LOCUS       sequence-481767-       11192 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (yellow) fluorescent reporter of circadian rhythm, 
            based on the murine Nr1d1-gene (REVERBa protein), expression 
            enhanced by synthtic JeT promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-JeT-pmRevErbA-Venus-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(4675..4677,4678..5388)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4717..4738)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4978..4997)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5325..5346
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5398..5418
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5853..5877
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5885..6384
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5920..5943)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6317..6336
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6395..6994
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6395..6414)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6891..6911
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6995..7019)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7037..7625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7090..7110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7508..7519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7713..7946
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8018..8139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8055..8074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8109..8128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8179..8314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8334..8353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8335..8353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8363..8380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8363..8379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8372..8394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8521..8976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8608..8627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8818..8839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9002..9106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9107..9175,9176..9967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9325..9344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10138..10726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10627..10646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10880..10897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11014..11035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11050..11055,11056..11073,11074..11080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11088..11104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11093..11115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11112..11128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11112..11128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11147..11167
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11149..11167
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcg
     2101 aattcgggcg gagttagggc ggagccaatc agcgtgcgcc gttccgaaag ttgcctttta
     2161 tggctgggcg gagaatgggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     2221 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatccctgtg
     2281 atcgtcactt gacagatatc cccgtgctat gccagtccta cccgtggggg ctggggttag
     2341 ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg cccaccttgt caccatagca
     2401 aaagatgctt aagttcttga tttaagggaa atctctcctc ccagcttgtc tttctccccc
     2461 tagtcaccac taacctcagg gtgaggtcag ttctccaatc acgtgaagct ctcacgtttg
     2521 ctaggtttgc agaaagggcc ttttagcttt gatcttccac ggagcagtaa agcttgccag
     2581 gccctcccca ggaattcaca tgcccttgcc atacaaggct ttccaaacac gccaccctga
     2641 ctcttcagaa aaccccaccc cagtccactt tcagctcctc cccgctgcca gcaagcactt
     2701 ttgcaaagca gaaaaggaat aggcacactc cacctacatt gtcagtagac tacaaatccc
     2761 aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg ccggctgctg gaaaagtgtg
     2821 tcactggggc acgaggcgct ccctggaatc acatggtacc tgctccagtg ccgcgtgcgg
     2881 cccgggaacc ctgggctgct ggcgcctgcg cagagccctc tgtcccaggg aaaggctcgg
     2941 gcaaaaggcg gttgagattg gcagagtgaa atattactgc tgaggaaacg tagcagggca
     3001 cacgtctctc tttgcgtcct ggtgcctcat ttctccatca cctacttact tcctggttgc
     3061 agcttctctt cctttgggac ttttgcaccc ggagctccag attcattacc ctgcttcact
     3121 gcggagcctt caggagtggc atccggtgca ctgcagagac ccgactctcc ttgctacctt
     3181 atagccagaa ctgctgcagg ctgattcttc acacacacac tctctgctct tcccatgcaa
     3241 atcagatctc aggtgcctca gcgtcctacc cttctggagg gctgcagtat agccacccca
     3301 agaccttact gctcagtgcc tggaatcctg attgcgaact gcggggctca ctcgtctccc
     3361 tcagccattg cccagggggc gagagaggcc atcacaacct ccagtttgtg tcaaggtcca
     3421 gtttgaatga ccgctttcag ctggtgaaga catgacgacc ctggactcca ataacaacac
     3481 aggtactgag attcttatct ttgctcaggg ctcacatggc tgcagagccg ggcccacgtg
     3541 ctgcatttgt tttcattcag tagaagccgt ggccgccatc tcattggtca ttgcctcccg
     3601 gcctcgttac ataatgagct ccgcccctca agttgccgga gcaacgcttg tcgccttctc
     3661 tcctcccctc agttgtcccg gcccagcttg gttttagggc cagcctacga cccaggacgc
     3721 ccgggagggg cggggcgagg gcggggtttt ccttgcgaca gaagggaagc ggagaaggcg
     3781 gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg cgaggggcac acgtggagtg
     3841 gggacgtgag gccagctgag ggccaggctc ccgccccgcc cttctccgcc tagatctggg
     3901 tgaagctggg ctgactcacc gcccccgcct ggcttctttt cctctcagaa aataacccat
     3961 cctccaaccc agccgtgaaa ccatagccgg gtaaccattc tgtgacttat tttacaggca
     4021 gggttcaccc tgaccaacct tgagctattt taaggttgcc ctgcctggtt tagtgtgtgt
     4081 cagtgggtga gcaagacatg tgcttctgtc gactcggtgg catggcatgt gtcacctcac
     4141 acacgtgtct gaggatcctc ccctttcccc actccaccca tggccggcca ggctaagtac
     4201 ttggcagagg tgtggcagac aagtggccct tctcttctgt gcataccaca ggagccgaat
     4261 cacctggccc gcaatgtaac cagagggaca gagccgctgg ctgagtggct atagagctaa
     4321 agttaccacc gggctgagaa ggaaggggaa ttcctaaatc cctagaatgg atactttgtt
     4381 tctccctttc atctcctgca actcatctga gtcttatctg tcacatttct aagtctcttg
     4441 tgactcatct ctcagaggcc agagccaggc tgaacagaga gtccttccct gcagtactct
     4501 atgagaatat gtctttgggt gttctcttat ttaggtggtg ttatcaccta cattggctct
     4561 agtggctcct ccccgagccg gaccagcccg gagtccctct acagtgacag ctccaatggc
     4621 agcttccagt ccctgactca aggttgtccc acatacttcc caccatcaac gcgtgtgagc
     4681 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4741 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4801 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4861 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4921 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4981 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5041 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5101 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     5161 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     5221 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5281 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5341 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaatc tagacctcca
     5401 aaaaagaaga gaaaggtaga agaccccttg tacaagagcc atggcttccc gccggctgtg
     5461 gctgctcagg atgatggcac gctgcccatg tcctgtgccc aggagagcgg gatggaccgt
     5521 caccctgcag cctgtgctcc tgctaggatc aatgtgtgac tcgagcccgc ccggccggcc
     5581 ttctgccgct gcccccttgt acagaatcga actctgcact tctctctcct ttacgagacg
     5641 aaaaggaaaa gcaaaccaga atcttattta tattgttata aaatattcca agatgagcct
     5701 ctggccccct gagccttctt gtaaataact cttcccccac ccccaccgcc atgctcccat
     5761 cctcccctat ttaaaccact cttgctctct ccaccctcct ctggcccctc gatttgttct
     5821 gttcctgtct caaatccaat agttcacagc tgacaagttt gtacaaaaaa gcaggctttc
     5881 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5941 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     6001 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     6061 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     6121 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     6181 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     6241 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     6301 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     6361 tcttcctcat ctccgggcct ttcgacctgc caccatgacc gagtacaagc ccacggtgcg
     6421 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6481 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6541 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6601 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6661 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6721 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6781 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6841 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6901 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6961 cacctggtgc atgacccgca agcccggtgc ctgaacccag ctttcttgta caaagtggtg
     7021 ataatcgaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7081 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7141 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7201 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7261 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7321 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7381 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7441 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7501 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7561 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7621 cccgcatcgg gaattcccgc ggttcgcttt aagaccaatg acttacaagg cagctgtaga
     7681 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7741 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7801 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7861 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7921 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7981 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     8041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     8161 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8221 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8281 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     8341 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8401 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8461 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     8521 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8581 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8641 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8701 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8761 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8821 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8881 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8941 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     9001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag
    10561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    11041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    11101 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
    11161 ctaaagggaa caaaagctgg agctgcaagc tt
//
