LOCUS       sequence-480229-       13059 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F
  TITLE     Point mutations in TOR confer Rheb-independent growth in fission 
            yeast and nutrient-independent mammalian TOR signaling in mammalian 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3514-9.
  PUBMED    17360675
REFERENCE   2  (bases 1 to 13059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 27. 104(9):3514-9."
COMMENT     Sequence Label: pcDNA3-AU1-mTOR-E2419K
FEATURES             Location/Qualifiers
     source          1..13059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     867..886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        867..885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      904..913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3779..3788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7000..7272
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     promoter        complement(8612..8630)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8613..8630)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8650..8667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8656..8880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8926..9354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9013..9032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9223..9244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9363..9383)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9368..9697
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9548..9683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9610..9629
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9764..10558
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9818..9837)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10428..10447
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10732..10853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10769..10788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10823..10842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10902..10918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10902..10918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10915..10937)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10926..10942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10950..10956,10957..10974,10975..10980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10995..11016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11133..11150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11304..11892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11384..11403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12063..12854,12855..12923))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12686..12705
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12924..13028)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      841 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttct
      901 cgagccacca tggacacgta caggtacatc cttgggacag gccctgccac ggccaccgcc
      961 ggtgccgcca catccagcaa cgtgagcgtt ctgcagcagt tcgccagtgg gctgaagagc
     1021 cggaatgagg agaccagggc caaagcagcc aaggagctcc agcactatgt caccatggaa
     1081 cttcgagaga tgagtcagga ggagtctact cgcttctatg accagctgaa ccatcacatt
     1141 tttgaactgg tttccagctc agacgccaat gagaggaagg gtggcatctt ggccattgcc
     1201 agcctcattg gagtggaagg tgggaattcc accaggattg gcagatttgc caactacctt
     1261 cggaacctcc tcccctcaag tgatccagtt gtcatggaga tggcatccaa ggccattggc
     1321 cgccttgcaa tggcagggga cactttcact gctgagtatg tggagtttga agtgaagcga
     1381 gccttggagt ggctgggtgc tgaccgaaat gagggccgga gacatgcagc tgtcctcgtt
     1441 ctccgtgagc tggccatcag cgtccccacc ttcttcttcc agcaagttca gcccttcttt
     1501 gacaacattt ttgtggctgt gtgggacccc aagcaggcca tccgtgaagg agctgtggct
     1561 gcccttcgtg cctgtctgat tcttaccacg cagcgggagc cgaaggagat gcagaagcct
     1621 cagtggtaca ggcacacatt tgaagaagca gagaaaggtt ttgatgagac cctggccaaa
     1681 gagaagggta tgaaccgaga tgatcgaatc cacggggcct tgctgatcct caacgagctc
     1741 gtccgaatca gcagcatgga gggagagcgt ctgagagagg agatggagga aatcacccag
     1801 cagcagctgg tacatgacaa gtactgcaaa gacctaatgg gctttgggac aaagcctcgg
     1861 cacatcactc ccttcaccag cttccaggct gtgcagcccc agcagtcaaa cgccttggtg
     1921 ggactgctgg ggtacagctc ccaccaaggc ctaatggggt ttggggcttc ccccagccct
     1981 acaaagtcca ctctggtgga aagccgttgt tgcagagact tgatggaaga gaaatttgat
     2041 caggtgtgcc agtgggtgct gaaatgtagg agcagcaaga actcactgat ccaaatgaca
     2101 atccttaatc tgttgccccg cttggctgca ttccgaccgt ctgccttcac agatacccag
     2161 tacctgcaag acaccatgaa ccatgtcctg agctgtgtca agaaggaaaa ggaacggacc
     2221 gcagcgttcc aggccctagg gctgctttct gtggcggtga ggtccgagtt taaggtctac
     2281 ctgccccgag tacttgacat catccgagca gccctgcctc caaaggactt tgcccacaag
     2341 aggcagaaaa ctgtgcaggt ggatgccaca gtgttcacgt gcatcagcat gctggcgcgg
     2401 gccatggggc caggcatcca gcaggacatc aaggagctgc tggagcccat gttggcagtg
     2461 ggcctgagcc ctgcgctcac tgctgtgctc tatgacctga gccggcagat tccgcagctg
     2521 aagaaagata ttcaggacgg gcttctgaag atgctgtccc tggtccttat gcacaaaccc
     2581 ctgcggcacc cgggcatgcc caaaggcctg gcccaccagc tggcctcccc aggtcttacc
     2641 accctccctg aggccagcga cgtggccagc atcactcttg cccttcgaac tctcggcagc
     2701 tttgaatttg aaggccactc tctgacccag ttcgtccgac actgcgcaga tcatttcctg
     2761 aacagtgagc acaaggagat ccgcatggaa gcagcccgca cctgctcccg cctgctcaca
     2821 ccctccatcc acctcatcag cggccatgcc catgtggtta gccagaccgc agtgcaagtg
     2881 gtagcagatg tgctcagcaa gctgcttgtg gtcggcataa cagatcctga tcctgatatc
     2941 cgctactgtg tcttggcctc cctggatgag cgcttcgatg cccacttggc ccaggcagaa
     3001 aacttacaag ctctgtttgt ggctctgaat gaccaggtct ttgagatccg agagctggcc
     3061 atctgcactg tgggccgact cagtagcatg aacccagcct ttgtcatgcc tttcctgcgc
     3121 aagatgctca tccagatttt gacagagctg gagcacagtg gcattgggag aatcaaggag
     3181 cagagtgccc gcatgctggg gcacctggtc tccaatgccc cccgcctcat ccgcccctat
     3241 atggagccta ttctgaaggc tttaattttg aaactcaaag atccagaccc tgacccaaac
     3301 ccgggcgtga tcaataacgt gttggccact ataggagaac tggctcaggt tagcggcctg
     3361 gagatgagga agtgggtgga cgagctcttt gtcatcatca tggacatgct gcaggactcc
     3421 tctcttctgg ccaaaagaca ggtggctttg tggaccctgg gacagttggt ggccagtact
     3481 ggctacgtgg tggagcccta caggaagtac cccactctgc ttgaagtgct gctgaatttt
     3541 ctgaagacgg agcagaacca gggcactcgg agagaggcca tccgagtgtt agggctcctc
     3601 ggggctttgg acccctacaa gcacaaagtg aacatcggca tgattgacca gtcccgagat
     3661 gcttctgctg tcagcctgtc agaatccaag tcaagtcaag attcctctga ctacagcacc
     3721 agtgaaatgc tggtcaacat gggaaacctg ccactggacg agttctaccc cgccgtgtcc
     3781 atggtggcct tgatgcggat cttccgagac cagtccctct ctcaccacca caccatggtg
     3841 gttcaggcca tcaccttcat cttcaagtcc ctggggctca agtgtgtgca gttcctgccc
     3901 caggtcatgc ccacgttcct taacgtcatc cgagtctgtg atggggccat ccgggaattt
     3961 ctgttccagc agctgggaat gctggtgtcc tttgtgaaga gccacatccg tccctacatg
     4021 gatgaaatag tcaccctcat gagagaattt tgggtcatga acacctcaat ccagagcaca
     4081 atcattcttc tcattgagca aatcgtggtg gctcttggag gtgaatttaa gctctacctg
     4141 ccccagctga tcccacacat gctgcgtgtc ttcatgcatg acaacagcca gggccgcata
     4201 gtctccatca agctgttagc agcgatccag ctgtttggcg ccaacctgga tgactatctg
     4261 cacttgttgt tgcctccgat cgtgaaattg tttgatgccc ctgaagttcc actgccgtcg
     4321 agaaaggcag cgttggagac agtggaccgc ctgacagagt ccctggattt cactgactat
     4381 gcctcccgca tcattcaccc gattgttcgc acgctagacc agagcccaga gctgcgctcc
     4441 acagccatgg acaccctgtc ttcacttgtg tttcaactag ggaagaagta ccagatcttc
     4501 attccaatgg tgaataaagt ccttgtgcga caccggatca atcaccagcg ctacgacgtg
     4561 ctgatctgca gaatcgtcaa ggggtacacg cttgctgatg aagaagaaga ccctttgatt
     4621 taccagcatc gaatgctaag gagcagccag ggagatgccc tggccagtgg accagttgaa
     4681 acaggaccca tgaagaaact gcatgtcagc accatcaacc tccaaaaggc ctggggagct
     4741 gccagaaggg tctccaagga cgactggctg gagtggctgc gacgcttgag tctggagctg
     4801 ctgaaggatt cctcatcacc ttccctgcgc tcatgctggg ccctggccca ggcctacaac
     4861 cccatggcca gggatctctt caacgctgcg tttgtgtcct gctggtctga actgaatgaa
     4921 gaccaacaag atgagctcat caggagcatt gagttggctc tcacttctca agacattgct
     4981 gaagtcacac aaaccctctt gaacttggct gagttcatgg agcacagtga caagggcccc
     5041 ctaccactga gagatgacaa tggcatcgtg ctgttgggtg agagagctgc caagtgccgg
     5101 gcatatgcca aagcactaca ctacaaagag ctggagttcc agaaggggcc cacgcctgcc
     5161 atacttgagt ccctcatcag cattaataat aaactgcagc agcctgaggc agcgtccggg
     5221 gtgttagagt acgccatgaa acacttcgga gagctggaga tccaggccac ctggtatgag
     5281 aagttgcatg agtgggagga cgcccttgtg gcctacgaca agaagatgga cacgaacaag
     5341 gatgacccag agctgatgct gggccgcatg cgctgtctcg aggccttggg agaatggggc
     5401 cagcttcatc agcagtgctg tgaaaagtgg actctggtta atgacgagac ccaggctaag
     5461 atggcccgga tggctgctgc agcagcatgg ggtttaggtc agtgggacag catggaggag
     5521 tacacctgta tgattcctcg ggatactcac gatggagcat tctacagagc agtgttggca
     5581 ctgcatcagg atctcttctc cttggctcaa cagtgcattg acaaggccag ggacctgctg
     5641 gacgccgagc tgactgccat ggcaggggag agctacagcc gagcctatgg ggccatggtt
     5701 tcttgccaca tgctgtccga gctggaggag gttatccagt acaaactcgt cccggagcga
     5761 cgggagatca tccgccagat ctggtgggag agactgcagg gctgccagcg tattgtagag
     5821 gactggcaga aaatcctcat ggtccggtcc cttgtggtca gccctcacga ggacatgaga
     5881 acctggctca agtacgcaag cctgtgtggc aagagcggca gactggctct tgctcataaa
     5941 accttagtgt tgctcttggg agttgatcca tctcggcaac ttgaccatcc tctgccaaca
     6001 gttcaccctc aagtgaccta tgcctacatg aaaaacatgt ggaaaagcgc tcggaagatt
     6061 gatgccttcc agcacatgca gcactttgtg cagaccatgc agcagcaggc ccagcacgcc
     6121 attgccacag aggaccagca gcacaagcag gagctgcata agctcatggc caggtgtttt
     6181 ctgaaacttg gggagtggca gctgaacctc cagggcatca acgagagcac catccccaag
     6241 gtgctacagt actacagtgc tgccacagag catgaccgca gctggtataa ggcttggcac
     6301 gcatgggcag tgatgaactt tgaagccgtg ctacactaca aacatcagaa ccaagcccgc
     6361 gatgagaaga agaaactgcg ccatgccagc ggggccaaca tcaccaatgc caccaccact
     6421 gccaccaccg ctgcctccgc tgccgctgcc accagcacag agggcagcaa cagtgaaagt
     6481 gaagccgaga gcaatgagag cagccccacc ccgtcccctc tgcagaagaa ggtcactgag
     6541 gatttgtcca aaaccctctt gttgtacact gtccctgctg tccaaggctt cttccgttct
     6601 atctccttgt cgagaggcaa caacctccag gatacactca gagtcctcac cttgtggttt
     6661 gattatggtc actggccaga tgtcaatgaa gccctggtgg aaggggtgaa ggccatacag
     6721 attgacactt ggttacaggt tatacctcag ctcattgcaa gaattgacac gcccagaccc
     6781 ttggtgggcc ggctcattca ccagctcctc acagatattg gtcggtacca cccacaggcc
     6841 ctcatctacc ccctgacggt ggcttctaag tctaccacca cagcccgtca caatgcagcc
     6901 aacaagatcc tgaagaacat gtgcgagcac agcaacacgc tagtccagca ggccatgatg
     6961 gtgagtgaag agctgattcg agtagccatc ctctggcatg agatgtggca tgaaggccta
     7021 gaagaggcct ctcgcttgta ctttggggag aggaacgtca aaggcatgtt tgaggtgctg
     7081 gagcccctgc atgctatgat ggaacgcggt ccccagaccc tgaaggaaac gtcctttaat
     7141 caggcatatg gtcgagattt aatggaggca caagaatggt gccgaaagta catgaaatca
     7201 gggaacgtca aggacctcac ccaagcctgg gacctctact atcacgtgtt cagacggatc
     7261 tccaagcagc taccacagct cacatccctg gagctgcagt atgtgtcccc caaacttttg
     7321 atgtgcagag accttgaatt ggctgtgcca ggaacatatg accccaacca gccaatcatt
     7381 cgcattcagt ccatagcccc gtctttgcaa gtcatcacat ccaagcagag gcctcggaag
     7441 ctgaccctga tgggcagcaa tgggcacgag tttgttttcc tcctgaaagg ccatgaagat
     7501 ctgcggcagg acgagcgagt gatgcagctc tttggcctgg tgaacacact cctagccaat
     7561 gacccaactt ctcttcgaaa gaacctcagc atccagagat acgccgtcat tcctctgtcc
     7621 accaactcgg gcctgattgg ctgggtgccc cactgtgaca cgctgcatgc cctcatccgg
     7681 gactacagag agaagaagaa gatcctgctg aacatcgagc accgcatcat gctgcggatg
     7741 gctcctgact atgaccacct gactctgatg cagaaggtgg aggtgtttga gcatgctgtc
     7801 aacaacacag ccggggatga cctggccaag ctgctgtggc tgaaaagccc cagctcagag
     7861 gtgtggtttg accgaagaac caattatact cgctccctgg ctgtcatgtc catggttgga
     7921 tacattttag gccttggaga caggcaccca tccaacctga tgctggaccg gctgagtgga
     7981 aagatcctgc acattgactt tggggactgc tttgaggttg ctatgaccag agagaaattt
     8041 ccagaaaaga ttccatttag actaacaaga atgttgacca atgctatgga ggttaccggt
     8101 ctcgatggca actatagaac cacatgccac acagtgatgg aggtgcttcg ggagcacaag
     8161 gacagtgtca tggctgtgct aaaagccttt gtctatgacc ctctgctgaa ttggaggctg
     8221 atggacacaa atgccaaagg caacaagcgg tcccgaacca ggacagactc ctattctgca
     8281 ggccagtcag tagaaatttt ggacggtgta gaacttggag aaccagccca taagaaaaca
     8341 gggaccactg tgccagaatc catccattct ttcattggag atggtttggt gaaaccagaa
     8401 gccttaaaca agaaagctat tcagattatt aacagggttc gagataagct cactggtcgg
     8461 gatttctctc atgatgacac tttggatgtt ccaacccaag tggaactgct tatcaagcaa
     8521 gcgacatctc atgagaacct ctgccagtgc tacattggct ggtgtccttt ctggtaagcg
     8581 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     8641 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     8701 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     8761 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8821 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8881 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9121 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9241 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9301 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     9361 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9421 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9541 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9601 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     9661 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     9721 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     9781 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     9841 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     9901 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     9961 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
    10021 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
    10081 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
    10141 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
    10201 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
    10261 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
    10321 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    10381 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    10441 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    10501 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
    10561 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
    10621 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    10681 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    10741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10861 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    10921 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10981 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    11041 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    11101 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11161 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    12001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    12061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    12121 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    12181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    12241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    12301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    12361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    12421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    12481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    12541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    12601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    12661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    13021 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
