LOCUS       sequence-480863-        5093 bp    DNA     circular SYN 28-JUL-2025
DEFINITION  Expression of Mex3a-mCherry fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5093)
  TITLE     Stemness factor Mex3a times translation and protein trafficking to 
            ensure robust differentiation of olfactory sensory neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV-Mex3a-mCherry
FEATURES             Location/Qualifiers
     source          1..5093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        167..546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        547..750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     701..721
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          842..974
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             2578..3288
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2723..2741)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2991..3010
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3295..3314)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    3324..3405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3349..3368
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3603..4191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3683..3702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4272..5066)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4272..5066)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4383..4402)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4993..5012
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgtctcgtcc cggtctcctc ccatgcatgt caatattggc cattagccat attattcatt
       61 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
      121 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttggca ttgattattg
      181 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      421 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      481 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      541 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
      601 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      661 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
      721 gtacggtggg aggtctatat aagcagaggt cgtttagtga accgtcagat cactagtagc
      781 tttattgcgg tagtttatca cagttaaatt gctaacgcag tcagtgctcg actgatcaca
      841 ggtaagtatc aaggttacaa gacaggttta aggaggccaa tagaaactgg gcttgtcgag
      901 acagagaaga ttcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc
      961 ctttctctcc acaggggtac catgcctagt ctagtggtat ctggaataat ggaaagaaat
     1021 gggggctttg gagaactagg atgtttcggg ggaagcgcta aagacagggg gcttctggaa
     1081 gacgagcgcg ccctccagct ggcactggac cagctctgcc ttctggggct gggcgagccg
     1141 cctgcaccca ccgccggaga agatggtggg ggagggggcg gcggagcacc agcccagcct
     1201 gcagcaccac ctcagccagc tccccctcct cctccagcag ccgctcccgc tgctccaagc
     1261 gcagcccctg ccgctcaaac cccacaacct cccacagccc ctaagggcgc aaacgatgcc
     1321 aagctgtgtg ccctgtataa agaggcagaa ttgaggctta aaggaagctc aaacaccacg
     1381 gagtgcgttc ccgtgcccac ctccgagcac gtggccgaga tcgtgggcag gcaaggctgc
     1441 aagattaagg ctctgagggc caaaaccaac acctacatca agacacctgt gcgaggtgag
     1501 gagccagtgt tcatggtgac tgggaggcgg gaagacgtgg ccacagcccg gcgggagatc
     1561 atctctgcag ctgagcactt ctccatgata agagcctcac gcaacaagtc tggcgccgcc
     1621 tttggcgtgg cccctgctct gccaggccaa gtgaccatcc gtgtacgagt accctaccgc
     1681 gtggtgggac tggtggtggg ccccaaggga gcgaccatca aacgcatcca gcagcagacc
     1741 aacacgtaca tcatcactcc cagccgggac cgcgaccccg tgtttgagat aaccggcgca
     1801 ccgggcaacg tggagcgtgc gcgggaggag atcgagacgc acatcgctgt gcgcaccggc
     1861 aagattcttg aatacaacag cgacggcgac ttcctggctg gcagccctga cgctgccttg
     1921 gacagccgct actcagacgc ctggcgggtg catgctccag gctgtaagcc gctctccacc
     1981 ttcaggcaga acagcctggg ctgcataggc gaatgcggcg tggactcggc ctttgaggcc
     2041 ccgcgcctaa gcgagcaggg cggggacttt ggctacagcg gatatctgtt tccgggctac
     2101 ggtgtgggca agcaggacgt gtactatggc gtggcagaga caagcccccc gctgtgggcg
     2161 ggtcaggaga atgccacgcc cacctcggtg ctcttctcct ctgcctcctc ctcctcctct
     2221 tcagccaagg cacgcgcggg ccccccaggc gcgcatcgat ctcctgccac ctccgcgggg
     2281 ccagagcagc tgaccgggct ccctcggagg ccacccggag agccactgca gggtttctct
     2341 aaactgggga caggcgggct gcggagcccc ggcagcgggc gggactgcat ggtgtgcttt
     2401 gagagtgaag tgacagcagc cctggtgccc tgtggacaca accttttctg catggagtgt
     2461 gcagtacgca tctgcgagag gacggaccca gagtgtcctg tctgccacat caccgccaca
     2521 caagccatcc gaatattttc cggccgcatc ggtaccgaag ccgctagcgc taccggtatg
     2581 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2641 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2701 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     2761 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     2821 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     2881 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     2941 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3001 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3061 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3121 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3181 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3241 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaga gctcgatgag
     3301 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3361 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3421 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     3481 ctctacaaat gtggtactta agagggggag accaaagggc gagacgttaa ggcctcacgt
     3541 gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3721 cgctctcctg ttccgaccct gccgcttacg ggatacctgt ccgcctttct cccttcggga
     3781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4021 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4261 gtcatgccgt ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     4321 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     4381 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     4441 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     4501 ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt
     4561 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     4621 tccatccgag tacgtgctct ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     4681 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     4741 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     4801 cttcccgctt cagtgacaac gtcgagtaca gctgcgcaag gaacgcccgt cgtggccagc
     4861 cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg
     4921 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     4981 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     5041 gcgtgcaatc catcttgttc aatcataata ttattgaagc atttatcagg gtt
//
