LOCUS       sequence-481773-       10620 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  Lentiviral expression of SNAP-tagged NF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Weiss BG, Keth JM, Bhatt K, Doyal M, Hahn KM, Noh J, Isogai T, 
            Danuser G
  TITLE     Morphological control of merlin-Rac antagonism in 
            proliferation-promoting signaling.
  JOURNAL   Sci Signal. 2025 May 20;18(887):eadk0922. doi: 
            10.1126/scisignal.adk0922. Epub 2025 May 20.
  PUBMED    40392939
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2025-05-20"; volume: "18(887):eadk0922. doi"; 
            pages: " 10.1126/scisignal.adk0922. Epub 2025 May 20"
COMMENT     Sequence Label: pLVX-IRES-NEO-NF2-18-SNAP
FEATURES             Location/Qualifiers
     source          1..10620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2561..3355)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2672..2691)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3282..3301
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    3369..3936
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3529..3548)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3758..3775
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(3943..4488)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     primer_bind     complement(6407..6431)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6428..6631)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6457..6477)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6632..6935
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6993..7108
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(7371..7415)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7600..7833
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8330..8455
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8502..9135
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9303..9437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9327..9346)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9381..9400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9485..9589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9590..9658,9659..10450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9808..9827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgt tcagaagaac tcgtcaagaa
     2581 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     2641 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     2701 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     2761 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     2821 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     2881 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     2941 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     3001 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     3061 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     3121 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     3181 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     3241 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     3301 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca gccatggttg
     3361 tggcaagctt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct
     3421 agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca
     3481 gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag
     3541 gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc
     3601 gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt
     3661 ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc
     3721 agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa
     3781 agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca
     3841 aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag
     3901 cggcttcggc cagtaacgtt aggggggggg gcgggatcct caacccagcc caggcttgcc
     3961 cagtctgtgg ccctcgtggg ccagcagcca ctctttcacg gcgagcccgc cctcgtagcc
     4021 ccccacgtcc aggtcgccct gcaccacccg gtggcagggg atcagaatgg gcacgggatt
     4081 tccgctcagg gcggttttca cggcggcggt ggcggcggga ttgccggcca gggcggccag
     4141 gtggctgtag ctgatgacct ctccgaactt caccactttc agcagtttcc acagcacctg
     4201 gcgggtaaag ctctcctgct ggaacactgg gtggtgcagg gctggcacag ggaactcctc
     4261 gatggcctca ggctggtgaa agtaggcgtt gagccaggcg gtggcctgca tcagtggctc
     4321 tggtccgccc agcacggcgg ctggggcagg cacttccacg gcgtcggcgg cagatgttcc
     4381 tttgcccagg aagatgatac ggtgcaggcc ctgttcgcac ccagacagtt ccagcttgcc
     4441 cagagggcta tccagggtgg tgcgcttcat ttcgcagtct ttgtccatac tccctgagcc
     4501 gcctgatgca gagcccccac cgctgccgct ccgaagaccg gagagctctt caaagaaggc
     4561 cactcgggac ttggcgctct gcaaggtgag ctttttaatg gtattgtgct tgctgctgcc
     4621 acccctgtcg gagttctcat tgtgcagaat atccagagct gtctccctct ctttcagttt
     4681 caaggcctcg atttctgtct tgagttcatt gagctgctcc tgcagatgct tgctcttttc
     4741 catgtattcc actttttctt tctctatctc catggaaagc cgcttcatgt cagtatcttt
     4801 gaagtcgaaa gacaggctgt caccaatgag gttgaagctt ggtatgtcag gaggcaacgg
     4861 tgctggaatt gggttcatgg gcgggtacgt gggcttggtg gcaatctcca ggagcttctg
     4921 cttggctctt cgctccgcct cgcgtgcttc ctgcaggtcc tgcttcagct gatctgcctc
     4981 tttggccctc ctctctgact cctcagccat cttcagtgcc agcacctcgg cttccagcac
     5041 cttctgctcc atcaggcgct tctcctcctc cgtgcgaatc gctgtggcct tgatgcgctg
     5101 catttcctgc tcagcctctg cggccttctg ggccagaagt tttgcctcct cctcggtgat
     5161 ctgggccttt tcagccaaca ggtcagctgt ctcctcagac cgcatcagtg cttcgttggc
     5221 cattgttgct tcttctttca tctgcagcag cctcctctcc aactcatccc tcgtgcgttc
     5281 agcctcctcc ctcatctgct tctctcgagc gaggcgctgc cgctccatct gctttctagc
     5341 cttctcctcc ctggcctggg ctttcatctg ctgaacttcc aaagaatcgg ctttccttct
     5401 cctcataaat agatcatggt tcccgataca tagctggaga atcagcttat taacacgaag
     5461 ctttgaggag ttaaacttga agacatcaat tttcttatcc agtggtttaa tagtaaactc
     5521 cttgtcactg tacgagatgt ttcggatttc attccacggg aaggagatct tgggggtcag
     5581 tctgttctca gggtcataaa tgtgaagccc cagggcatcc actccaagca gcagctctgt
     5641 gcccttttta ttccggattg caaagtagtt cacaccgtac atctccaggt cctgagctat
     5701 cttcagatat tccatttcag cttcatccct ggctcggcct cggtgctctg cgtaccaagc
     5761 agtaattctc tcctcccaca tttccggagt catctgatac agatttatta ccctttttgg
     5821 aagcaattcc tcttgggcca aaaatccccg cttgtgaaca ctggggtcgt agtcaccata
     5881 cttggcctgg acggcgtaag aagccaggag cacagaagcc tcaggagggc agtagatctt
     5941 ttcatctaaa atctgcttct ttacctgtaa gaagaataaa tgttgtgtga tctcctgaac
     6001 cagctcctct tcagcattct caggataaaa tttggccaag aagtgaaagg tgactggttc
     6061 ttcctttgaa acatcatgat ccagtacctt cttgtccatt ttgagccagg ccactgtgtc
     6121 cttgattgtg tactgcagtc caaagaacca ggtttctcgg agccccagag tccggcacac
     6181 caaatcaaag aggtccttcc ctttccactt catctcgcaa ttgaactcca tctcggcgtc
     6241 catggtgacg atcctcacgg tgaacgtctt gggttgcttc ctcttgagag agctgaagct
     6301 catgcgggaa gcgatggccc cggccatgaa ttcaccggaa atagatcctc tagtagagtc
     6361 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
     6421 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     6481 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     6541 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     6601 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     6661 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     6721 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     6781 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     6841 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     6901 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctcccaagct tatcgataaa
     6961 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
     7021 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
     7081 accccccaat cccccctttt cttttaaagg cgataccgtc gagatccgtt cactaatcga
     7141 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
     7201 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     7261 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     7321 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     7381 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     7441 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     7501 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     7561 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     7621 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     7681 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     7741 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     7801 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     7861 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     7921 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     7981 agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     8041 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     8101 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     8161 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     8221 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     8281 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     8341 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     8401 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     8461 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     8521 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     8581 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     8641 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     8701 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     8761 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     8821 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     8881 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     8941 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     9001 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     9061 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
     9121 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
     9181 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
     9241 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
     9301 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     9361 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     9421 gctgcaataa acaagttgat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
     9481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    10321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
