LOCUS       sequence-480223-       10384 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Lentiviral vector to generate MFSD6 stable expressing cell line 
            under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Varanese L, Xu L, Peters CE, Pintilie G, Roberts DS, Raj S, Liu M, 
            Ooi YS, Diep J, Qiao W, Richards CM, Callaway J, Bertozzi CR, Jabs 
            S, de Vries E, van Kuppeveld FJM, Nagamine CM, Chiu W, Carette JE
  TITLE     MFSD6 is an entry receptor for enterovirus D68.
  JOURNAL   Nature. 2025 Mar 25. doi: 10.1038/s41586-025-08908-0.
  PUBMED    40132641
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Mar 25. doi: 10.1038/s41586-025-08908-0."
COMMENT     Sequence Label: pLenti-CMV-MFSD6-Puro
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2998..3301
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3302..3505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3456..3476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3502..3526
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5997..6026
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6045..6068
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6101..6689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6154..6174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6572..6583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6703..7202
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6738..6761)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7135..7154
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7223..7822
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7223..7242)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7719..7739
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7960..8193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8265..8386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8302..8321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8356..8375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8426..8561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8581..8600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8582..8600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8610..8627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8610..8626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8619..8641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8768..9223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8855..8874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9065..9086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9249..9353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9354..9422,9423..10214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9572..9591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     3001 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3061 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3121 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3181 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3241 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3301 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3361 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3421 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3481 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     3541 tccacgctgt tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg
     3601 cagatatggc agatgataaa gttgctatct taacggatga tgaagaggaa cagaagagaa
     3661 agtatgtgct tgcagatccc tttaatggta tttccaggga accagaacca ccttcgaatg
     3721 aaacaccttc ctccacagaa acatctgcta ttcctgagga ggaaatagac tggatagaga
     3781 aacattgtgt taagataaac aacgatcttc taatttccaa ggtcttttat tttttctttt
     3841 actctgccta tggctctctc tatccccttt tgcctgtgta ttacaaacag ctgggaatgt
     3901 ctccaagcca gagtggacta ctagtaggta ttcgttactt cattgaattc tgcagtgccc
     3961 ccttttgggg tgtagttgca gaccgcttta aaaaaggcaa aattgtcctc ctcttttctc
     4021 ttttgtgttg ggttttattc aacctgggca ttggatttgt caaacctgct accttgagat
     4081 gtgtaccaaa gattcgccca acaactcacc ccaccaatgc aagtcaccag ttaactatcc
     4141 tgccaacaaa ttcttccttt acctctttcc tcaccatatc accaaaaatg cgtgagaaaa
     4201 gaaacctttt ggaaacaagg ctcaatgtct cagacaccgt tactttgcca acagctccaa
     4261 acatgaacag tgaacccact ctgcagcccc agacaggtga aattactaac cgtatgatgg
     4321 acttgacttt gaactcaagc acagcaaccc ctgtctcccc aggaagcgta accaaggaga
     4381 caaccactgt tattgttacc accaccaaat ctttaccttc tgaccaagtc atgcttgttt
     4441 atgatcaaca agaagttgaa gctatattct tggtgatctt ggtagttgtc ataataggag
     4501 aatttttcag tgcctcttct gtcacaatcg tagacacggt cacactccag tatctgggaa
     4561 aacacagaga tcgctatggg ttgcagcgca tgtggggctc cctgggctgg ggcctggcga
     4621 tgctgtctgt gggcatcggg atcgactaca cccacatcga agtgctcatc gatggaaagg
     4681 ggtgtaagcc ccccgagtac aggaattacc agatcgtctt catcgtcttc ggcgttctca
     4741 tgaccatggc cttgatcgtt gccactcagt tccggttccg ctacaaccat ttcaaaaacg
     4801 atgattctaa agggaaagag gtggagatcc cgcaggtgga aaggaacaac tctacagagt
     4861 cctctgagga gacaccaacc accacaagcc actcgcaggc cttcaacttt tgggacttaa
     4921 tcaagctgct ctgcagcgtg cagtatggct cagtgctgtt tgtggcttgg ttcatgggtt
     4981 ttggatatgg cttcgtgttc acctttctct actggcattt ggaagacctc aatggaacta
     5041 caaccctctt tggggtctgt tcagtcctga gtcatgtgtc tgagctgaca gcatattttt
     5101 ttagtcacaa gcttattgaa ttgatcggcc acatcagggt tctgtacatt ggcctggcct
     5161 gcaatacggc tcgctatatt tatatttcct acctggagaa tgcctggact gttctcccca
     5221 tggaagttct tcaaggagtg acacacgcgg ccatctgggc agcatgcatt tcttacctca
     5281 gtgcagccgt tccccctgag ctgaggacat ctgctcaggg catcctgcag ggccttcacc
     5341 tgggtttggg aagaggatgt ggtgccatga tcggaggcgt gttagtcaat tattttgggg
     5401 ctgctgcaac cttccgagga attggcatgg cctgcttggt gatcctactg ctctttgccc
     5461 tgatccagtg gctggcagtg ccagatgagg aagaagacaa gacaatgttg gcagaaagaa
     5521 ttcctgttcc ctccagtccc gttcctatag caaccatcga cttggtacag caacagacag
     5581 aagatgtcat gccacgcatt gagcccagac ttccacccaa gaaaactaag caccaggaag
     5641 aacaggaaga tgtgaacaaa ccagcctggg gagtcagctc ttctccctgg gtgacctttg
     5701 tctatgcact ctaccaaatt aaagagatga tgcaactcac aagagacaac cgtgcttctg
     5761 agatacagcc tttacagggg accaatgaga atagggaaaa ttctcctgct ggtagagccc
     5821 agcctgtccc atgtgagact cactctgacc catctagaaa ccagccatcc cctgacgcag
     5881 cagcatctca gacgcagacc agccccgctc accccagtgt ggacccgtgc acagaggaga
     5941 gtgaagagca gcaggctcag ctggccgcgg gaggacacac gcgtacgcgg ccgctcgagc
     6001 agaaactcat ctcagaagag gatctggcag caaatgatat cctggattac aaggatgacg
     6061 acgataaggt ttaaatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
     6121 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6181 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6241 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6301 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6361 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6421 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6481 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6541 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6601 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6661 tcggatctcc ctttgggccg cctccccgcc tggaattcta ccgggtaggg gaggcgcttt
     6721 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     6781 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     6841 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagtcc ccccccgccc
     6901 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     6961 agatggacag caccgctgag caatggaggc gggtaggcct ttggggcagc ggcaaatagc
     7021 agctttgctc cttcgctttc tgggctcagg ggcggggagg gggggggccg gggggcgggc
     7081 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     7141 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     7201 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7261 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7321 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7381 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7441 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7501 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7561 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7621 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7681 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     7741 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     7801 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     7861 cgcacgaccc catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag
     7921 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc
     7981 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8041 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8101 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8161 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     8221 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8401 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8461 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8521 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8581 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8641 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8701 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8761 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8821 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8881 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8941 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9001 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9061 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9121 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9181 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9961 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10021 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10081 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10141 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10201 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10261 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10381 cttc
//
