LOCUS       sequence-481666-        4988 bp    DNA     circular SYN 01-AUG-2025
DEFINITION  Gateway ORF Entry clone of mouse Tgfbr2 with stop codon (for native 
            or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4988)
  TITLE     Esposito lab plasmids 2025
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8061-E29
FEATURES             Location/Qualifiers
     source          1..4988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2469..2568)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2585..2604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2586..2604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2609..2625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2609..2625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3056..3847
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3969..4078
                     /note="incP origin of transfer"
     rep_origin      4338..4926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4827..4846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgggtcgg gggctgctcc ggggcctgtg
      721 gccgctgcat atcgtcctgt ggacgcgcat cgccagcacg atcccgccgc acgttcccaa
      781 gtcggatgtg gaaatggaag cccagaaaga tgcatccatc cacctaagct gtaataggac
      841 catccatcca ctgaaacatt ttaacagtga tgtcatggcc agcgacaatg gcggtgcggt
      901 caagcttcca cagctgtgca agttttgcga tgtgagactg tccacttgcg acaaccagaa
      961 gtcctgcatg agcaactgca gcatcacggc catctgtgag aagccgcatg aagtctgcgt
     1021 ggccgtgtgg aggaagaacg acaagaacat tactctggag acggtttgcc acgaccccaa
     1081 gctcacctac cacggcttca ctctggaaga tgccgcttct cccaagtgtg tcatgaagga
     1141 aaagaaaagg gcgggcgaga ctttcttcat gtgtgcctgt aacatggaag agtgcaacga
     1201 ttacatcatc ttttcggaag aatacaccac cagcagtccc gacctgttgt tggtcattat
     1261 ccaagtgacg ggtgtcagcc tcctgcctcc gctggggatt gccatagctg tcatcatcat
     1321 cttctactgc taccgtgtcc accggcagca gaagctgagc ccgtcctggg agagcagcaa
     1381 gccccggaaa ctgatggatt tcagtgacaa ttgtgccatc atcctggagg acgaccgctc
     1441 cgacatcagc tccacgtgcg ccaacaacat caaccacaac acggagctgc tgcccatcga
     1501 gctggacacg ctggtgggga agggccgctt cgccgaggtc tacaaggcca agctgaagca
     1561 gaacacctca gagcagtttg agaccgtggc tgtcaagatc ttcccctacg aggagtactc
     1621 ctcgtggaaa acagagaagg acatcttctc cgatatcaac ctgaagcatg agaacatcct
     1681 gcagttcctg acggccgagg agcggaagac agagctgggc aagcagtact ggctgatcac
     1741 ggcgttccac gcgaagggca acctgcagga gtacctcacg aggcatgtca tcagctggga
     1801 ggacctgagg aagctgggca gctccctggc ccggggcatc gctcatctcc acagtgacca
     1861 cactccttgt gggaggccca agatgcccat tgttcacagg gacctcaaga gctctaacat
     1921 cctagtgaag aacgacttga cctgttgcct gtgtgacttc gggctgtcct tgcgcctgga
     1981 ccctactctg tctgtggatg acctggccaa cagcgggcag gtgggaacgg caagatacat
     2041 ggccccggaa gttctagaat ccaggatgaa tctggaaaac gtggagtcgt tcaagcagac
     2101 ggatgtctac tccatggctc tggtactctg ggaaatgacg tcccgctgca atgctgtggg
     2161 agaagtgaag gattacgagc ccccatttgg ttccaaggtg cgggagcacc cctgtgtgga
     2221 gagcatgaaa gacagtgtgc tgagagaccg agggcggccg gaaattccca gcttctggct
     2281 caaccaccag ggcatccaga tcgtgtgtga gactttgacc gagtgctggg accatgaccc
     2341 cgaagcccgt ctcacagcac agtgtgtggc agagcgcttc agtgagctgg agcatccgga
     2401 gagactctct gggaggagct gctcccagga gaagattcca gaagatggct cgctgaacac
     2461 taccaaataa tcaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2521 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2581 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2641 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2701 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2761 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2821 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2881 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2941 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3001 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     3061 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3121 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3181 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3241 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3301 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3361 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3421 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3481 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3541 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3601 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3661 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3721 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3781 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3841 caaataaccc tcgactctag aggatccccg ggtaccgagc tcgaattccg tgataggtgg
     3901 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     3961 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     4021 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     4081 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     4141 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     4201 tgcagcggaa aacggaattc gagctcggta cccggggatc ctctagagtc gagccaccca
     4261 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     4321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4981 cacatgtt
//
