LOCUS       sequence-481096-       14180 bp    DNA     circular SYN 29-JUL-2025
DEFINITION  Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 14180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1G511A-CMyc
FEATURES             Location/Qualifiers
     source          1..14180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    join(64..82,83..105,106..124,125..147,148..166,167..189,
                     190..208,209..233,234..252,253..275,276..294,295..317,
                     318..336)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        369..407
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     404..428
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             516..539
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2505..2534
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(2561..2585)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2635..3846
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3683..3702
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3905..4522,4523..4528,4529..4564,4565..4567,4568..4603,
                     4604..4606,4607..4642,4643..4651)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4704..5277
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4871..4888)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5098..5117
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5293..6087
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5293..6087
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5347..5366)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5957..5976
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6255..6843
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6308..6328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6726..6737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6846..6862)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6847..6863)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7372..7552
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7578..7595)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7584..7808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7854..8282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7941..7960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8151..8172
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8291..8311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8296..8625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8476..8611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8538..8557
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8673..8720
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8739..9113
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9243..9364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9280..9299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9334..9353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9413..9429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9413..9429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9426..9448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9437..9453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9461..9467,9468..9485,9486..9491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9506..9527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9644..9661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9815..10403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9895..9914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10574..11365,11366..11434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11197..11216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11435..11539)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11614..11633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11805..12184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12186..12384
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12402..12582
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12629..12754
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13247..13480
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13665..13709
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14007..14124
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatcctttac
       61 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      121 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      181 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtgtttac cactccctat
      241 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      301 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc
      361 cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
      421 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      481 gatccagcct ccgcggcccc gaactagtca ccatggacta caaggacgac gatgacaaaa
      541 tggcgtccac ggattacagt acctatagcc aagctgcagc gcagcagggc tacagtgctt
      601 acaccgccca gcccactcaa ggatatgcac agaccaccca ggcatatggg caacaaagct
      661 atggaaccta tggacagccc actgatgtca gctataccca ggctcagacc actgcaacct
      721 atgggcagac cgcctatgca acttcttatg gacagcctcc cactggttat actactccaa
      781 ctgcccccca ggcatacagc cagcctgtcc aggggtatgg cactggtgct tatgatacca
      841 ccactgctac agtcaccacc acccaggcct cctatgcagc tcagtctgca tatggcactc
      901 agcctgctta tccagcctat gggcagcagc cagcagccac tgcacctaca agaccgcagg
      961 atggaaacaa gcccactgag actagtcaac ctcaatctag cacagggggt tacaaccagc
     1021 ccagcctagg atatggacag agtaactaca gttatcccca ggtacctggg agctacccca
     1081 tgcagccagt cactgcacct ccatcctacc ctcctaccag ctattcctct acacagccga
     1141 ctagttatga tcagagcagt tactctcagc agaacaccta tgggcaaccg agcagctatg
     1201 gacagcagag tagctatggt caacaaagca gctatgggca gcagcctccc actagttacc
     1261 caccccaaac tggatcctac agccaagctc caagtcaata tagccaacag agcagcagct
     1321 acgggcagca gagttcattc cgacaggacc accccagtag catgggtgtt tatgggcagg
     1381 agtctggagg attttccgga ccaggagaga accggagcat gagtggccct gataaccggg
     1441 gcaggggaag agggggattt gatcgtggag gcatgagcag aggtgggcgg ggaggaggac
     1501 gcggtggaat gggcgctgga gagcgaggtg gcttcaataa gcctggtgga cccatggatg
     1561 aaggaccaga tcttgatcta ggcccacctg tagatccaga tgaagactct gacaacagtg
     1621 caatttatgt acaaggatta aatgacagtg tgactctaga tgatctggca gacttcttta
     1681 agcagtgtgg ggttgttaag atgaacaaga gaactgggca acccatgatc cacatctacc
     1741 tggacaagga aacaggaaag cccaaaggcg atgccacagt gtcctatgaa gacccaccca
     1801 ctgccaaggc tgccgtggaa tggtttgatg ggaaagattt tcaagggagc aaacttaaag
     1861 tctcccttgc tcggaagaag cctccaatga acagtatgcg gggtggtctg ccaccccgtg
     1921 agggcagagg catgccacca ccactccgtg gaggtccagg aggcccagga ggtcctgggg
     1981 gacccatggg tcgcatggga ggccgtggag gagatagagg aggcttccct ccaagaggac
     2041 cccggggttc ccgagggaac ccctctggag caggaaacgt ccagcaccga gctggagact
     2101 ggcagtgtcc caatccgggt tgtggaaacc agaacttcgc ctggagaaca gagtgcaacc
     2161 agtgtaaggc cccaaagcct gaaggcttcc tcccgccacc ctttccgccc ccgggtggtg
     2221 atcgtggcag aggtggccct ggtggcatgc ggggaggaag aggtggcctc atggatcgtg
     2281 gtggtcccgg tggaatgttc agaggtggcc gtggtggaga cagaggtggc ttccgtggtg
     2341 gccggggcat ggaccgaggt ggctttggtg gaggaagacg aggtggccct ggggggcccc
     2401 ctggaccttt gatggaacag atgggaggaa gaagaggagg acgtggagga cctggaaaaa
     2461 tggataaagg cgagcaccgt caggagcgca gagatcggcc ctacgaacaa aaactcatct
     2521 cagaagagga tctgtaggcg gccgcactcg agatatctag acccagcttt cttgtacaaa
     2581 gtggttgata acatctcgag ccataaagat ggttaattaa cccacccaag atctggcctc
     2641 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     2701 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     2761 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     2821 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     2881 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     2941 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     3001 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     3061 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     3121 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     3181 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     3241 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     3301 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     3361 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     3421 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     3481 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     3541 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     3601 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     3661 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     3721 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     3781 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     3841 ttagacgaag cttggtaccg agctcggatc tccaccccgt accggtcctg cagtcgaatt
     3901 caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     3961 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     4021 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     4081 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     4141 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     4201 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     4261 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     4321 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     4381 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     4441 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     4501 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     4561 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     4621 cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc cgcactagag
     4681 gaattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     4741 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     4801 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4861 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4921 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4981 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5041 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5101 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5161 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5221 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     5281 taccggtcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5341 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5401 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5461 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5521 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5581 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5641 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5701 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5761 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5821 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5881 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5941 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6001 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6061 tatcgccttc ttgacgagtt cttctgatgt acaagtaaag cggccgcgac tctagatcat
     6121 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     6181 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt tagtccctcc caattcgata
     6241 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6301 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6361 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6421 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6481 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6541 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6601 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6661 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6721 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6781 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6841 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     6901 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     6961 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7021 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7081 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7141 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7201 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7261 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7321 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7381 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7441 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7501 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7561 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7621 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7681 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7741 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7801 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7861 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7921 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7981 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8041 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8101 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8161 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8221 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8281 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8341 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8401 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8461 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8521 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     8581 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     8641 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     8701 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     8761 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8821 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8881 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     8941 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     9001 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     9061 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     9121 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     9181 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     9241 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9361 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    10501 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    10561 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10621 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10681 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10741 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10801 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10861 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10921 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10981 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11041 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11101 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11161 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11221 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11281 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11341 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11401 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    11461 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    11521 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    11581 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    11641 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    11701 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    11761 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
    11821 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
    11881 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
    11941 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
    12001 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
    12061 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
    12121 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
    12181 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
    12241 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
    12301 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
    12361 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
    12421 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    12481 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    12541 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
    12601 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
    12661 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    12721 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    12781 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    12841 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    12901 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    12961 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    13021 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    13081 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    13141 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    13201 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    13261 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    13321 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    13381 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    13441 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    13501 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    13561 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    13621 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
    13681 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    13741 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    13801 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    13861 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
    13921 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
    13981 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    14041 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    14101 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    14161 ttaattctag acagttatca
//
