LOCUS       sequence-481768-       11032 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (yellow) fluorescent reporter of circadian rhythm, 
            based on the murine Nr1d1-gene (REVERBa protein), expression 
            enhanced by SV40 promoter fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1963..1983)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             join(4515..4517,4518..5228)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4557..4578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4818..4837)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5165..5186
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5238..5258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5693..5717
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5725..6224
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5760..5783)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6157..6176
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6235..6834
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6235..6254)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6731..6751
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6835..6859)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6877..7465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6930..6950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7348..7359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7553..7786
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7858..7979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7895..7914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7949..7968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8019..8154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8174..8193)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8175..8193)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8203..8220)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8203..8219)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8212..8234)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8361..8816
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8448..8467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8658..8679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8842..8946
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8947..9015,9016..9807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9165..9184)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9978..10566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10467..10486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10720..10737
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10854..10875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10890..10895,10896..10913,10914..10920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10928..10944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10933..10955
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10952..10968
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10952..10968
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10987..11007
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10989..11007
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgct gtggaatgtg tgtcagttag
     1981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2041 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2101 tgcatctcaa ttagtcagca accatagtcc cgccgatatc cccgtgctat gccagtccta
     2161 cccgtggggg ctggggttag ggtgaagaag agaaaggggt tttatttaca ctgcttgtcg
     2221 cccaccttgt caccatagca aaagatgctt aagttcttga tttaagggaa atctctcctc
     2281 ccagcttgtc tttctccccc tagtcaccac taacctcagg gtgaggtcag ttctccaatc
     2341 acgtgaagct ctcacgtttg ctaggtttgc agaaagggcc ttttagcttt gatcttccac
     2401 ggagcagtaa agcttgccag gccctcccca ggaattcaca tgcccttgcc atacaaggct
     2461 ttccaaacac gccaccctga ctcttcagaa aaccccaccc cagtccactt tcagctcctc
     2521 cccgctgcca gcaagcactt ttgcaaagca gaaaaggaat aggcacactc cacctacatt
     2581 gtcagtagac tacaaatccc aacaatcctg gcggtgcgca ggcgcacaga tctcaacgtg
     2641 ccggctgctg gaaaagtgtg tcactggggc acgaggcgct ccctggaatc acatggtacc
     2701 tgctccagtg ccgcgtgcgg cccgggaacc ctgggctgct ggcgcctgcg cagagccctc
     2761 tgtcccaggg aaaggctcgg gcaaaaggcg gttgagattg gcagagtgaa atattactgc
     2821 tgaggaaacg tagcagggca cacgtctctc tttgcgtcct ggtgcctcat ttctccatca
     2881 cctacttact tcctggttgc agcttctctt cctttgggac ttttgcaccc ggagctccag
     2941 attcattacc ctgcttcact gcggagcctt caggagtggc atccggtgca ctgcagagac
     3001 ccgactctcc ttgctacctt atagccagaa ctgctgcagg ctgattcttc acacacacac
     3061 tctctgctct tcccatgcaa atcagatctc aggtgcctca gcgtcctacc cttctggagg
     3121 gctgcagtat agccacccca agaccttact gctcagtgcc tggaatcctg attgcgaact
     3181 gcggggctca ctcgtctccc tcagccattg cccagggggc gagagaggcc atcacaacct
     3241 ccagtttgtg tcaaggtcca gtttgaatga ccgctttcag ctggtgaaga catgacgacc
     3301 ctggactcca ataacaacac aggtactgag attcttatct ttgctcaggg ctcacatggc
     3361 tgcagagccg ggcccacgtg ctgcatttgt tttcattcag tagaagccgt ggccgccatc
     3421 tcattggtca ttgcctcccg gcctcgttac ataatgagct ccgcccctca agttgccgga
     3481 gcaacgcttg tcgccttctc tcctcccctc agttgtcccg gcccagcttg gttttagggc
     3541 cagcctacga cccaggacgc ccgggagggg cggggcgagg gcggggtttt ccttgcgaca
     3601 gaagggaagc ggagaaggcg gtgcttcgag ttccggggtt cctcgttggg gtgccacgtg
     3661 cgaggggcac acgtggagtg gggacgtgag gccagctgag ggccaggctc ccgccccgcc
     3721 cttctccgcc tagatctggg tgaagctggg ctgactcacc gcccccgcct ggcttctttt
     3781 cctctcagaa aataacccat cctccaaccc agccgtgaaa ccatagccgg gtaaccattc
     3841 tgtgacttat tttacaggca gggttcaccc tgaccaacct tgagctattt taaggttgcc
     3901 ctgcctggtt tagtgtgtgt cagtgggtga gcaagacatg tgcttctgtc gactcggtgg
     3961 catggcatgt gtcacctcac acacgtgtct gaggatcctc ccctttcccc actccaccca
     4021 tggccggcca ggctaagtac ttggcagagg tgtggcagac aagtggccct tctcttctgt
     4081 gcataccaca ggagccgaat cacctggccc gcaatgtaac cagagggaca gagccgctgg
     4141 ctgagtggct atagagctaa agttaccacc gggctgagaa ggaaggggaa ttcctaaatc
     4201 cctagaatgg atactttgtt tctccctttc atctcctgca actcatctga gtcttatctg
     4261 tcacatttct aagtctcttg tgactcatct ctcagaggcc agagccaggc tgaacagaga
     4321 gtccttccct gcagtactct atgagaatat gtctttgggt gttctcttat ttaggtggtg
     4381 ttatcaccta cattggctct agtggctcct ccccgagccg gaccagcccg gagtccctct
     4441 acagtgacag ctccaatggc agcttccagt ccctgactca aggttgtccc acatacttcc
     4501 caccatcaac gcgtgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4561 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4621 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     4681 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     4741 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4801 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4861 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4921 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     4981 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     5041 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5101 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5161 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5221 tgtacaaatc tagacctcca aaaaagaaga gaaaggtaga agaccccttg tacaagagcc
     5281 atggcttccc gccggctgtg gctgctcagg atgatggcac gctgcccatg tcctgtgccc
     5341 aggagagcgg gatggaccgt caccctgcag cctgtgctcc tgctaggatc aatgtgtgac
     5401 tcgagcccgc ccggccggcc ttctgccgct gcccccttgt acagaatcga actctgcact
     5461 tctctctcct ttacgagacg aaaaggaaaa gcaaaccaga atcttattta tattgttata
     5521 aaatattcca agatgagcct ctggccccct gagccttctt gtaaataact cttcccccac
     5581 ccccaccgcc atgctcccat cctcccctat ttaaaccact cttgctctct ccaccctcct
     5641 ctggcccctc gatttgttct gttcctgtct caaatccaat agttcacagc tgacaagttt
     5701 gtacaaaaaa gcaggctttc taccgggtag gggaggcgct tttcccaagg cagtctggag
     5761 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     5821 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     5881 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     5941 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6001 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6061 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6121 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6181 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc caccatgacc
     6241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacccag
     6841 ctttcttgta caaagtggtg ataatcgaat tccgataatc aacctctgga ttacaaaatt
     6901 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6961 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7021 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7081 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7141 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7201 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7261 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7321 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7381 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7441 atctcccttt gggccgcctc cccgcatcgg gaattcccgc ggttcgcttt aagaccaatg
     7501 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7561 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     7621 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7681 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7741 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7801 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7981 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8041 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8101 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     8161 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     8221 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8281 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     8341 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     8401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8701 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8761 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     8821 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8881 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8941 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9001 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9061 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9121 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9181 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9241 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9301 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9361 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9421 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9481 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9541 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9601 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9661 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9781 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10381 gccacgcttc ccgaagagag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10621 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10681 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10741 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10801 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10861 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10921 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    10981 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc tt
//
