaggt gacagcaatc agctggcctg gtttgatact gacctggttt
     9061 ctctcgagcc aaccactgag gatctgtact ttcagagcga taacgatgga tccgaaatcg
     9121 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
     9181 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
     9241 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
     9301 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
     9361 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
     9421 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
     9481 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
     9541 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
     9601 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
     9661 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
     9721 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat
     9781 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
     9841 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
     9901 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     9961 agatcgcgcg ctggctgtct actctggaga tttccggtta atagaattct agagtcgacc
    10021 tgca
//
