LOCUS       sequence-480233-        7175 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  5' AAVLINK plasmid for CHD2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Zhonghua Lu Lab
  TITLE     Zhonghua Lu AAV Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' CHD2
FEATURES             Location/Qualifiers
     source          1..7175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      4653..5108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4740..4759)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4950..4971
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5125..5144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5244..5266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5304..5322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5390..5494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5495..5563,5564..6355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5713..5732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6526..7114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7015..7034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctagaa ataaggacaa aagccaagag gaggacagtt cgctacacag
      781 caatgcatcg agtcactcag cctctgaaga agcttcgggt tcagactcag gcagtcagtc
      841 ggaaagtgag cagggaagtg atccaggaag tggacatggc agcgagtcga acagcagctc
      901 tgaatcttct gagagtcagt cggaatctga gagcgaatca gcaggttcca aatcccagcc
      961 agtcctccca gaagccaaag agaagccagc ctctaagaag gaacggatag ctgatgtgaa
     1021 gaagatgtgg gaagaatatc ctgatgttta tggggtcagg cggtcaaacc gaagcagaca
     1081 agaaccatcg cgatttaata ttaaggaaga ggcaagtagc gggtctgaga gtgggagccc
     1141 aaaaagaaga ggccagaggc agctgaaaaa acaagaaaaa tggaaacagg aaccctcaga
     1201 agatgaacag gaacaaggca ccagtgcaga gagtgagcca gaacaaaaaa aagtaaaagc
     1261 cagaagacct gtccccagaa gaacagtgcc caaacctcgt gttaaaaagc agccgaagac
     1321 tcagcgtgga aagagaaaaa agcaagattc ttctgatgag gatgatgatg atgacgaagc
     1381 tcccaaaagg cagactcgtc gaagagcggc taaaaacgtt agttacaaag aagatgatga
     1441 ctttgagact gactcagatg atctcattga aatgactgga gaaggagttg atgaacagca
     1501 agataatagt gaaactattg aaaaggtctt agattcaaga ctgggaaaga aaggagccac
     1561 tggagcatct actactgtat atgcgattga agctaatggc gaccctagtg gtgactttga
     1621 cactgaaaag gatgaaggtg aaatccagta cctcatcaag tggaagggtt ggtcttacat
     1681 ccacagcaca tgggagagtg aagaatcctt acagcaacag aaagtgaagg gcctaaaaaa
     1741 actagagaac ttcaagaaaa aagaggacga aatcaaacaa tggttaggga aagtttctcc
     1801 tgaagatgta gaatatttca attgccaaca ggagctggct tcagagttga ataaacagta
     1861 tcagatagta gaaagagtaa tagctgtgaa gacaagtaaa tctacattgg gtcaaacaga
     1921 ttttccagct catagtcgga agccggcacc ctcaaatgag cccgaatatc tatgtaaatg
     1981 gatgggactc ccctattcag agtgtagctg ggaagatgaa gccctcattg gaaagaaatt
     2041 ccagaattgc attgacagct tccacagtag gaacaactca aaaaccatcc caacaagaga
     2101 atgcaaggcc ctgaagcaga gaccacgatt tgtagcttta aagaaacaac ctgcatattt
     2161 aggaggggag aatctggaac ttcgagatta tcagctagaa ggtctaaact ggctagctca
     2221 ttcctggtgc aaaaataata gtgtaatcct tgctgatgaa atgggcctag gaaagaccat
     2281 ccagaccata tcattcctct cctacctgtt ccaccaacac cagctgtatg gcccctttct
     2341 tatagtcgtc cctttatcca ccctcacctc atggcagaga gagtttgaaa tctgggcacc
     2401 agagattaac gtagtggttt acataggtga cctgatgaac agaaatacga tacgggaata
     2461 tgaatggatt cattcccaaa ccaaaagatt gaagttcaac gcacttataa caacatatga
     2521 gatcctcttg aaagataaga ctgtgctggg cagtattaac tgggcctttc tgggagtgga
     2581 tgaagcccat cggttgaaga atgatgactc tttattgtat aaaactctga ttgatttcaa
     2641 gtccaaccat aggctcctga ttacggggac ccctcttcag aattccctca aagagctctg
     2701 gtccttgctg cactttatta tgccggagaa gtttgaattt tgggaagatt ttgaagaaga
     2761 ccatgggaag gggagagaga atggctacca gagtcttcat aaggtgctag agcctttcct
     2821 tctccggaga gtcaaaaaag atgtggagaa atcccttcct gctaaagtgg aacagattct
     2881 cagggtggag atgtcagccc ttcagaaaca gtattacaag tggattctga ccaggaatta
     2941 caaggctctt gccaaaggaa caagaggcag cacatctggt tttcttaata ttgtgatgga
     3001 actgaaaaaa tgttgcaacc actgctatct gattaaaccc cctgaagaaa atgaaaggga
     3061 aaatggacag gagattcttc tgtccctcat aaggagcagt gggaagttga ttttattaga
     3121 caaactgttg acaagacttc gagaaagggg gaatcgagtg cttatcttct ctcagatggt
     3181 gagaatgttg gatatcctgg ctgaatacct aactattaaa cactatcctt tccagcgtct
     3241 ggatggttcc atcaagggag aaatccgaaa acaggcactg gaccacttca atgcagatgg
     3301 gtctgaggac ttctgtttcc tgctctcgac aagggctggt ggcctgggaa tcaatttggc
     3361 ttcagcggac acagtcgtca tctttgactc tgactggaac ccccagaatg acttgcaggc
     3421 acaagcccga gcgcatagaa ttggtcagaa gaagcaggta aatatttacc gcttagttac
     3481 aaaggggact gtggaggagg agatcataga acgggccaaa aagaagatgg tattagatca
     3541 tctggtgatt cagcgcatgg acaccactgg ccggacgatc ctggaaaaca actcaggaag
     3601 gtccaactca aatcctttta ataaagaaga gctgacagct attttgaaat ttggagcaga
     3661 ggatctcttc aaagaactgg aaggggagga atcagaacct caggaaatgg atatagatga
     3721 aattttgcgg ttggctgaaa cgagagagaa tgaagtgtca acaagtgcaa cagatgaact
     3781 tctatcacag tttaaggttg ccaactttgc aacaatggaa gatgaagaag agctagaaga
     3841 gcgtcctcac aaggactggg atgagatcat tccagaggaa caaaggaaaa aagtagagga
     3901 ggaagagcgg cagaaggagc tagaagaaat ttatatgctg cctcgaattc ggagttccac
     3961 taaaaaggct cagacaaatg acagtgactc tgacactgag tctaagaggc aggcccagag
     4021 atcctctgct tctgagagtg aaacggaaga ctctgatgat gacaagaagc caaagcgcag
     4081 agggcgtccg aggagtgtgc ggaaggacct cgtggaggga tttactgatg cagagatccg
     4141 aaggttcatc aaggcttata agaagtttgg tctccctctt gaacggctgg agtgcatagc
     4201 acgtgatgct gagctggtag ataagtcggt ggcagatctg aagcgcctgg gtgaactgat
     4261 ccacaacagc tgtgtgtcag caatgcagga atacgaagag cagctgaaag aaaatgccag
     4321 cgagggaaaa ggaccaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag
     4381 ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac
     4441 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4501 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc ggcctcagtg agcgagcgag
     4561 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4621 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4681 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4741 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4801 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4861 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4921 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4981 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     5041 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     5101 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5161 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5221 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5281 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5341 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5401 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5461 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5521 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5581 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5641 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5701 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5761 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5821 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5881 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5941 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6001 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6061 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6121 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6181 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6241 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6301 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6361 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6421 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6481 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6541 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6601 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6661 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6721 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6781 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6841 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6901 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6961 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7021 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7081 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7141 gttcctggcc ttttgctggc cttttgctca catgt
//
