LOCUS       sequence-480861-        5349 bp    DNA     circular SYN 28-JUL-2025
DEFINITION  Mouse Rps7 fused to meGFP for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5349)
  TITLE     Stemness factor Mex3a times translation and protein trafficking to 
            ensure robust differentiation of olfactory sensory neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV-Rps7-meGFP
FEATURES             Location/Qualifiers
     source          1..5349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        286..489
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     440..460
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1222..1935
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(1261..1282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1522..1541)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1869..1890
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2058..2179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2095..2114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2149..2168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2186..2641)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2323..2344)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2535..2554
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2668..2772
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2774..3131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2774..2965
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2797..2817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2982..3117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3166..3960
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3166..3960
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3220..3239)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3830..3849
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4148..4167)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4192..4239
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4568..5156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5057..5076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        join(5331..5349,1..285)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
       61 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      121 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      181 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      241 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      301 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      361 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      421 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      481 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
      541 tcaagcttcg aattcatgtt cagctcgagc gccaagatcg tgaagcccaa tggcgagaag
      601 ccggacgagt tcgagtctgg catctctcag gcgctgctcg agctggagat gaactccgat
      661 ctcaaggcgc agctgcgtga actcaacatc accgcagcca aggaaatcga agttggtggt
      721 ggtcggaaag ctatcataat ttttgtacca gttcctcagc ttaaatcttt ccagaaaatc
      781 caagtccggc tagttcgtga attggagaaa aagttcagtg gcaagcacgt ggtcttcatt
      841 gctcagagga ggattctgcc caagccaacc cggaaaagcc gtacgaaaaa taagcagaag
      901 cgccccagaa gccgtaccct gacagcagtg cacgacgcca tccttgagga cttagtcttc
      961 ccaagtgaaa ttgtgggtaa gaggatccgt gtgaaactgg atggcagccg gctcataaaa
     1021 gttcacttag acaaagcaca gcagaacaac gtggaacaca aggttgaaac tttttctggt
     1081 gtgtacaaga agctcacagg caaggatgtt aattttgaat tcccagagtt tcagttggaa
     1141 ttcggaggcg gaggttctgg gggaggaggt agtggcggtg gtggttcagg aggcggcgga
     1201 agcggtggag gtggaagcgg tagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1261 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1321 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1381 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1441 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1501 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1561 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1621 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1681 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1741 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1801 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     1861 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1921 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     1981 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2041 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2161 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     2221 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2281 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2341 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2401 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2461 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2521 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2581 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2641 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2701 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2761 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     2821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2881 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2941 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3001 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3061 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3121 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3181 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3241 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3301 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3361 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3421 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3481 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3541 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3601 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3661 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3721 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3781 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3841 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3901 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3961 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4021 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4081 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     4141 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     4201 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4261 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4321 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4381 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     4441 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4501 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4561 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4621 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4681 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4741 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4801 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4861 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4921 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4981 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5041 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5101 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5161 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5221 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     5281 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5341 cttacggta
//
