LOCUS       sequence-480224-        7225 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  5' AAVLINK plasmid for PRR12 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Zhonghua Lu Lab
  TITLE     Zhonghua Lu AAV Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' PRR12
FEATURES             Location/Qualifiers
     source          1..7225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   4499..4628
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      4703..5158
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4790..4809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5000..5021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5175..5194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5294..5316
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5354..5372)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5440..5544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5545..5613,5614..6405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5763..5782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6576..7164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7065..7084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctgaca ggaactaccc cagcgccggc ttcggggacc cgctcggcgc
      781 cggggcggga tggagttacg agaggtcagc gaaagctagc ttggtttatg gcagctccag
      841 gacctcgcac cccgagacgg acatcttaca ccgccaggcc tatgcggccc cccacccact
      901 gcaaagctat gccaccaacc accacccggc aggcctctct ggactcttcg acactggcct
      961 ccaccacgcg ggctcagcag ggcccgacgc ctccgtcatg aacctcatct cggccctgga
     1021 atcccggggc ccccagcctg gcccctccgc ctcctctctc ctctcccagt tccgcagtcc
     1081 ttcctggcaa acagccatgc acacgccagg ccccacggag ctcttcatct cgggtgccct
     1141 gccgggttcc agcacctttc cgtcctcatc tgccctgtcg gcttaccaac acccggcttc
     1201 cttcggcagc cgccccttcc cagtgccctc gtccctcagc ctccaggacc ccccattcag
     1261 ccctccagct aacgggctcc tgtcccctca tgacgtgctg cacctgaagc cctcgcaggc
     1321 acccacggtg ccctcttcac tgggcttcga gcgcctggca gggggcggtg tcttggggcc
     1381 agctggtctc ggtccagccc agaccccccc ttaccgccct ggccccccag acccaccacc
     1441 acctcctcgc cacctcccaa ctcagttcaa cctgctggct tcctcttccg ctgccgccgc
     1501 cgctgccgag cagtcctccc cacagctcta taacttctcg ggtgctgccc cgggcccacc
     1561 gccacctgag cgggccctgc cacgccagga cacggtcatc aagcactacc agcggccagc
     1621 cagtgcccag cccccaccac ccccgccacc agcccatgcg ctccagcact atctgagctg
     1681 tggaggcagc tacccctcca tgggccaccg ggccaacctg gcctgcagcc ccctgggtgg
     1741 tggggagccc tccccgggtg ctggggagcc tagcaaggct ggtcccagcg gagccacggc
     1801 tggggcatct ggccgggcca cgggccctga ggcagcaggg ggcggtgggg ctgggggtgg
     1861 tggtggaggt taccgcccca tcattcagtc gcctgggtac aagacgggca aaggtggtta
     1921 tggagcagct gccgggggtg ccaccaggcc ccccccaccc cgttcgaccg ccacccccaa
     1981 atgtcagagc ctgggtgggc cagcagccgc ctatgccact gggaaggcct ctggggctgg
     2041 aggggcaggg ggccaggctt attcccccgg tcagcctcaa gggcttctgg gaccccaggc
     2101 ctacgggcaa gggtttggag gggggcaggc acaggacttg agcaaagccc ccagctactc
     2161 agggggcccc ccacagcccc ccagcggccc ccctcctcct ggcctggcca catgtcagag
     2221 ctactccccg gaccagctgc aggggcagct gtatggggtg cagggcgagc catacccagg
     2281 gccagccgcc cactcccagg ggctgcccac agccagcccc tcgctcagct acagtaccgg
     2341 ccattcccca gcgctctcgg gccatggggg tggctgggga cccagctccc tgggaggcgg
     2401 cggtgaggcc agcccatctc acatcattcg tccgctccag tcaccgcctg ccaccggccg
     2461 tccacctgga gtcggctctc caggagcccc tggcaaatac ctgagctcag tcttggcctc
     2521 agcgcctttc ctggcacctc cgggagctgg cagctatgca gctggagcag gtggctacaa
     2581 gggcaagggg gatggctcgg agctgctggc gggcccaggt gggcctcctg cggagcgcac
     2641 agaggatgag gagttcctta tccagcacct cttgcaggcg cccagccctc ctcggacctc
     2701 aggggcggac ggcttggtgg gcgaggacgg ggcagcagat gcctctaagg gacttggggg
     2761 gagtggcggg gccgggggac caccgggtac accctacgag ttggccaagg aagaccccca
     2821 gaggtaccac ctgcagagtg tcatccgcac cagtgccagc ctggatgagg gtgccactgc
     2881 ggcactggag ctgggcctgg ggaggctgaa ggagaagaag aaagggccag agcggggtgg
     2941 cgagaccccc gaggggctgg ccacctctgt tgtccactac ggggcaggcg ccaaggagct
     3001 gggggccttc ttgcaaaaga gccctccgcc cccacctccc acggcccagt ctacccagcc
     3061 cactccccat ggcctccttc tggaggccgg gggccctgac ctcccactgg tgctgcctcc
     3121 gcctcccccc cagctgctcc cctcggtcct cagccatgcc cccagtccct ctcccagcgc
     3181 ctccaaagtc ggcgtccacc tccttgagcc agccacccgc gatggggcac cccagccacc
     3241 tccaccgcca cccccgcctc caccacccat gcccctgcag ctcgaggccc acctccgcag
     3301 ccatggcctg gagcccgcgg cccccagccc ccgcctgcga cccgaggaga gcctggatcc
     3361 gccaggcgcc atgcaggaat tgctcggggc tctggagccg ctgcccccgg cgcctgggga
     3421 tactggcgta ggcccgccaa actcggaggg caaggatccc gcaggcgcct accgcagccc
     3481 cagcccgcaa ggcaccaagg cgccgcgttt cgtgccgctc acctccatct gcttccctga
     3541 ctccttgctc caagacgagg agcgcagctt cttccccacc atggaggaga tgttcggtgg
     3601 aggggccgcg gacgactacg gcaaggccgg gccacctgag gacgaggggg accccaaggc
     3661 tggcgctggg ccaccccccg gcccccctgc ttatgatccc tatgggccct actgtcctgg
     3721 ccgggcgtcg ggagccgggc ccgagacacc gggcctgggc ctggacccca acaaaccgcc
     3781 tgaactgccc tccacggtca acgccgagcc gctgggcctg atccagagtg gcccccacca
     3841 ggcggcgcca ccacccccgc ctccgccacc gccgcctccc gcgccggcct ccgaacccaa
     3901 gggtggcctc acctcgccca tcttctgctc taccaagcca aagaagctgc tcaagacatc
     3961 ctccttccac ctgctgcggc gccgcgaccc acccttccag acccccaaga agctgtacgc
     4021 ccaggagtac gagttcgagg cggacgagga caaggccgat gttcccgccg acatccgcct
     4081 caacccccgg cgcttgcctg acctggtctc cagctgccgc tcccgtccgg ccctctcgcc
     4141 actgggggac atcgacttct gcccacccaa cccaggaccc gatggccccc ggcgccgtgg
     4201 ccgcaagccc acgaaggcga aacgtgatgg gccaccccgg ccacggggga ggccccggat
     4261 ccgccccctg gaggtcccga ccactgcggg gcccgcctcg gcctccacgc ccaccgatgg
     4321 cgccaagaaa ccccggggcc ggggccgagg ccggggtcga aaggctgagg aggcaggtaa
     4381 gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt ataggatact
     4441 ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag
     4501 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4561 gggcgaccaa aggtcgcccg acgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     4621 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4681 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4741 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     4801 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4861 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     4921 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4981 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     5041 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     5101 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     5161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5221 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5281 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5341 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5401 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5761 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6061 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6721 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7201 ttttgctggc cttttgctca catgt
//
