LOCUS       sequence-481753-       11312 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (red) fluorescent reporter of circadian rhythm, based 
            on the murine Nr1d1-gene (REVERBa protein), expression enhanced by 
            SV40 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(4)-pmRevErbA-mScaI3-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2664..2684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2669..2998
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2849..2984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4825..5508
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="DSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTV
                     SVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMA
                     LRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHST
                     GGSGGS"
     primer_bind     complement(4952..4970)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5518..5538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5973..5997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6005..6504
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6040..6063)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6437..6456
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6515..7114
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6515..6534)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7011..7031
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7115..7139)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7157..7745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7210..7230)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7628..7639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7833..8066
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8138..8259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8175..8194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8229..8248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8299..8434
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8454..8473)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8455..8473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8483..8500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8483..8499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8492..8514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8641..9096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8728..8747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8938..8959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9122..9226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9227..9295,9296..10087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9445..9464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10258..10846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10747..10766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11000..11017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11134..11155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11170..11175,11176..11193,11194..11200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11208..11224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11213..11235
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11232..11248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11232..11248
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11267..11287
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11269..11287
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcc
     2101 ccgtgctatg ccagtcctac ccgtgggggc tggggttagg gtgaagaaga gaaaggggtt
     2161 ttatttacac tgcttgtcgc ccaccttgtc accatagcaa aagatgctta agttcttgat
     2221 ttaagggaaa tctctcctcc cagcttgtct ttctccccct agtcaccact aacctcaggg
     2281 tgaggtcagt tctccaatca cgtgaagctc tcacgtttgc taggtttgca gaaagggcct
     2341 tttagctttg atcttccacg gagcagtaaa gcttgccagg ccctccccag gaattcacat
     2401 gcccttgcca tacaaggctt tccaaacacg ccaccctgac tcttcagaaa accccacccc
     2461 agtccacttt cagctcctcc ccgctgccag caagcacttt tgcaaagcag aaaaggaata
     2521 ggcacactcc acctacattg tcagtagact acaaatccca acaatcctgg cggtgcgcag
     2581 gcgcacagat ctcaacgtgc cggctgctgg aaaagtgtgt cactggggca cgaggcgctc
     2641 cctggaatca catggtaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     2941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3001 gctcggtacc tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg
     3061 cagagccctc tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa
     3121 atattactgc tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat
     3181 ttctccatca cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc
     3241 ggagctccag attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca
     3301 ctgcagagac ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc
     3361 acacacacac tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc
     3421 cttctggagg gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg
     3481 attgcgaact gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc
     3541 atcacaacct ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga
     3601 catgacgacc ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg
     3661 ctcacatggc tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt
     3721 ggccgccatc tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca
     3781 agttgccgga gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg
     3841 gttttagggc cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt
     3901 ccttgcgaca gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg
     3961 gtgccacgtg cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc
     4021 ccgccccgcc cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct
     4081 ggcttctttt cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg
     4141 gtaaccattc tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt
     4201 taaggttgcc ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc
     4261 gactcggtgg catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc
     4321 actccaccca tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct
     4381 tctcttctgt gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca
     4441 gagccgctgg ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa
     4501 ttcctaaatc cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga
     4561 gtcttatctg tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc
     4621 tgaacagaga gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat
     4681 ttaggtggtg ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg
     4741 gagtccctct acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc
     4801 acatacttcc caccatcaac gcgtgatagc accgaggcag tgatcaagga gttcatgcgg
     4861 ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     4921 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     4981 ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcatc
     5041 aagcaccccg ccgacatccc cgactactgg aagcagtcct tccccgaggg cttcaagtgg
     5101 gagcgcgtga tgatcttcga ggacggcggc accgtgtccg tgacccagga cacctccctg
     5161 gaggacggca ccctgatcta caaggtgaag ctccgcggcg gcaacttccc tcctgacggc
     5221 cccgtaatgc agaagaggac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag
     5281 gacgtcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac
     5341 ctggcggact tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgccttc
     5401 aacatcgacc gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag
     5461 tacgaacgct ccgtggcccg ccactccacc ggcggctccg gtggctcctc tagacctcca
     5521 aaaaagaaga gaaaggtaga agaccccttg tacaagagcc atggcttccc gccggctgtg
     5581 gctgctcagg atgatggcac gctgcccatg tcctgtgccc aggagagcgg gatggaccgt
     5641 caccctgcag cctgtgctcc tgctaggatc aatgtgtgac tcgagcccgc ccggccggcc
     5701 ttctgccgct gcccccttgt acagaatcga actctgcact tctctctcct ttacgagacg
     5761 aaaaggaaaa gcaaaccaga atcttattta tattgttata aaatattcca agatgagcct
     5821 ctggccccct gagccttctt gtaaataact cttcccccac ccccaccgcc atgctcccat
     5881 cctcccctat ttaaaccact cttgctctct ccaccctcct ctggcccctc gatttgttct
     5941 gttcctgtct caaatccaat agttcacagc tgacaagttt gtacaaaaaa gcaggctttc
     6001 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     6061 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     6121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     6181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     6241 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     6301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     6361 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     6421 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     6481 tcttcctcat ctccgggcct ttcgacctgc caccatgacc gagtacaagc ccacggtgcg
     6541 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6601 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6661 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6721 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6781 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6841 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6901 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6961 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7021 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7081 cacctggtgc atgacccgca agcccggtgc ctgaacccag ctttcttgta caaagtggtg
     7141 ataatcgaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7201 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7261 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7321 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7381 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7441 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7501 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7561 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7621 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7681 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7741 cccgcatcgg gaattcccgc ggttcgcttt aagaccaatg acttacaagg cagctgtaga
     7801 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7861 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7921 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7981 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8041 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     8101 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     8161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     8281 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8341 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8401 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     8461 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     8641 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8701 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8761 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8821 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8881 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     9001 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9061 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     9121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    10021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    10081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10321 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10381 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10441 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10501 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10561 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10621 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag
    10681 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10741 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10801 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10861 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10921 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10981 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    11041 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    11101 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    11161 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    11221 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
    11281 ctaaagggaa caaaagctgg agctgcaagc tt
//
