LOCUS       sequence-480235-       18808 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Cas12i1-KRAB_SRDX system backbone for gene regulation in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   He Y, Liao S, Ren Q, Tang X, Zheng X, Qi Y, Zhang Y
  TITLE     CRISPR-Cas12i confers efficient genome editing and gene regulation 
            in plants.
  JOURNAL   Plant Physiol. 2025 Apr 30;198(1):kiaf125. doi: 
            10.1093/plphys/kiaf125.
  PUBMED    40305671
REFERENCE   2  (bases 1 to 18808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-04-30"; volume: "198(1):kiaf125. doi"; pages:
            " 10.1093/plphys/kiaf125"
COMMENT     Sequence Label: pGEL1021
FEATURES             Location/Qualifiers
     source          1..18808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(38..105)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(547..583)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     terminator      complement(2147..2395)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(2406..2426)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2439..2459)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2472..2492)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2844..2891)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(6192..6212)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6215..6224
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8263..8279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8263..8279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8276..8298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8287..8303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8311..8317,8318..8335,8336..8341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8356..8377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8568..9244
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9181..9201
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9311..10336
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             9311..10336
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10377..10551
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10629..10653
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11078..11872
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13297..13885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13786..13805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14039..14056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14071..14211
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14137..14156)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14297..14319
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14555..14749
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14815..15888)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16317..16946)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    18247..18271
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     18459..18481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18473..18490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     18474..18490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccaatcca gaagatggac aagtctagga ctcgagtgtc ccattcgcca tgccgaagca
       61 tgttgcccag ccggcgccag cgaggaggct gggaccatgc cggcctgaga ccaagccaga
      121 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
      181 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca
      241 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac
      301 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga
      361 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag
      421 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      481 ttgtggatgt acaagagtga tattattgac acgcccggtc tccgtgccaa cgatgggcac
      541 aaaaatgacg agcttactcg tttcgtcctc acggactcat cagatttttc ctgcaggctg
      601 cacacacaat taccgaagca tggtcagcaa ctatgaacat gaaattataa caataccaag
      661 caagtacagc agaaatcagt caatcaaatt attccaatat gttagataca cgatctaaac
      721 cacaataaca tcagtgtacc tatcaacacc aacagtgacc taacaaaata gagagtcgca
      781 ccgtgctcga gttcacaaaa ataatctgca ggtctatgaa ctactagaat tccatcggca
      841 aactgattca ataactacta gcactatccc gagagcacat aaggcaggtt aattaacata
      901 ggctagaatt tacaggtgca acagttgata agataggcat aagcaagtaa aatatcctga
      961 aagctatgct atgtaatcac taatcaacaa gacgcagccc ttagattaaa catgaacaga
     1021 gtagaatcta tgcaactaga gggactgaat caaaaacaac ctaagcaaaa tatacctgaa
     1081 gctgctactt ggatcaacga gaaaaacaga gactaaatcc tactgattga acagatcgga
     1141 acaaatcaca accctagcac agttctactt ctagatccga accaataaat acttggacca
     1201 cgaacagcat cgctcataat cacagatcca gcataccacg cgaaccccat ctaaccctgt
     1261 tcaaatctac cacagatcgc aaaacggaga ctaacaacaa catctcacaa tcacgaacag
     1321 gccgggaaat caaatcaaac gcgaggaacg gagaggatca gaaaatcgca taccttgaga
     1381 aggcgattcg acgcttcgct tcgaggggag attttttttt ctctgcgatt ttggggattc
     1441 gattgatgga ggaagaggcg aggagggggt ggggtattta taggtgggga gggggggact
     1501 cgtgagtggg cccaggggtg cggtgggtgg ggcccagtga gaatatagac aaataaagag
     1561 agggagaggc ggtttgggga gtccgtcccc accacgcaaa cgcgagaagg aaagaaagga
     1621 acggaaaggg ggcgggtcca gacggcgccg cggagatatc gaggaggtgg aaagctgatg
     1681 ccgtttgctc acggcggggt tggtggccgt taacacgcag cgaagcggcg ttactgccgt
     1741 gtacgttttg tgacctttcg aaagagagcc ccagcccaag aaaggctggc caggtttttt
     1801 tgtactgtgt acgattacga cggaagaaaa catactcgtt gttttgtatt ggtagatact
     1861 gggccgtagt aaaattagaa tcagttgtta attggttatt atatatacca tcttaagcta
     1921 ccaaaattaa taaagttgtg atggctatgg tatattttgg tagtgaatta tattgatttg
     1981 gtatatatta gaatcaatcc aaccacaaca caaccaaaaa tacctgaagc tcaatattga
     2041 tggtgctttt cgactatcta gcaaatgtgg tgaatggggc tttataattg aagcagccca
     2101 ggatgcactt tgtgcagaga ctgatgtggg cgcgccgtcc gagctcctta tctttaatca
     2161 tattccatag tccataccat agcacataca gtagttatat gctgcagaag agatccaaca
     2221 aaacattcac aatggattat agaaacattt gtttattcat tataatgtga tcttacattc
     2281 atttaatatt agaaaaagcc acaaattcat aacacaacaa gccaagaaaa aaacacaaac
     2341 ttaagcacac aagcttttta tttgacacac caaatatttc atcttcatct tcatatctcg
     2401 agtcacacct tgcgcttctt cttcggatca gctctagaca ccttccgctt cttctttgga
     2461 tctgccctag aaaccttcct cttcttctta ggatcagccc ttgaagcgaa gccaagccgc
     2521 agctccaggt cgaggtcgag ggcaaacccc aggcggagtt ccaaatcaag gtccagggcg
     2581 aagccgagcc tcaactcaag atccaggtcg agggacccgc cgctggaacc aaccagccat
     2641 ggttcctctc ctttctccaa cctaagaatg acatccggct tggtcagctg gtagccaagt
     2701 gacaccaaat tcttatagtt ttcgagcata acgttgcggt acaggatttg ctgggccgtg
     2761 tcgaggagct tccactcctc cctggtaaaa tcgacgaaca cgtccttgaa ggtcaccagg
     2821 gttctggacc cgccggaact gcccttcttt ttcttagcct gtccggcctt tttggtggca
     2881 gcaggacgct tttttttctt gaaatccttt tggatatcca caagcccgac attgtacgcg
     2941 gcgacgaggt cagcattgca cacccaaaat tgttttccgt tccagttaac ggacgtggcg
     3001 tcagcatcaa gcttgtacgt agccaaataa aacatccccc cacgtgacgg gaataggagc
     3061 tgatcctctg atcgttggtg gagaatattt gccatgatct gtttgaattt ctcgaggcta
     3121 acaccgagga cgtcgttctt tttcaggcca taggtagcga gaaacgccat ggcgccttca
     3181 ttgtaatatg cattggtcgg cctcttactc ggcttatcgc taagaaagct caggatctct
     3241 ttccggttct tttcggtgag ctcggagggt gtacatctcg aataccgtgc ccggaatgtg
     3301 ttttcagggt tcttgtgcac aaagggatcc tgatggctcg tgaaattagg gttgacctcc
     3361 acaaactcca gaccctggtg catcatcacc atttccttca ccttattggc tacgccgcgc
     3421 gctagccaat ccatcaggaa agaattagta cttgattttt tccccttctt ggtggtatca
     3481 ctaatgtttt cgcctttgat gagaactggg ccgtacttgt cacgcaattc aagtgctttc
     3541 gcgacaatct tgttagctat cacctccttt ttactcttga ctttattcaa cctcttttct
     3601 tctagcgcaa gtctcaaagc aaacatctcc ggatctgcct tctgtttatc ctcatctgtg
     3661 ataggaggcg ccttaacgtc ggattttgaa ttctttggct tctttaacag gtgcccgaag
     3721 tatgtcccga tgaggctctt agccaccttg aacataacaa agcttaggtt tgacaaggaa
     3781 gagagcttga ggacggaaag tttcccatcg cggagcagct cgaatatctc ctccctgtac
     3841 tccttagcct gcgagctgtg gttacgtatg gatgattgca gtagcttaca atacttcatg
     3901 ttaaagtaat acaatgaagt ctcatcatcg gcgatggcct caaaatcctt catgaagtca
     3961 atctgaatgt tgttaccgcg gttatctttc atcgtaccat ttctatattt ctcgagaaag
     4021 gacctgcgac tttcgacatg tggtttgcag tagaggagct tgacgccatt gtatgagagc
     4081 tgatcgtaac ttttgtcgcg aacttgtgac tctacggtca cgaaacctga ttcaatgggc
     4141 tttaccggca ggtcattgta gtcaattacc ccatcaccgt tcgcgtgtgc ttcaattgca
     4201 gcatatgtat tctttctaac tatatttgcg tcataaccga gcacaacttt ataattcttt
     4261 tccttctttt ttttcacttt tgtggcgagt gtgacttcaa agttgttgcc cctcttgcat
     4321 atattaatgt tgaaatcctt tccccacgta taacgcggca gcagattttg ttgccgcgcg
     4381 gcttccaggc gcatttgcct ctttttaacc ttcctcaagc cgactggcgc cgaacgtatc
     4441 tgttcaattt gctctgcaga aagcgcaggc ttgccctcgt agccgtttgt cttagtcctg
     4501 aaccgggccg ccagtgcgtc cggcggattt tcggaagtcg ccgggtagta gacctcttcc
     4561 aagaatcgtg tggaagacaa cgcgtagtgg tgtttttccc atctcatggt tttcgtattc
     4621 agtaccttta tttcgatcca aatgtagtta tcaacctgat ttctgttaca tttaagatta
     4681 tgtttaagct tgtccttggg cgagataatt tttcccagca acttagagtt gttcccgaaa
     4741 ttaaagctag ggtacttctg aataaccggg ttaatcttct gcttctcaac cttatgcttc
     4801 ttagacagaa aggtgatgcc atcgatgatc ttgtcgacgg cgatgtcgtc cttgcgagag
     4861 tagaggtacc tcaacagttg cactagcgga gttttcgcct ccttgcccac ctcctcgcaa
     4921 taagtgttga tggtctggca cacgtccccc tggtacactt tgattcgctt ttccttctta
     4981 tcttcggata gggatgcaat atcctcttca tacttcgaat ggatcgcgtt taaatcctcc
     5041 cataaagtct tgatattact ctcgccgatg tgatgagggt gcagcaccca cttaacattg
     5101 gtgtcgctct cgaagtatgg ggagtcgaaa aatccttcga cgatcttgcc gaagggctca
     5161 ttctcatatt tgatatcatt cttagcgtcg atttgcttaa gtacgaactt acaatttttg
     5221 gtggtcatgc ccttaattgg gctcaaggcg ttctccagca tggcactata cgagccgaga
     5281 tagtatggtc ccaccttgga tgcaagaaac gcttttatct tctccatgca cttccactca
     5341 cactcatatt cgaaccgacc aagcttctct ttgaggttag ctatgaggct tgggacgttt
     5401 acctcgtcgt catggttggt ccctagctcg taatcttgca ctatcttgag aattgatggc
     5461 gtggcgccct tgcgtgagca tcggatgcgg agcttccggt agctgtcgca gtcaccagcc
     5521 ttcaaaatga tcgaggctaa ttgctccacg gaattaatct gtgttgattc ctccagaagt
     5581 tggattacct tgctgtagaa ctggtgctta gtttgcctat tattctttgc tcctgttccg
     5641 aacatgaccg acgtaatgta gtgggccgtc gagagagaca atccaatgca tttttccttg
     5701 tacatcaact tcagatcatg atgcatgtca gaccggctga gctgcaactc cgccagaagc
     5761 ctctcatatt caaccctcat atctttccac tgatgtttct ctgtatcaaa gttactggcg
     5821 aaatactcct gggcttggat gtcaggttca gatccactgt actgctggaa caagttagcc
     5881 aagttcgagg gagaaacgcc ctgcggttta gaatttattg gcatgacttt gaaccaattc
     5941 acggcaagaa ggaggtcatc agaaatcttg tccttgtttg cagtcccgga gattatgtcg
     6001 cggtcgatgc ctccgccaaa ctggttccac agttcgaaga agataggcgt accatccata
     6061 agatagtcca tgaagttacc gagcagcttc attctatcat gtgaccgcgg tatcatctta
     6121 gttgtgtaag cttttctagt ctcgctcgca tttttctcct tattagaagc cgcaggcacc
     6181 ccgtgaatac caaccttccg cttcttctta ggagccatgg cggcctgcag gaagtaacac
     6241 caaacaacag ggtgagcatc gacaaaagaa acagtaccaa gcaaataaat agcgtatgaa
     6301 ggcagggcta aaaaaatcca catatagctg ctgcatatgc catcatccaa gtatatcaag
     6361 atcaaaataa ttataaaaca tacttgttta ttataataga taggtactca aggttagagc
     6421 atatgaatag atgctgcata tgccatcatg tatatgcatc agtaaaaccc acatcaacat
     6481 gtatacctat cctagatcga tatttccatc catcttaaac tcgtaactat gaagatgtat
     6541 gacacacaca tacagttcca aaattaataa atacaccagg tagtttgaaa cagtattcta
     6601 ctccgatcta gaacgaatga acgaccgccc aaccacacca catcatcaca accaagcgaa
     6661 caaaaagcat ctctgtatat gcatcagtaa aacccgcatc aacatgtata cctatcctag
     6721 atcgatattt ccatccatca tcttcaattc gtaactatga atatgtatgg cacacacata
     6781 cagatccaaa attaataaat ccaccaggta gtttgaaaca gaattaattc tactccgatc
     6841 tagaacgacc gcccaaccag accacatcat cacaaccaag acaaaaaaaa gcatgaaaag
     6901 atgacccgac aaacaagtgc acggcatata ttgaaataaa ggaaaagggc aaaccaaacc
     6961 ctatgcaacg aaacaaaaaa aatcatgaaa tcgatcccgt ctgcggaacg gctagagcca
     7021 tcccaggatt ccccaaagag aaacactggc aagttagcaa tcagaacgtg tctgacgtac
     7081 aggtcgcatc cgtgtacgaa cgctagcagc acggatctaa cacaaacacg gatctaacac
     7141 aaacatgaac agaagtagaa ctaccgggcc ctaaccatgg accggaacgc cgatctagag
     7201 aaggtagaga gggggggggg ggaggacgag cggcgtacct tgaagcggag gtgccgacgg
     7261 gtggatttgg gggagatctg gttgtgtgtg tgtgcgctcc gaacaacacg aggttgggga
     7321 aagagggtgt ggagggggtg tctatttatt acggcgggcg aggaagggaa agcgaaggag
     7381 cggtgggaaa ggaatccccc gtagctgccg gtgccgtgag aggaggagga ggccgcctgc
     7441 cgtgccggct cacgtctgcc gctccgccac gcaatttctg gatgccgaca gcggagcaag
     7501 tccaacggtg gagcggaact ctcgagaggg gtccagaggc agcgacagag atgccgtgcc
     7561 gtctgcttcg cttggcccga cgcgacgctg ctggttcgct ggttggtgtc cgttagactc
     7621 gtcgacggcg tttaacaggc tggcattatc tactcgaaac aagaaaaatg tttccttagt
     7681 ttttttaatt tcttaaaggg tatttgttta atttttagtc actttatttt attctatttt
     7741 atatctaaat tattaaataa aaaaactaaa atagagtttt agttttctta atttagaggc
     7801 taaaatagaa taaaatagat gtactaaaaa aattagtcta taaaaaccat taaccctaaa
     7861 ccctaaatgg atgtactaat aaaatggatg aagtattata taggtgaagc tatttgcaaa
     7921 aaaaaaggag aacacatgca cactaaaaag ataaaactgt agagtcctgt tgtcaaaata
     7981 ctcaattgtc ctttagacca tgtctaactg ttcatttata tgattctcta aaacactgat
     8041 attattgtag tactatagat tatattattc gtagagtaaa gtttaaatat atgtataaag
     8101 atagataaac tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat tttttataac
     8161 ttagacatgc aatgctcatt atctctagag aggggcacga ccgggtcacg ctgcactgca
     8221 ggcgcgccga tccccgggta ccgagctcga attcgtaatc atgtcatagc tgtttcctgt
     8281 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8341 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8401 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8461 aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga cactctcgtc
     8521 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
     8581 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     8641 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
     8701 gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     8761 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa
     8821 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     8881 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     8941 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     9001 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
     9061 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     9121 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     9181 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg
     9241 ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca gatccggggg
     9301 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     9361 aaagttcgac agcgtgtccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     9421 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     9481 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     9541 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     9601 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     9661 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     9721 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     9781 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     9841 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     9901 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     9961 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
    10021 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
    10081 aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
    10141 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
    10201 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
    10261 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
    10321 gagggcaaag aaatagagta gatgccgacc gggatctgtc gatcgacaag ctcgagtttc
    10381 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
    10441 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
    10501 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
    10561 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
    10621 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
    10681 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
    10741 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
    10801 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
    10861 aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata
    10921 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
    10981 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
    11041 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
    11101 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
    11161 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
    11221 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
    11281 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
    11341 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
    11401 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
    11461 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
    11521 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
    11581 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
    11641 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
    11701 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
    11761 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
    11821 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
    11881 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgctga tgaatcccct
    11941 aatgattttt atcaaaatca ttaagttaag gtagatacac atcttgtcat atgatcaaat
    12001 ggtttcgcca aaaatcaata atcagacaac aaaatgtgcg aactcgatat tttacacgac
    12061 tctctttacc aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct
    12121 tgccacgcct tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca
    12181 accaaagcga gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac
    12241 ctccacaaag agcgactcgc tgtataccgt tggcatgcta gctttatctg ttcgggcaat
    12301 acgatgccca ttgtacttgt tgactggtct gatatccgtg agcaaaaacg gcttatggta
    12361 ttgcgagctt cagtcgcact acacggtcgt tctgttactc tttatgagaa agcgttcccg
    12421 ctttcagagc aatgttcaaa gaaagctcat gaccaatttc tagccgacct tgcgagcatt
    12481 ctaccgagta acaccacacc gctcattgtc agtgatgctg gctttaaagt gccatggtat
    12541 aaatccgttg agaagctggg ttggtactgg ttaagtcgag taagaggaaa agtacaatat
    12601 gcagacctag gagcggaaaa ctggaaacct atcagcaact tacatgatat gtcatctagt
    12661 cactcaaaga ctttaggcta taagaggctg actaaaagca atccaatctc atgccaaatt
    12721 ctattgtata aatctcgctc taaaggccga aaaaatcagc gctcgacacg gactcattgt
    12781 caccacccgt cacctaaaat ctactcagcg tcggcaaagg agccatggat tctagcaact
    12841 aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg
    12901 cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat
    12961 agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa
    13021 ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag
    13081 gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg
    13141 cggcattctg gctacacaat aacaagggaa gacttactcg tggctgcaac cctactagct
    13201 caaaatttat tcacacatgg ttacgctttg gggaaattat gaggggatct ctcagcactg
    13261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    13321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    13381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    13441 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    13501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    13561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    13621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    13681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    13741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    13801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    13861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    13921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    13981 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    14041 cgagtcagtg agcgaggaag cggaagaggg cctgatgcgg tattttctcc ttacgcatct
    14101 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
    14161 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
    14221 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    14281 caagctgtga ccgtttccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    14341 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
    14401 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
    14461 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
    14521 ttgcagctct tgggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
    14581 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
    14641 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
    14701 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagaggcc
    14761 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
    14821 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
    14881 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
    14941 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
    15001 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
    15061 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
    15121 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
    15181 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
    15241 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
    15301 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
    15361 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    15421 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    15481 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    15541 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    15601 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    15661 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    15721 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    15781 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    15841 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    15901 ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc
    15961 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    16021 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    16081 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    16141 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    16201 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    16261 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    16321 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    16381 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    16441 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    16501 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    16561 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    16621 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    16681 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    16741 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    16801 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    16861 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    16921 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    16981 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    17041 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    17101 catcagacaa accggccagg atttcatgca gccgcacggt tgagccgtgc gcgggcggct
    17161 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    17221 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    17281 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    17341 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    17401 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    17461 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    17521 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    17581 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    17641 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    17701 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    17761 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    17821 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    17881 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    17941 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    18001 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    18061 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    18121 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    18181 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    18241 tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga
    18301 aacgacaatc tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg
    18361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    18421 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    18481 gacggccagt gccacactga gctcgatatc ggcgcgcccc gggagctcgc aaaacacacc
    18541 tagactagat ttgttttgct aacccaattg atattaatta tatatgatta atatttatat
    18601 gtatatggaa ttggttaata aaatgcatct ggttcatcaa agaattataa agacacgtga
    18661 cattcattta ggataagaaa tatggatgat ctctttctct tattcagata attagtaatt
    18721 acacataaca cacaactttg atgcccacat tatagtgatt agcatgtcac tatgtgtgca
    18781 tccttttatt tcatacatta attaactt
//
