LOCUS       sequence-481091-        8803 bp    DNA     circular SYN 18-AUG-2025
DEFINITION  Cell fusion assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, 
            Podbilewicz B
  TITLE     A novel function for the sperm adhesion protein IZUMO1 in cell-cell 
            fusion.
  JOURNAL   J Cell Biol. 2023 Feb 6;222(2):e202207147. doi: 
            10.1083/jcb.202207147. Epub 2022 Nov 17.
  PUBMED    36394541
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-02-6"; volume: "222(2):e202207147. doi"; pages: 
            " 10.1083/jcb.202207147. Epub 2022 Nov 17"
COMMENT     Sequence Label: pCI-AtGCS1-HAP2-V5-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..8803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          645..1661
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1585..1607
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1669..1688
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3910..3951
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3961..3978
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(3995..4569,4570..4572,4573..4581)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4162..4179)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4389..4408
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4588..4965
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4978..4987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4984..4986,4987..4989,4990..5703)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4984..4986,4987..4989,4990..5700)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5029..5050)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5290..5309)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5637..5658
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5795..5814)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5860..5915
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5914..5933)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6306..6322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6306..6322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6319..6341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6330..6346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6354..6360,6361..6378,6379..6384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6399..6420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6479..6674
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6525..6660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6680..6814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6717..6736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6771..6790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6882..6899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7053..7641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7133..7152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7812..8603,8604..8672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8435..8454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8673..8777)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc cccggggggg gggggggggg gggggggggg
      541 gggggggggg ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      601 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      661 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      721 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      781 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      841 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      901 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      961 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1021 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1081 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1141 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1201 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     1261 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1321 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1381 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1441 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1501 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1561 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1621 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1681 ttattgtgct gtctcatcat tttggcaaag aattgctcga gagttcgaag ccttaagtac
     1741 gcgtaggcgc gccttaatta acgtttaaac gctagcggta ccatggtgaa cgcgatttta
     1801 atggcttgta tactcgccgg aattttcgtc ggaatgttca acgaagtcga cggaattcag
     1861 attttatcga aatcaaagct cgaaaagtgt gagaaaacat cagattctgg taatctcaat
     1921 tgttccacta agatcgtctt gaatctcgct gttcccagtg gatccagtgg aggagaggct
     1981 tcgattgtag cggagatcgt ggaggttgaa gataactcga gtagtaatat gcaaaccgta
     2041 cggattccac cggttattac agtgaacaaa tctgcagcat atgctctcta tgatcttact
     2101 tacattaggg atgtacctta taaacctcaa gagtatcatg tcacgactcg aaagtgtgaa
     2161 cccgatgctg gaccggatat tgtacaaatt tgtgagaggt tgagagatga gaagggtaat
     2221 gttttagagc agactcagcc gatttgttgt ccatgtggac cgcaacgaag aatgccttct
     2281 tcttgtggag atatttttga taagatgatt aaagggaagg caaataccgc gcattgcctt
     2341 cgttttccag gtgactggtt ccatgttttc gggattggac aacggtcact aggatttagt
     2401 gtgcgagttg agttgaaaac aggaactaga gtttcagaag tgatcattgg tcctgaaaat
     2461 aggacagcta ctgcaaatga taactttctc aaagttaatc tcataggaga ctttggtggt
     2521 tacacaagta tcccttcttt cgaggacttc tatcttgtaa tcccaagaga ggcggctgaa
     2581 gcgggacaac cagggagctt aggcgcaaat tactcaatgt ggatgctgct tgagagagtg
     2641 agatttacat tggatggctt agaatgtaat aaaatcggtg ttggttatga agctttcaac
     2701 acccaaccaa atttctgctc ttctccatat tggagttgct tgcataatca actatggaac
     2761 ttccgtgagt cagatataaa cagaatcgac cgacaccaac ttcctttata tggacttgaa
     2821 ggaagatttg agaggatcaa tcaacaccca aacgcggggc ctcactcgtt ctcaattgga
     2881 gtcacggaaa cactcaacac aaatctaatg atagaactaa gagctgatga tattgaatac
     2941 gttttccaga ggagtcccgg taagatcata aacatcgcga taccgacatt cgaagcactt
     3001 acacaattcg gagttgctgc agtcataatc aagaatactg gtgaagtaga agcgtcttat
     3061 agcttaacgt ttgattgctc caaaggcgtg gcttttgttg aggaacaatt tttcattata
     3121 aaaccgaaag cggttactac tcggtctttt aagctgtacc cgacgaaaga tcaggctgca
     3181 aagtatatat gcacagctat actcaaggac tctcagttca gcgaagtaga ccgagccgag
     3241 tgtcaattta gtacaacggc taccgtactt gacaatggaa ctcaggttac taatccattt
     3301 caaattccag agactcaacc taaaggtttc tttgattcga tcaggatact atggatgaaa
     3361 atcatcaatg gtttagtcga tttcatcacc ggagatactt gcagaaacaa atgctcgagt
     3421 ttcttcgact tcagttgcca cattcaatac gtttgcttaa gctggatggt tatgtttggt
     3481 cttctcctcg ctctcttccc aattacttgt ttgcttcttt ggctcttgca ccaaaaggga
     3541 ctctttgatc catgctacga ctggtgggaa gatcatttcg atttagacca tcatcggaga
     3601 ttattaccct ccagagcaga cgttgttaac cgccatcatc accatcacaa gcatcgtcat
     3661 caccataatc accatcgtag gacacaccag agacacaaac atcatcatgg tcaagacgac
     3721 gacgtattgc agaagatgat gcttgaacgt gaccacagcg actctcacta ctaccaccaa
     3781 cttcatcgag tccataaaga tagcaagcag aagcagcgta ggcgagccaa gcacgggatc
     3841 gtcctgccac gtgatgtgca cgtggaaaga cagaggaaac aaagactacg tgagagtccg
     3901 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     3961 catcatcacc atcaccatta gcccggggga tccgcccctc tccctccccc ccccctaacg
     4021 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4081 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4141 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4201 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4261 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4321 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4381 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4441 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
     4501 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4561 gatgataata tggccacaac catggttatg ccagagccag cgaagtctgc tcccgccccg
     4621 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
     4681 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
     4741 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
     4801 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
     4861 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
     4921 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc
     4981 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5041 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5101 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5161 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5221 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5281 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5341 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5401 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5461 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5521 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5581 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     5641 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5701 taaagcggcc gcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     5761 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     5821 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     5881 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     5941 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     6001 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     6061 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     6121 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     6181 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     6241 ccctcgacct gcaccgtcga ccagctggtc gacggtgcac cgtcgaccag cttggcgtaa
     6301 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6361 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6421 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     6481 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6601 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6661 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     6721 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6781 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     6841 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7021 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7081 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7141 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7201 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7261 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7321 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7381 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7441 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7501 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7561 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7621 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7681 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7741 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7801 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7861 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7921 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7981 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8041 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8101 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8161 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8221 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8281 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8341 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8401 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8461 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8521 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8581 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8641 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8761 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
