LOCUS       sequence-481774-       11173 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  A lentiviral (yellow) fluorescent reporter of circadian rhythm, 
            based on the murine Nr1d1-gene (REVERBa protein), expression 
            enhanced by SV40 promoter fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
  TITLE     A lentiviral fluorescent reporter to study circadian rhythms in 
            single cells
  JOURNAL   bioRxiv 2025.06.30.661926
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.30.661926"
COMMENT     Sequence Label: pL6puro-pSV40(3)-pmRevErbA-Venus-NLS-Pest
FEATURES             Location/Qualifiers
     source          1..11173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2664..2684)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             join(4656..4658,4659..5369)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4698..4719)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4959..4978)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5306..5327
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5379..5399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    5834..5858
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5866..6365
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5901..5924)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6298..6317
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6376..6975
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6376..6395)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6872..6892
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6976..7000)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7018..7606
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7071..7091)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7489..7500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7694..7927
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7999..8120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8036..8055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8090..8109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8160..8295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8315..8334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8316..8334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8344..8361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8344..8360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8353..8375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8502..8957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8589..8608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8799..8820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8983..9087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9088..9156,9157..9948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9306..9325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10119..10707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10608..10627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10861..10878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10995..11016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11031..11036,11037..11054,11055..11061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11069..11085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11074..11096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11093..11109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11093..11109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11128..11148
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11130..11148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcc ctctctctat gaggacctca
     1981 gagcactttc cctttgttct cctttttatt tcccagacaa agggaaatga ctcaccccaa
     2041 agtcacttga gtgggcagtg tggtgtcata taagcaaaca gggagtccct agggatatcc
     2101 ccgtgctatg ccagtcctac ccgtgggggc tggggttagg gtgaagaaga gaaaggggtt
     2161 ttatttacac tgcttgtcgc ccaccttgtc accatagcaa aagatgctta agttcttgat
     2221 ttaagggaaa tctctcctcc cagcttgtct ttctccccct agtcaccact aacctcaggg
     2281 tgaggtcagt tctccaatca cgtgaagctc tcacgtttgc taggtttgca gaaagggcct
     2341 tttagctttg atcttccacg gagcagtaaa gcttgccagg ccctccccag gaattcacat
     2401 gcccttgcca tacaaggctt tccaaacacg ccaccctgac tcttcagaaa accccacccc
     2461 agtccacttt cagctcctcc ccgctgccag caagcacttt tgcaaagcag aaaaggaata
     2521 ggcacactcc acctacattg tcagtagact acaaatccca acaatcctgg cggtgcgcag
     2581 gcgcacagat ctcaacgtgc cggctgctgg aaaagtgtgt cactggggca cgaggcgctc
     2641 cctggaatca catggtaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     2701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2821 aaccatagtc ccgccggtac ctgctccagt gccgcgtgcg gcccgggaac cctgggctgc
     2881 tggcgcctgc gcagagccct ctgtcccagg gaaaggctcg ggcaaaaggc ggttgagatt
     2941 ggcagagtga aatattactg ctgaggaaac gtagcagggc acacgtctct ctttgcgtcc
     3001 tggtgcctca tttctccatc acctacttac ttcctggttg cagcttctct tcctttggga
     3061 cttttgcacc cggagctcca gattcattac cctgcttcac tgcggagcct tcaggagtgg
     3121 catccggtgc actgcagaga cccgactctc cttgctacct tatagccaga actgctgcag
     3181 gctgattctt cacacacaca ctctctgctc ttcccatgca aatcagatct caggtgcctc
     3241 agcgtcctac ccttctggag ggctgcagta tagccacccc aagaccttac tgctcagtgc
     3301 ctggaatcct gattgcgaac tgcggggctc actcgtctcc ctcagccatt gcccaggggg
     3361 cgagagaggc catcacaacc tccagtttgt gtcaaggtcc agtttgaatg accgctttca
     3421 gctggtgaag acatgacgac cctggactcc aataacaaca caggtactga gattcttatc
     3481 tttgctcagg gctcacatgg ctgcagagcc gggcccacgt gctgcatttg ttttcattca
     3541 gtagaagccg tggccgccat ctcattggtc attgcctccc ggcctcgtta cataatgagc
     3601 tccgcccctc aagttgccgg agcaacgctt gtcgccttct ctcctcccct cagttgtccc
     3661 ggcccagctt ggttttaggg ccagcctacg acccaggacg cccgggaggg gcggggcgag
     3721 ggcggggttt tccttgcgac agaagggaag cggagaaggc ggtgcttcga gttccggggt
     3781 tcctcgttgg ggtgccacgt gcgaggggca cacgtggagt ggggacgtga ggccagctga
     3841 gggccaggct cccgccccgc ccttctccgc ctagatctgg gtgaagctgg gctgactcac
     3901 cgcccccgcc tggcttcttt tcctctcaga aaataaccca tcctccaacc cagccgtgaa
     3961 accatagccg ggtaaccatt ctgtgactta ttttacaggc agggttcacc ctgaccaacc
     4021 ttgagctatt ttaaggttgc cctgcctggt ttagtgtgtg tcagtgggtg agcaagacat
     4081 gtgcttctgt cgactcggtg gcatggcatg tgtcacctca cacacgtgtc tgaggatcct
     4141 cccctttccc cactccaccc atggccggcc aggctaagta cttggcagag gtgtggcaga
     4201 caagtggccc ttctcttctg tgcataccac aggagccgaa tcacctggcc cgcaatgtaa
     4261 ccagagggac agagccgctg gctgagtggc tatagagcta aagttaccac cgggctgaga
     4321 aggaagggga attcctaaat ccctagaatg gatactttgt ttctcccttt catctcctgc
     4381 aactcatctg agtcttatct gtcacatttc taagtctctt gtgactcatc tctcagaggc
     4441 cagagccagg ctgaacagag agtccttccc tgcagtactc tatgagaata tgtctttggg
     4501 tgttctctta tttaggtggt gttatcacct acattggctc tagtggctcc tccccgagcc
     4561 ggaccagccc ggagtccctc tacagtgaca gctccaatgg cagcttccag tccctgactc
     4621 aaggttgtcc cacatacttc ccaccatcaa cgcgtgtgag caagggcgag gagctgttca
     4681 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4741 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
     4801 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
     4861 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4921 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4981 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5041 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5101 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     5161 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5221 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     5281 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5341 tcactctcgg catggacgag ctgtacaaat ctagacctcc aaaaaagaag agaaaggtag
     5401 aagacccctt gtacaagagc catggcttcc cgccggctgt ggctgctcag gatgatggca
     5461 cgctgcccat gtcctgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctc
     5521 ctgctaggat caatgtgtga ctcgagcccg cccggccggc cttctgccgc tgcccccttg
     5581 tacagaatcg aactctgcac ttctctctcc tttacgagac gaaaaggaaa agcaaaccag
     5641 aatcttattt atattgttat aaaatattcc aagatgagcc tctggccccc tgagccttct
     5701 tgtaaataac tcttccccca cccccaccgc catgctccca tcctccccta tttaaaccac
     5761 tcttgctctc tccaccctcc tctggcccct cgatttgttc tgttcctgtc tcaaatccaa
     5821 tagttcacag ctgacaagtt tgtacaaaaa agcaggcttt ctaccgggta ggggaggcgc
     5881 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     5941 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     6001 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     6061 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     6121 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     6181 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     6241 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     6301 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     6361 tttcgacctg ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6421 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6481 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6541 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6601 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6661 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6721 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6781 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6841 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6901 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6961 aagcccggtg cctgaaccca gctttcttgt acaaagtggt gataatcgaa ttccgataat
     7021 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7081 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7141 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7201 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7261 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7321 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7381 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     7441 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7501 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7561 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcccg
     7621 cggttcgctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7681 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7741 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7801 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7861 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7921 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7981 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8101 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8281 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     8341 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     8401 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     8461 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     8521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8941 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9001 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9061 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9121 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9181 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9241 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9301 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     9361 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9421 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9481 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9541 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9601 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9661 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9721 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9781 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9841 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9901 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9961 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10021 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10081 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10141 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10201 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10261 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10321 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10381 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10441 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10501 cagcgtgagc tatgagaaag cgccacgctt cccgaagaga gaaaggcgga caggtatccg
    10561 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10621 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10681 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10741 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10801 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10861 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10921 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10981 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    11041 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    11101 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
    11161 gagctgcaag ctt
//
