LOCUS       sequence-481763-       11162 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (red) fluorescent reporter of circadian rhythm, based 
            on the murine Nr1d1-gene (REVERBa protein), expression enhanced by 
            synthtic JeT promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-JeT-pmRevErbA-mScaI3-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4675..5358
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="DSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTV
                     SVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMA
                     LRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHST
                     GGSGGS"
     primer_bind     complement(4802..4820)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5368..5388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5823..5847
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5855..6354
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5890..5913)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6287..6306
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6365..6964
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6365..6384)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6861..6881
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6965..6989)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7007..7595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7060..7080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7478..7489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7683..7916
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7988..8109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8025..8044)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8079..8098
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8149..8284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8304..8323)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8305..8323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8333..8350)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8333..8349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8342..8364)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8491..8946
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8578..8597)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8788..8809
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8972..9076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9077..9145,9146..9937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9295..9314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10108..10696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10597..10616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10850..10867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10984..11005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11020..11025,11026..11043,11044..11050)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11058..11074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11063..11085
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11082..11098
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11082..11098
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11117..11137
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11119..11137
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcg
     2101 aattcgggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta
     2161 tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     2221 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatccctgtg
     2281 atcgtcactt gacagatatc cccgtgctat gccagtccta cccgtggggg ctggggttag
     2341 ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg cccaccttgt caccatagca
     2401 aaagatgctt aagttcttga tttaagggaa atctctcctc ccagcttgtc tttctccccc
     2461 tagtcaccac taacctcagg gtgaggtcag ttctccaatc acgtgaagct ctcacgtttg
     2521 ctaggtttgc agaaagggcc ttttagcttt gatcttccac ggagcagtaa agcttgccag
     2581 gccctcccca ggaattcaca tgcccttgcc atacaaggct ttccaaacac gccaccctga
     2641 ctcttcagaa aaccccaccc cagtccactt tcagctcctc cccgctgcca gcaagcactt
     2701 ttgcaaagca gaaaaggaat aggcacactc cacctacatt gtcagtagac tacaaatccc
     2761 aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg ccggctgctg gaaaagtgtg
     2821 tcactggggc acgaggcgct ccctggaatc acatggtacc tgctccagtg ccgcgtgcgg
     2881 cccgggaacc ctgggctgct ggcgcctgcg cagagccctc tgtcccaggg aaaggctcgg
     2941 gcaaaaggcg gttgagattg gcagagtgaa atattactgc tgaggaaacg tagcagggca
     3001 cacgtctctc tttgcgtcct ggtgcctcat ttctccatca cctacttact tcctggttgc
     3061 agcttctctt cctttgggac ttttgcaccc ggagctccag attcattacc ctgcttcact
     3121 gcggagcctt caggagtggc atccggtgca ctgcagagac ccgactctcc ttgctacctt
     3181 atagccagaa ctgctgcagg ctgattcttc acacacacac tctctgctct tcccatgcaa
     3241 atcagatctc aggtgcctca gcgtcctacc cttctggagg gctgcagtat agccacccca
     3301 agaccttact gctcagtgcc tggaatcctg attgcgaact gcggggctca ctcgtctccc
     3361 tcagccattg cccagggggc gagagaggcc atcacaacct ccagtttgtg tcaaggtcca
     3421 gtttgaatga ccgctttcag ctggtgaaga catgacgacc ctggactcca ataacaacac
     3481 aggtactgag attcttatct ttgctcaggg ctcacatggc tgcagagccg ggcccacgtg
     3541 ctgcatttgt tttcattcag tagaagccgt ggccgccatc tcattggtca ttgcctcccg
     3601 gcctcgttac ataatgagct ccgcccctca agttgccgga gcaacgcttg tcgccttctc
     3661 tcctcccctc agttgtcccg gcccagcttg gttttagggc cagcctacga cccaggacgc
     3721 ccgggagggg cggggcgagg gcggggtttt ccttgcgaca gaagggaagc ggagaaggcg
     3781 gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg cgaggggcac acgtggagtg
     3841 gggacgtgag gccagctgag ggccaggctc ccgccccgcc cttctccgcc tagatctggg
     3901 tgaagctggg ctgactcacc gcccccgcct ggcttctttt cctctcagaa aataacccat
     3961 cctccaaccc agccgtgaaa ccatagccgg gtaaccattc tgtgacttat tttacaggca
     4021 gggttcaccc tgaccaacct tgagctattt taaggttgcc ctgcctggtt tagtgtgtgt
     4081 cagtgggtga gcaagacatg tgcttctgtc gactcggtgg catggcatgt gtcacctcac
     4141 acacgtgtct gaggatcctc ccctttcccc actccaccca tggccggcca ggctaagtac
     4201 ttggcagagg tgtggcagac aagtggccct tctcttctgt gcataccaca ggagccgaat
     4261 cacctggccc gcaatgtaac cagagggaca gagccgctgg ctgagtggct atagagctaa
     4321 agttaccacc gggctgagaa ggaaggggaa ttcctaaatc cctagaatgg atactttgtt
     4381 tctccctttc atctcctgca actcatctga gtcttatctg tcacatttct aagtctcttg
     4441 tgactcatct ctcagaggcc agagccaggc tgaacagaga gtccttccct gcagtactct
     4501 atgagaatat gtctttgggt gttctcttat ttaggtggtg ttatcaccta cattggctct
     4561 agtggctcct ccccgagccg gaccagcccg gagtccctct acagtgacag ctccaatggc
     4621 agcttccagt ccctgactca aggttgtccc acatacttcc caccatcaac gcgtgatagc
     4681 accgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     4741 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     4801 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     4861 cagttcatgt acggctccag ggccttcatc aagcaccccg ccgacatccc cgactactgg
     4921 aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgatcttcga ggacggcggc
     4981 accgtgtccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     5041 ctccgcggcg gcaacttccc tcctgacggc cccgtaatgc agaagaggac aatgggctgg
     5101 gaagcatcca ccgagcggtt gtaccccgag gacgtcgtgc tgaagggcga cattaagatg
     5161 gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac ctacaaggcc
     5221 aagaagcccg tgcagatgcc cggcgccttc aacatcgacc gcaagttgga catcacctcc
     5281 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgtggcccg ccactccacc
     5341 ggcggctccg gtggctcctc tagacctcca aaaaagaaga gaaaggtaga agaccccttg
     5401 tacaagagcc atggcttccc gccggctgtg gctgctcagg atgatggcac gctgcccatg
     5461 tcctgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgctcc tgctaggatc
     5521 aatgtgtgac tcgagcccgc ccggccggcc ttctgccgct gcccccttgt acagaatcga
     5581 actctgcact tctctctcct ttacgagacg aaaaggaaaa gcaaaccaga atcttattta
     5641 tattgttata aaatattcca agatgagcct ctggccccct gagccttctt gtaaataact
     5701 cttcccccac ccccaccgcc atgctcccat cctcccctat ttaaaccact cttgctctct
     5761 ccaccctcct ctggcccctc gatttgttct gttcctgtct caaatccaat agttcacagc
     5821 tgacaagttt gtacaaaaaa gcaggctttc taccgggtag gggaggcgct tttcccaagg
     5881 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     5941 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6001 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6061 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6121 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6181 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6241 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6301 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6361 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6421 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6481 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6541 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6601 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6661 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6721 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6781 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6841 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6901 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6961 ctgaacccag ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga
     7021 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7081 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7141 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7201 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7261 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7321 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7381 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7441 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7501 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7561 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgcttt
     7621 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7681 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     7741 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7801 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7861 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7921 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7981 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8101 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8221 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8281 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     8341 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8401 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8461 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     8521 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8581 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8641 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8701 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8761 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8821 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8881 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     8941 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9001 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9061 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9121 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9181 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9241 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9301 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9361 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9421 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9481 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9541 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9601 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9661 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9721 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9781 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9841 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9901 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9961 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10021 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10501 atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag
    10561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10801 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    10861 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    10921 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    10981 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    11041 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    11101 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
    11161 tt
//
