LOCUS       sequence-481756-       11201 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (yellow) fluorescent reporter of circadian rhythm, 
            based on the murine Nr1d1-gene (REVERBa protein), expression 
            enhanced by SV40 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(2)-pmRevErbA-Venus-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1963..1983)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1968..2297
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2148..2283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4684..4686,4687..5397)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4726..4747)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4987..5006)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5334..5355
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5407..5427
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5862..5886
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5894..6393
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5929..5952)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6326..6345
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6404..7003
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6404..6423)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6900..6920
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7004..7028)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7046..7634
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7099..7119)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7517..7528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7722..7955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8027..8148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8064..8083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8118..8137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8188..8323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8343..8362)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8344..8362)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8372..8389)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8372..8388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8381..8403)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8530..8985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8617..8636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8827..8848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9011..9115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9116..9184,9185..9976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9334..9353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10147..10735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10636..10655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10889..10906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11023..11044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11059..11064,11065..11082,11083..11089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11097..11113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11102..11124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11121..11137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11121..11137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11156..11176
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11158..11176
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgct gtggaatgtg tgtcagttag
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2101 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2221 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2281 gcctaggctt ttgcaaaaag ctcgatatcc ccgtgctatg ccagtcctac ccgtgggggc
     2341 tggggttagg gtgaagaaga gaaaggggtt ttatttacac tgcttgtcgc ccaccttgtc
     2401 accatagcaa aagatgctta agttcttgat ttaagggaaa tctctcctcc cagcttgtct
     2461 ttctccccct agtcaccact aacctcaggg tgaggtcagt tctccaatca cgtgaagctc
     2521 tcacgtttgc taggtttgca gaaagggcct tttagctttg atcttccacg gagcagtaaa
     2581 gcttgccagg ccctccccag gaattcacat gcccttgcca tacaaggctt tccaaacacg
     2641 ccaccctgac tcttcagaaa accccacccc agtccacttt cagctcctcc ccgctgccag
     2701 caagcacttt tgcaaagcag aaaaggaata ggcacactcc acctacattg tcagtagact
     2761 acaaatccca acaatcctgg cggtgcgcag gcgcacagat ctcaacgtgc cggctgctgg
     2821 aaaagtgtgt cactggggca cgaggcgctc cctggaatca catggtacct gctccagtgc
     2881 cgcgtgcggc ccgggaaccc tgggctgctg gcgcctgcgc agagccctct gtcccaggga
     2941 aaggctcggg caaaaggcgg ttgagattgg cagagtgaaa tattactgct gaggaaacgt
     3001 agcagggcac acgtctctct ttgcgtcctg gtgcctcatt tctccatcac ctacttactt
     3061 cctggttgca gcttctcttc ctttgggact tttgcacccg gagctccaga ttcattaccc
     3121 tgcttcactg cggagccttc aggagtggca tccggtgcac tgcagagacc cgactctcct
     3181 tgctacctta tagccagaac tgctgcaggc tgattcttca cacacacact ctctgctctt
     3241 cccatgcaaa tcagatctca ggtgcctcag cgtcctaccc ttctggaggg ctgcagtata
     3301 gccaccccaa gaccttactg ctcagtgcct ggaatcctga ttgcgaactg cggggctcac
     3361 tcgtctccct cagccattgc ccagggggcg agagaggcca tcacaacctc cagtttgtgt
     3421 caaggtccag tttgaatgac cgctttcagc tggtgaagac atgacgaccc tggactccaa
     3481 taacaacaca ggtactgaga ttcttatctt tgctcagggc tcacatggct gcagagccgg
     3541 gcccacgtgc tgcatttgtt ttcattcagt agaagccgtg gccgccatct cattggtcat
     3601 tgcctcccgg cctcgttaca taatgagctc cgcccctcaa gttgccggag caacgcttgt
     3661 cgccttctct cctcccctca gttgtcccgg cccagcttgg ttttagggcc agcctacgac
     3721 ccaggacgcc cgggaggggc ggggcgaggg cggggttttc cttgcgacag aagggaagcg
     3781 gagaaggcgg tgcttcgagt tccggggttc ctcgttgggg tgccacgtgc gaggggcaca
     3841 cgtggagtgg ggacgtgagg ccagctgagg gccaggctcc cgccccgccc ttctccgcct
     3901 agatctgggt gaagctgggc tgactcaccg cccccgcctg gcttcttttc ctctcagaaa
     3961 ataacccatc ctccaaccca gccgtgaaac catagccggg taaccattct gtgacttatt
     4021 ttacaggcag ggttcaccct gaccaacctt gagctatttt aaggttgccc tgcctggttt
     4081 agtgtgtgtc agtgggtgag caagacatgt gcttctgtcg actcggtggc atggcatgtg
     4141 tcacctcaca cacgtgtctg aggatcctcc cctttcccca ctccacccat ggccggccag
     4201 gctaagtact tggcagaggt gtggcagaca agtggccctt ctcttctgtg cataccacag
     4261 gagccgaatc acctggcccg caatgtaacc agagggacag agccgctggc tgagtggcta
     4321 tagagctaaa gttaccaccg ggctgagaag gaaggggaat tcctaaatcc ctagaatgga
     4381 tactttgttt ctccctttca tctcctgcaa ctcatctgag tcttatctgt cacatttcta
     4441 agtctcttgt gactcatctc tcagaggcca gagccaggct gaacagagag tccttccctg
     4501 cagtactcta tgagaatatg tctttgggtg ttctcttatt taggtggtgt tatcacctac
     4561 attggctcta gtggctcctc cccgagccgg accagcccgg agtccctcta cagtgacagc
     4621 tccaatggca gcttccagtc cctgactcaa ggttgtccca catacttccc accatcaacg
     4681 cgtgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4741 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4801 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4861 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     4921 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4981 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5041 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5101 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     5161 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     5221 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5281 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5341 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaatct
     5401 agacctccaa aaaagaagag aaaggtagaa gaccccttgt acaagagcca tggcttcccg
     5461 ccggctgtgg ctgctcagga tgatggcacg ctgcccatgt cctgtgccca ggagagcggg
     5521 atggaccgtc accctgcagc ctgtgctcct gctaggatca atgtgtgact cgagcccgcc
     5581 cggccggcct tctgccgctg cccccttgta cagaatcgaa ctctgcactt ctctctcctt
     5641 tacgagacga aaaggaaaag caaaccagaa tcttatttat attgttataa aatattccaa
     5701 gatgagcctc tggccccctg agccttcttg taaataactc ttcccccacc cccaccgcca
     5761 tgctcccatc ctcccctatt taaaccactc ttgctctctc caccctcctc tggcccctcg
     5821 atttgttctg ttcctgtctc aaatccaata gttcacagct gacaagtttg tacaaaaaag
     5881 caggctttct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5941 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     6001 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     6061 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     6121 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     6181 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     6241 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     6301 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     6361 ctgttctcct cttcctcatc tccgggcctt tcgacctgcc accatgaccg agtacaagcc
     6421 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6481 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6541 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6601 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6661 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6721 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6781 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6841 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6901 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6961 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacccagc tttcttgtac
     7021 aaagtggtga taatcgaatt ccgataatca acctctggat tacaaaattt gtgaaagatt
     7081 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7141 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7201 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7261 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7321 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7381 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7441 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7501 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7561 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7621 ggccgcctcc ccgcatcggg aattcccgcg gttcgcttta agaccaatga cttacaaggc
     7681 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7741 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7801 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7861 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7921 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7981 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     8041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     8161 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8221 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8281 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     8341 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     8401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     8461 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8521 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     8581 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     8641 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8701 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8761 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8821 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8881 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8941 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     9001 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9061 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9121 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9181 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9241 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9301 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     9361 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9421 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9481 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9541 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9601 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9661 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9721 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9781 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9841 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9901 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9961 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    10021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    10081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10261 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    10321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    10561 cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    10621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    10681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    10741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    10801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    10921 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    10981 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    11041 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    11101 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
    11161 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
