LOCUS       sequence-481765-       11342 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (yellow) fluorescent reporter of circadian rhythm, 
            based on the murine Nr1d1-gene (REVERBa protein), expression 
            enhanced by SV40 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(4)-pmRevErbA-Venus-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2664..2684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2669..2998
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2849..2984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4825..4827,4828..5538)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4867..4888)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5128..5147)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5475..5496
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5548..5568
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    6003..6027
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6035..6534
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6070..6093)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6467..6486
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6545..7144
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6545..6564)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7041..7061
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7145..7169)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7187..7775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7240..7260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7658..7669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7863..8096
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8168..8289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8205..8224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8259..8278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8329..8464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8484..8503)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8485..8503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8513..8530)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8513..8529)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8522..8544)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8671..9126
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8758..8777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8968..8989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9152..9256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9257..9325,9326..10117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9475..9494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10288..10876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10777..10796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11030..11047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11164..11185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11200..11205,11206..11223,11224..11230)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11238..11254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11243..11265
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11262..11278
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11262..11278
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11297..11317
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11299..11317
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcc
     2101 ccgtgctatg ccagtcctac ccgtgggggc tggggttagg gtgaagaaga gaaaggggtt
     2161 ttatttacac tgcttgtcgc ccaccttgtc accatagcaa aagatgctta agttcttgat
     2221 ttaagggaaa tctctcctcc cagcttgtct ttctccccct agtcaccact aacctcaggg
     2281 tgaggtcagt tctccaatca cgtgaagctc tcacgtttgc taggtttgca gaaagggcct
     2341 tttagctttg atcttccacg gagcagtaaa gcttgccagg ccctccccag gaattcacat
     2401 gcccttgcca tacaaggctt tccaaacacg ccaccctgac tcttcagaaa accccacccc
     2461 agtccacttt cagctcctcc ccgctgccag caagcacttt tgcaaagcag aaaaggaata
     2521 ggcacactcc acctacattg tcagtagact acaaatccca acaatcctgg cggtgcgcag
     2581 gcgcacagat ctcaacgtgc cggctgctgg aaaagtgtgt cactggggca cgaggcgctc
     2641 cctggaatca catggtaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2881 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     2941 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3001 gctcggtacc tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg
     3061 cagagccctc tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa
     3121 atattactgc tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat
     3181 ttctccatca cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc
     3241 ggagctccag attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca
     3301 ctgcagagac ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc
     3361 acacacacac tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc
     3421 cttctggagg gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg
     3481 attgcgaact gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc
     3541 atcacaacct ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga
     3601 catgacgacc ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg
     3661 ctcacatggc tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt
     3721 ggccgccatc tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca
     3781 agttgccgga gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg
     3841 gttttagggc cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt
     3901 ccttgcgaca gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg
     3961 gtgccacgtg cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc
     4021 ccgccccgcc cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct
     4081 ggcttctttt cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg
     4141 gtaaccattc tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt
     4201 taaggttgcc ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc
     4261 gactcggtgg catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc
     4321 actccaccca tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct
     4381 tctcttctgt gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca
     4441 gagccgctgg ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa
     4501 ttcctaaatc cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga
     4561 gtcttatctg tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc
     4621 tgaacagaga gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat
     4681 ttaggtggtg ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg
     4741 gagtccctct acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc
     4801 acatacttcc caccatcaac gcgtgtgagc aagggcgagg agctgttcac cggggtggtg
     4861 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4921 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     4981 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     5041 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5101 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5161 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5221 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5281 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     5341 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5401 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     5461 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5521 atggacgagc tgtacaaatc tagacctcca aaaaagaaga gaaaggtaga agaccccttg
     5581 tacaagagcc atggcttccc gccggctgtg gctgctcagg atgatggcac gctgcccatg
     5641 tcctgtgccc aggagagcgg gatggaccgt caccctgcag cctgtgctcc tgctaggatc
     5701 aatgtgtgac tcgagcccgc ccggccggcc ttctgccgct gcccccttgt acagaatcga
     5761 actctgcact tctctctcct ttacgagacg aaaaggaaaa gcaaaccaga atcttattta
     5821 tattgttata aaatattcca agatgagcct ctggccccct gagccttctt gtaaataact
     5881 cttcccccac ccccaccgcc atgctcccat cctcccctat ttaaaccact cttgctctct
     5941 ccaccctcct ctggcccctc gatttgttct gttcctgtct caaatccaat agttcacagc
     6001 tgacaagttt gtacaaaaaa gcaggctttc taccgggtag gggaggcgct tttcccaagg
     6061 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6121 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6181 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6241 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6301 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6361 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6421 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6481 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6541 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6601 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6661 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6721 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6781 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6841 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6901 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6961 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     7021 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     7081 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7141 ctgaacccag ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga
     7201 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7261 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7321 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7381 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7441 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7501 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7561 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7621 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7681 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7741 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgcttt
     7801 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7861 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     7921 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7981 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8041 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8101 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8161 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8281 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8401 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8461 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     8521 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8581 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8641 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     8701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8821 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8881 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9061 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     9121 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    11101 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    11161 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    11221 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
    11281 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
    11341 tt
//
