LOCUS       sequence-481757-       11143 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (red) fluorescent reporter of circadian rhythm, based 
            on the murine Nr1d1-gene (REVERBa protein), expression enhanced by 
            SV40 promoter fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(3)-pmRevErbA-mScaI3-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2664..2684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             4656..5339
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="DSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTV
                     SVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMA
                     LRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHST
                     GGSGGS"
     primer_bind     complement(4783..4801)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5349..5369
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5804..5828
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5836..6335
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5871..5894)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6268..6287
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6346..6945
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6346..6365)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6842..6862
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6946..6970)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6988..7576
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7041..7061)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7459..7470)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7664..7897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7969..8090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8006..8025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8060..8079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8130..8265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8285..8304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8286..8304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8314..8331)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8314..8330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8323..8345)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8472..8927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8559..8578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8769..8790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8953..9057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9058..9126,9127..9918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9276..9295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10089..10677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10578..10597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10831..10848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10965..10986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11001..11006,11007..11024,11025..11031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11039..11055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11044..11066
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11063..11079
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11063..11079
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11098..11118
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11100..11118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcc
     2101 ccgtgctatg ccagtcctac ccgtgggggc tggggttagg gtgaagaaga gaaaggggtt
     2161 ttatttacac tgcttgtcgc ccaccttgtc accatagcaa aagatgctta agttcttgat
     2221 ttaagggaaa tctctcctcc cagcttgtct ttctccccct agtcaccact aacctcaggg
     2281 tgaggtcagt tctccaatca cgtgaagctc tcacgtttgc taggtttgca gaaagggcct
     2341 tttagctttg atcttccacg gagcagtaaa gcttgccagg ccctccccag gaattcacat
     2401 gcccttgcca tacaaggctt tccaaacacg ccaccctgac tcttcagaaa accccacccc
     2461 agtccacttt cagctcctcc ccgctgccag caagcacttt tgcaaagcag aaaaggaata
     2521 ggcacactcc acctacattg tcagtagact acaaatccca acaatcctgg cggtgcgcag
     2581 gcgcacagat ctcaacgtgc cggctgctgg aaaagtgtgt cactggggca cgaggcgctc
     2641 cctggaatca catggtaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccatagtc ccgccggtac ctgctccagt gccgcgtgcg gcccgggaac cctgggctgc
     2881 tggcgcctgc gcagagccct ctgtcccagg gaaaggctcg ggcaaaaggc ggttgagatt
     2941 ggcagagtga aatattactg ctgaggaaac gtagcagggc acacgtctct ctttgcgtcc
     3001 tggtgcctca tttctccatc acctacttac ttcctggttg cagcttctct tcctttggga
     3061 cttttgcacc cggagctcca gattcattac cctgcttcac tgcggagcct tcaggagtgg
     3121 catccggtgc actgcagaga cccgactctc cttgctacct tatagccaga actgctgcag
     3181 gctgattctt cacacacaca ctctctgctc ttcccatgca aatcagatct caggtgcctc
     3241 agcgtcctac ccttctggag ggctgcagta tagccacccc aagaccttac tgctcagtgc
     3301 ctggaatcct gattgcgaac tgcggggctc actcgtctcc ctcagccatt gcccaggggg
     3361 cgagagaggc catcacaacc tccagtttgt gtcaaggtcc agtttgaatg accgctttca
     3421 gctggtgaag acatgacgac cctggactcc aataacaaca caggtactga gattcttatc
     3481 tttgctcagg gctcacatgg ctgcagagcc gggcccacgt gctgcatttg ttttcattca
     3541 gtagaagccg tggccgccat ctcattggtc attgcctccc ggcctcgtta cataatgagc
     3601 tccgcccctc aagttgccgg agcaacgctt gtcgccttct ctcctcccct cagttgtccc
     3661 ggcccagctt ggttttaggg ccagcctacg acccaggacg cccgggaggg gcggggcgag
     3721 ggcggggttt tccttgcgac agaagggaag cggagaaggc ggtgcttcga gttccggggt
     3781 tcctcgttgg ggtgccacgt gcgaggggca cacgtggagt ggggacgtga ggccagctga
     3841 gggccaggct cccgccccgc ccttctccgc ctagatctgg gtgaagctgg gctgactcac
     3901 cgcccccgcc tggcttcttt tcctctcaga aaataaccca tcctccaacc cagccgtgaa
     3961 accatagccg ggtaaccatt ctgtgactta ttttacaggc agggttcacc ctgaccaacc
     4021 ttgagctatt ttaaggttgc cctgcctggt ttagtgtgtg tcagtgggtg agcaagacat
     4081 gtgcttctgt cgactcggtg gcatggcatg tgtcacctca cacacgtgtc tgaggatcct
     4141 cccctttccc cactccaccc atggccggcc aggctaagta cttggcagag gtgtggcaga
     4201 caagtggccc ttctcttctg tgcataccac aggagccgaa tcacctggcc cgcaatgtaa
     4261 ccagagggac agagccgctg gctgagtggc tatagagcta aagttaccac cgggctgaga
     4321 aggaagggga attcctaaat ccctagaatg gatactttgt ttctcccttt catctcctgc
     4381 aactcatctg agtcttatct gtcacatttc taagtctctt gtgactcatc tctcagaggc
     4441 cagagccagg ctgaacagag agtccttccc tgcagtactc tatgagaata tgtctttggg
     4501 tgttctctta tttaggtggt gttatcacct acattggctc tagtggctcc tccccgagcc
     4561 ggaccagccc ggagtccctc tacagtgaca gctccaatgg cagcttccag tccctgactc
     4621 aaggttgtcc cacatacttc ccaccatcaa cgcgtgatag caccgaggca gtgatcaagg
     4681 agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg
     4741 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     4801 agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca
     4861 gggccttcat caagcacccc gccgacatcc ccgactactg gaagcagtcc ttccccgagg
     4921 gcttcaagtg ggagcgcgtg atgatcttcg aggacggcgg caccgtgtcc gtgacccagg
     4981 acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc ggcaacttcc
     5041 ctcctgacgg ccccgtaatg cagaagagga caatgggctg ggaagcatcc accgagcggt
     5101 tgtaccccga ggacgtcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg
     5161 gcggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc
     5221 ccggcgcctt caacatcgac cgcaagttgg acatcacctc ccacaacgag gactacaccg
     5281 tggtggaaca gtacgaacgc tccgtggccc gccactccac cggcggctcc ggtggctcct
     5341 ctagacctcc aaaaaagaag agaaaggtag aagacccctt gtacaagagc catggcttcc
     5401 cgccggctgt ggctgctcag gatgatggca cgctgcccat gtcctgtgcc caggagagcg
     5461 ggatggaccg tcaccctgca gcctgtgctc ctgctaggat caatgtgtga ctcgagcccg
     5521 cccggccggc cttctgccgc tgcccccttg tacagaatcg aactctgcac ttctctctcc
     5581 tttacgagac gaaaaggaaa agcaaaccag aatcttattt atattgttat aaaatattcc
     5641 aagatgagcc tctggccccc tgagccttct tgtaaataac tcttccccca cccccaccgc
     5701 catgctccca tcctccccta tttaaaccac tcttgctctc tccaccctcc tctggcccct
     5761 cgatttgttc tgttcctgtc tcaaatccaa tagttcacag ctgacaagtt tgtacaaaaa
     5821 agcaggcttt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     5881 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     5941 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     6001 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     6061 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     6121 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     6181 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     6241 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     6301 cgctgttctc ctcttcctca tctccgggcc tttcgacctg ccaccatgac cgagtacaag
     6361 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6421 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6481 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6541 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6601 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6661 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6721 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6781 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6841 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6901 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaccca gctttcttgt
     6961 acaaagtggt gataatcgaa ttccgataat caacctctgg attacaaaat ttgtgaaaga
     7021 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7081 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7141 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7201 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7261 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7321 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7381 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7441 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7501 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7561 tgggccgcct ccccgcatcg ggaattcccg cggttcgctt taagaccaat gacttacaag
     7621 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7681 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7741 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7801 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7861 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7921 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8101 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8161 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8221 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     8281 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     8341 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8401 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8461 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8521 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8581 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8641 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8701 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8761 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8821 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8881 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     8941 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9001 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9061 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9121 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9181 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9241 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9301 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9361 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9421 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9481 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9541 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9601 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9661 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9721 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9781 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9841 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9901 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9961 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10201 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10501 cccgaagaga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10861 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    10921 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    10981 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11041 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
    11101 attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
