LOCUS       sequence-480225-       11008 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Lentiviral vector to generate mNeon-tagged MFSD6 stable expressing 
            cell line under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Varanese L, Xu L, Peters CE, Pintilie G, Roberts DS, Raj S, Liu M, 
            Ooi YS, Diep J, Qiao W, Richards CM, Callaway J, Bertozzi CR, Jabs 
            S, de Vries E, van Kuppeveld FJM, Nagamine CM, Chiu W, Carette JE
  TITLE     MFSD6 is an entry receptor for enterovirus D68.
  JOURNAL   Nature. 2025 Mar 25. doi: 10.1038/s41586-025-08908-0.
  PUBMED    40132641
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Mar 25. doi: 10.1038/s41586-025-08908-0."
COMMENT     Sequence Label: pLenti-CMV-MFSD6-mNeon-Puro
FEATURES             Location/Qualifiers
     source          1..11008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2998..3301
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3302..3505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3456..3476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3502..3526
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5991..6698
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      6243..6252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6725..7313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6778..6798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7196..7207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7327..7826
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7362..7385)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             7847..8446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7847..7866)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8343..8363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8584..8817
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8889..9010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8926..8945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8980..8999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9050..9185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9205..9224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9206..9224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9234..9251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9234..9250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9243..9265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9392..9847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9479..9498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9689..9710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9873..9977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9978..10046,10047..10838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10196..10215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     3001 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3061 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3121 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3181 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3241 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3301 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3361 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3421 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3481 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     3541 tccacgctgt tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg
     3601 cagatatggc agatgataaa gttgctatct taacggatga tgaagaggaa cagaagagaa
     3661 agtatgtgct tgcagatccc tttaatggta tttccaggga accagaacca ccttcgaatg
     3721 aaacaccttc ctccacagaa acatctgcta ttcctgagga ggaaatagac tggatagaga
     3781 aacattgtgt taagataaac aacgatcttc taatttccaa ggtcttttat tttttctttt
     3841 actctgccta tggctctctc tatccccttt tgcctgtgta ttacaaacag ctgggaatgt
     3901 ctccaagcca gagtggacta ctagtaggta ttcgttactt cattgaattc tgcagtgccc
     3961 ccttttgggg tgtagttgca gaccgcttta aaaaaggcaa aattgtcctc ctcttttctc
     4021 ttttgtgttg ggttttattc aacctgggca ttggatttgt caaacctgct accttgagat
     4081 gtgtaccaaa gattcgccca acaactcacc ccaccaatgc aagtcaccag ttaactatcc
     4141 tgccaacaaa ttcttccttt acctctttcc tcaccatatc accaaaaatg cgtgagaaaa
     4201 gaaacctttt ggaaacaagg ctcaatgtct cagacaccgt tactttgcca acagctccaa
     4261 acatgaacag tgaacccact ctgcagcccc agacaggtga aattactaac cgtatgatgg
     4321 acttgacttt gaactcaagc acagcaaccc ctgtctcccc aggaagcgta accaaggaga
     4381 caaccactgt tattgttacc accaccaaat ctttaccttc tgaccaagtc atgcttgttt
     4441 atgatcaaca agaagttgaa gctatattct tggtgatctt ggtagttgtc ataataggag
     4501 aatttttcag tgcctcttct gtcacaatcg tagacacggt cacactccag tatctgggaa
     4561 aacacagaga tcgctatggg ttgcagcgca tgtggggctc cctgggctgg ggcctggcga
     4621 tgctgtctgt gggcatcggg atcgactaca cccacatcga agtgctcatc gatggaaagg
     4681 ggtgtaagcc ccccgagtac aggaattacc agatcgtctt catcgtcttc ggcgttctca
     4741 tgaccatggc cttgatcgtt gccactcagt tccggttccg ctacaaccat ttcaaaaacg
     4801 atgattctaa agggaaagag gtggagatcc cgcaggtgga aaggaacaac tctacagagt
     4861 cctctgagga gacaccaacc accacaagcc actcgcaggc cttcaacttt tgggacttaa
     4921 tcaagctgct ctgcagcgtg cagtatggct cagtgctgtt tgtggcttgg ttcatgggtt
     4981 ttggatatgg cttcgtgttc acctttctct actggcattt ggaagacctc aatggaacta
     5041 caaccctctt tggggtctgt tcagtcctga gtcatgtgtc tgagctgaca gcatattttt
     5101 ttagtcacaa gcttattgaa ttgatcggcc acatcagggt tctgtacatt ggcctggcct
     5161 gcaatacggc tcgctatatt tatatttcct acctggagaa tgcctggact gttctcccca
     5221 tggaagttct tcaaggagtg acacacgcgg ccatctgggc agcatgcatt tcttacctca
     5281 gtgcagccgt tccccctgag ctgaggacat ctgctcaggg catcctgcag ggccttcacc
     5341 tgggtttggg aagaggatgt ggtgccatga tcggaggcgt gttagtcaat tattttgggg
     5401 ctgctgcaac cttccgagga attggcatgg cctgcttggt gatcctactg ctctttgccc
     5461 tgatccagtg gctggcagtg ccagatgagg aagaagacaa gacaatgttg gcagaaagaa
     5521 ttcctgttcc ctccagtccc gttcctatag caaccatcga cttggtacag caacagacag
     5581 aagatgtcat gccacgcatt gagcccagac ttccacccaa gaaaactaag caccaggaag
     5641 aacaggaaga tgtgaacaaa ccagcctggg gagtcagctc ttctccctgg gtgacctttg
     5701 tctatgcact ctaccaaatt aaagagatga tgcaactcac aagagacaac cgtgcttctg
     5761 agatacagcc tttacagggg accaatgaga atagggaaaa ttctcctgct ggtagagccc
     5821 agcctgtccc atgtgagact cactctgacc catctagaaa ccagccatcc cctgacgcag
     5881 cagcatctca gacgcagacc agccccgctc accccagtgt ggacccgtgc acagaggaga
     5941 gtgaagagca gcaggctcag ctggccgcgg gaggacacgg atccggcgga gtgagcaagg
     6001 gcgaggagga taacatggcc tctctcccag cgacacatga gttacacatc tttggctcca
     6061 tcaacggtgt ggactttgac atggtgggtc agggcaccgg caatccaaat gatggttatg
     6121 aggagttaaa cctgaagtcc accaagggtg acctccagtt ctccccctgg attctggtcc
     6181 ctcatatcgg gtatggcttc catcagtacc tgccctaccc tgacgggatg tcgcctttcc
     6241 aggccgccat ggtagatggc tccggctacc aagtccatcg cacaatgcag tttgaagatg
     6301 gtgcctccct tactgttaac taccgctaca cctacgaggg aagccacatc aaaggagagg
     6361 cccaggtgaa ggggactggt ttccctgctg acggtcctgt gatgaccaac tcgctgaccg
     6421 ctgcggactg gtgcaggtcg aagaagactt accccaacga caaaaccatc atcagtacct
     6481 ttaagtggag ttacaccact ggaaatggca agcgctaccg gagcactgcg cggaccacct
     6541 acacctttgc caagccaatg gcggctaact atctgaagaa ccagccgatg tacgtgttcc
     6601 gtaagacgga gctcaagcac tccaagaccg agctcaactt caaggagtgg caaaaggcct
     6661 ttaccgatgt gatgggcatg gacgagctgt acaagtagat ccagcacagt ggcggccgct
     6721 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6781 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6841 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6901 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6961 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7021 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7081 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7141 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7201 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7261 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     7321 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     7381 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     7441 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     7501 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     7561 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     7621 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     7681 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     7741 gggcctccgg aggccccgca ttctgcacgc ctcaaaagcg cacgtctgcc gcgctgttct
     7801 cctcttcctc atctcccggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     7861 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     7921 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7981 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     8041 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     8101 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     8161 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     8221 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8281 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8341 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8401 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     8461 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     8521 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8581 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8641 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8701 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8761 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8821 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8881 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9001 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9061 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9121 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9181 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9241 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9301 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9361 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9541 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9601 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9661 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9721 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9781 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9841 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9901 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9961 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10021 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10081 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10141 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10201 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10261 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10321 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10381 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10441 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10501 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10561 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10621 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10681 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10741 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10801 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10981 accccgtaga aaagatcaaa ggatcttc
//
