LOCUS       sequence-481764-       11002 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (red) fluorescent reporter of circadian rhythm, based 
            on the murine Nr1d1-gene (REVERBa protein), expression enhanced by 
            SV40 promoter fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1963..1983)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             4515..5198
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="DSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGTV
                     SVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKMA
                     LRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHST
                     GGSGGS"
     primer_bind     complement(4642..4660)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5208..5228
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5663..5687
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5695..6194
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5730..5753)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6127..6146
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6205..6804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6205..6224)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6701..6721
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6805..6829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6847..7435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6900..6920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7318..7329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7523..7756
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7828..7949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7865..7884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7919..7938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7989..8124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8144..8163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8145..8163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8173..8190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8173..8189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8182..8204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8331..8786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8418..8437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8628..8649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8812..8916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8917..8985,8986..9777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9135..9154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9948..10536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10437..10456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10690..10707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10824..10845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10860..10865,10866..10883,10884..10890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10898..10914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10903..10925
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10922..10938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10922..10938
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10957..10977
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10959..10977
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgct gtggaatgtg tgtcagttag
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2101 tgcatctcaa ttagtcagca accatagtcc cgccgatatc cccgtgctat gccagtccta
     2161 cccgtggggg ctggggttag ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg
     2221 cccaccttgt caccatagca aaagatgctt aagttcttga tttaagggaa atctctcctc
     2281 ccagcttgtc tttctccccc tagtcaccac taacctcagg gtgaggtcag ttctccaatc
     2341 acgtgaagct ctcacgtttg ctaggtttgc agaaagggcc ttttagcttt gatcttccac
     2401 ggagcagtaa agcttgccag gccctcccca ggaattcaca tgcccttgcc atacaaggct
     2461 ttccaaacac gccaccctga ctcttcagaa aaccccaccc cagtccactt tcagctcctc
     2521 cccgctgcca gcaagcactt ttgcaaagca gaaaaggaat aggcacactc cacctacatt
     2581 gtcagtagac tacaaatccc aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg
     2641 ccggctgctg gaaaagtgtg tcactggggc acgaggcgct ccctggaatc acatggtacc
     2701 tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg cagagccctc
     2761 tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa atattactgc
     2821 tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat ttctccatca
     2881 cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc ggagctccag
     2941 attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca ctgcagagac
     3001 ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc acacacacac
     3061 tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc cttctggagg
     3121 gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg attgcgaact
     3181 gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc atcacaacct
     3241 ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga catgacgacc
     3301 ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg ctcacatggc
     3361 tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt ggccgccatc
     3421 tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca agttgccgga
     3481 gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg gttttagggc
     3541 cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt ccttgcgaca
     3601 gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg
     3661 cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc ccgccccgcc
     3721 cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct ggcttctttt
     3781 cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg gtaaccattc
     3841 tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt taaggttgcc
     3901 ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc gactcggtgg
     3961 catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc actccaccca
     4021 tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct tctcttctgt
     4081 gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca gagccgctgg
     4141 ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa ttcctaaatc
     4201 cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga gtcttatctg
     4261 tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc tgaacagaga
     4321 gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat ttaggtggtg
     4381 ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg gagtccctct
     4441 acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc acatacttcc
     4501 caccatcaac gcgtgatagc accgaggcag tgatcaagga gttcatgcgg ttcaaggtgc
     4561 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4621 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct
     4681 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcatc aagcaccccg
     4741 ccgacatccc cgactactgg aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
     4801 tgatcttcga ggacggcggc accgtgtccg tgacccagga cacctccctg gaggacggca
     4861 ccctgatcta caaggtgaag ctccgcggcg gcaacttccc tcctgacggc cccgtaatgc
     4921 agaagaggac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag gacgtcgtgc
     4981 tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact
     5041 tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgccttc aacatcgacc
     5101 gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct
     5161 ccgtggcccg ccactccacc ggcggctccg gtggctcctc tagacctcca aaaaagaaga
     5221 gaaaggtaga agaccccttg tacaagagcc atggcttccc gccggctgtg gctgctcagg
     5281 atgatggcac gctgcccatg tcctgtgccc aggagagcgg gatggaccgt caccctgcag
     5341 cctgtgctcc tgctaggatc aatgtgtgac tcgagcccgc ccggccggcc ttctgccgct
     5401 gcccccttgt acagaatcga actctgcact tctctctcct ttacgagacg aaaaggaaaa
     5461 gcaaaccaga atcttattta tattgttata aaatattcca agatgagcct ctggccccct
     5521 gagccttctt gtaaataact cttcccccac ccccaccgcc atgctcccat cctcccctat
     5581 ttaaaccact cttgctctct ccaccctcct ctggcccctc gatttgttct gttcctgtct
     5641 caaatccaat agttcacagc tgacaagttt gtacaaaaaa gcaggctttc taccgggtag
     5701 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     5761 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     5821 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     5881 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     5941 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     6001 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     6061 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     6121 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     6181 ctccgggcct ttcgacctgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     6241 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6301 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6361 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6421 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6481 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6541 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6601 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6661 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6721 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6781 atgacccgca agcccggtgc ctgaacccag ctttcttgta caaagtggtg ataatcgaat
     6841 tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7381 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg
     7441 gaattcccgc ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7501 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7561 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7621 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7681 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7741 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7801 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7921 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7981 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8041 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8101 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8161 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8221 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8281 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8341 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8401 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8461 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8521 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8581 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8641 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8701 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8761 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8821 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8881 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8941 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9001 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9061 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9121 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9181 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9241 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9301 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9361 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9421 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9481 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9541 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9601 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag aaaggcggac
    10381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10561 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10621 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10681 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10741 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10801 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10861 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10921 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    10981 caaaagctgg agctgcaagc tt
//
