LOCUS       sequence-481759-       11186 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (blue) fluorescent reporter of circadian rhythm, based 
            on the murine Nr1d1-gene (REVERBa protein), expression enhanced by 
            synthtic JeT promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5392..5412
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5847..5871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5879..6378
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5914..5937)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6311..6330
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6389..6988
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6389..6408)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6885..6905
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6989..7013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7031..7619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7084..7104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7502..7513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7707..7940
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8012..8133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8049..8068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8103..8122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8173..8308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8328..8347)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8329..8347)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8357..8374)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8357..8373)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8366..8388)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8515..8970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8602..8621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8812..8833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8996..9100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9101..9169,9170..9961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9319..9338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10132..10720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10621..10640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10874..10891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11008..11029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11044..11049,11050..11067,11068..11074)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11082..11098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11087..11109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11106..11122
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11106..11122
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11141..11161
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11143..11161
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcg
     2101 aattcgggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta
     2161 tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     2221 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatccctgtg
     2281 atcgtcactt gacagatatc cccgtgctat gccagtccta cccgtggggg ctggggttag
     2341 ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg cccaccttgt caccatagca
     2401 aaagatgctt aagttcttga tttaagggaa atctctcctc ccagcttgtc tttctccccc
     2461 tagtcaccac taacctcagg gtgaggtcag ttctccaatc acgtgaagct ctcacgtttg
     2521 ctaggtttgc agaaagggcc ttttagcttt gatcttccac ggagcagtaa agcttgccag
     2581 gccctcccca ggaattcaca tgcccttgcc atacaaggct ttccaaacac gccaccctga
     2641 ctcttcagaa aaccccaccc cagtccactt tcagctcctc cccgctgcca gcaagcactt
     2701 ttgcaaagca gaaaaggaat aggcacactc cacctacatt gtcagtagac tacaaatccc
     2761 aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg ccggctgctg gaaaagtgtg
     2821 tcactggggc acgaggcgct ccctggaatc acatggtacc tgctccagtg ccgcgtgcgg
     2881 cccgggaacc ctgggctgct ggcgcctgcg cagagccctc tgtcccaggg aaaggctcgg
     2941 gcaaaaggcg gttgagattg gcagagtgaa atattactgc tgaggaaacg tagcagggca
     3001 cacgtctctc tttgcgtcct ggtgcctcat ttctccatca cctacttact tcctggttgc
     3061 agcttctctt cctttgggac ttttgcaccc ggagctccag attcattacc ctgcttcact
     3121 gcggagcctt caggagtggc atccggtgca ctgcagagac ccgactctcc ttgctacctt
     3181 atagccagaa ctgctgcagg ctgattcttc acacacacac tctctgctct tcccatgcaa
     3241 atcagatctc aggtgcctca gcgtcctacc cttctggagg gctgcagtat agccacccca
     3301 agaccttact gctcagtgcc tggaatcctg attgcgaact gcggggctca ctcgtctccc
     3361 tcagccattg cccagggggc gagagaggcc atcacaacct ccagtttgtg tcaaggtcca
     3421 gtttgaatga ccgctttcag ctggtgaaga catgacgacc ctggactcca ataacaacac
     3481 aggtactgag attcttatct ttgctcaggg ctcacatggc tgcagagccg ggcccacgtg
     3541 ctgcatttgt tttcattcag tagaagccgt ggccgccatc tcattggtca ttgcctcccg
     3601 gcctcgttac ataatgagct ccgcccctca agttgccgga gcaacgcttg tcgccttctc
     3661 tcctcccctc agttgtcccg gcccagcttg gttttagggc cagcctacga cccaggacgc
     3721 ccgggagggg cggggcgagg gcggggtttt ccttgcgaca gaagggaagc ggagaaggcg
     3781 gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg cgaggggcac acgtggagtg
     3841 gggacgtgag gccagctgag ggccaggctc ccgccccgcc cttctccgcc tagatctggg
     3901 tgaagctggg ctgactcacc gcccccgcct ggcttctttt cctctcagaa aataacccat
     3961 cctccaaccc agccgtgaaa ccatagccgg gtaaccattc tgtgacttat tttacaggca
     4021 gggttcaccc tgaccaacct tgagctattt taaggttgcc ctgcctggtt tagtgtgtgt
     4081 cagtgggtga gcaagacatg tgcttctgtc gactcggtgg catggcatgt gtcacctcac
     4141 acacgtgtct gaggatcctc ccctttcccc actccaccca tggccggcca ggctaagtac
     4201 ttggcagagg tgtggcagac aagtggccct tctcttctgt gcataccaca ggagccgaat
     4261 cacctggccc gcaatgtaac cagagggaca gagccgctgg ctgagtggct atagagctaa
     4321 agttaccacc gggctgagaa ggaaggggaa ttcctaaatc cctagaatgg atactttgtt
     4381 tctccctttc atctcctgca actcatctga gtcttatctg tcacatttct aagtctcttg
     4441 tgactcatct ctcagaggcc agagccaggc tgaacagaga gtccttccct gcagtactct
     4501 atgagaatat gtctttgggt gttctcttat ttaggtggtg ttatcaccta cattggctct
     4561 agtggctcct ccccgagccg gaccagcccg gagtccctct acagtgacag ctccaatggc
     4621 agcttccagt ccctgactca aggttgtccc acatacttcc caccatcaac gcgtgtgtct
     4681 aagggcgaag agctgatcga ggaaaatatg cgtatgaagg tggtcatgga aggttcggtc
     4741 aacggccacc aattcaaatg cacaggtgaa ggagaaggca gaccgtacga gggagtgcaa
     4801 accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga cattcttgcc
     4861 acgtcgttcc tgttcggcag caagaccttt atcaagtacc cggccgacat ccctgatttc
     4921 ttcgaacagt cctttcctga gggctttact tgggaaagag ttacgagata cgaagatggt
     4981 ggagtagtca ccgtcacgca ggacaccagc cttgaggatg gtgggctcgt ctacaacgtc
     5041 aaggtcagag gggtaaactt tcactccaag ggtcccgtga tgcagaagaa gaccgagggt
     5101 tgggagcctt tcacagagat gatgtatcca gcagatggtg gtctgagagg atataccgac
     5161 atcgcactga aagttgatgg tggtggccat ctgcacgcca acatcgtgac aacttacagg
     5221 tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc atgccgttga ttaccgcctg
     5281 gaaaggatcg aagagagtga caatgaaacc tacgtagtgc taagagaagt ggcagttgcc
     5341 aaatacagca accttggtgg tggcatggac gagctgttca agtctagacc tccaaaaaag
     5401 aagagaaagg tagaagaccc cttgtacaag agccatggct tcccgccggc tgtggctgct
     5461 caggatgatg gcacgctgcc catgtcctgt gcccaggaga gcgggatgga ccgtcaccct
     5521 gcagcctgtg ctcctgctag gatcaatgtg tgactcgagc ccgcccggcc ggccttctgc
     5581 cgctgccccc ttgtacagaa tcgaactctg cacttctctc tcctttacga gacgaaaagg
     5641 aaaagcaaac cagaatctta tttatattgt tataaaatat tccaagatga gcctctggcc
     5701 ccctgagcct tcttgtaaat aactcttccc ccacccccac cgccatgctc ccatcctccc
     5761 ctatttaaac cactcttgct ctctccaccc tcctctggcc cctcgatttg ttctgttcct
     5821 gtctcaaatc caatagttca cagctgacaa gtttgtacaa aaaagcaggc tttctaccgg
     5881 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     5941 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     6001 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     6061 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     6121 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     6181 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     6241 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     6301 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     6361 tcatctccgg gcctttcgac ctgccaccat gaccgagtac aagcccacgg tgcgcctcgc
     6421 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6481 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6541 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6601 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6661 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6721 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6781 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6841 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6901 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6961 gtgcatgacc cgcaagcccg gtgcctgaac ccagctttct tgtacaaagt ggtgataatc
     7021 gaattccgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7081 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7141 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7201 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7261 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7321 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7381 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7441 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7501 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7561 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7621 tcgggaattc ccgcggttcg ctttaagacc aatgacttac aaggcagctg tagatcttag
     7681 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7741 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7801 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7861 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7921 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7981 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8041 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8101 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8161 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8221 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8281 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8341 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8401 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8461 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8581 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8701 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8761 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8821 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8881 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8941 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     9001 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9061 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9121 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9181 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9241 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9301 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9361 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9421 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9481 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9541 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9601 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9661 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9721 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9781 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9841 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9901 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9961 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10021 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10081 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10141 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10201 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10261 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10321 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10381 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10441 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10501 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag agagaaaggc
    10561 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10621 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10681 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10741 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10801 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10861 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10921 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10981 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    11041 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    11101 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    11161 ggaacaaaag ctggagctgc aagctt
//
