LOCUS       sequence-481785-        6691 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  AAV transfer plasmid for Syn-mediated expression of soma-targeted 
            XCaMPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Zhou S, Zhu Q, Eom M, Fang S, Subach OM, Ran C, Alvarado JS, 
            Sunkavalli PS, Dong Y, Wang Y, Hu J, Zhang H, Wang Z, Sun X, Yang T,
            Mu Y, Yoon YG, Guo ZV, Subach FV, Piatkevich KD
  TITLE     A sensitive soma-localized red fluorescent calcium indicator for in 
            vivo imaging of neuronal populations at single-cell resolution.
  JOURNAL   PLoS Biol. 2025 Apr 29;23(4):e3003048. doi: 
            10.1371/journal.pbio.3003048. eCollection 2025 Apr.
  PUBMED    40299972
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2025-04-29"; volume: "23(4):e3003048. doi"; pages: " 
            10.1371/journal.pbio.3003048. eCollection 2025 Apr"
COMMENT     Sequence Label: pAAV-Syn-SomaXCaMPR
FEATURES             Location/Qualifiers
     source          1..6691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        349..796
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      812..821
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             827..844
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             848..880
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(884..892,893..907)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1702..1721
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(2205..2225)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    3089..3677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3142..3162)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3560..3571)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3695..3902
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3954..4094
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3954..4083
                     /label=AAV2 ITR
     rep_origin      4169..4624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4256..4275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4466..4487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4641..4660)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4760..4782
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4820..4838)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4906..5010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5011..5079,5080..5871)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5229..5248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6042..6630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6531..6550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
      361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
      421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
      481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
      541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
      601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
      661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
      721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
      781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg ggttctcatc atcatcatca
      841 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      901 cgataaggat ctcgccacca tggtcgacgt caagctcatc cccagcctgg ccactgtgat
      961 cctggtcaag tctatgctga gaaagcgttc ctttggaaac ccatttcccg tggtttccga
     1021 gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaaggggc tgaggctgaa
     1081 ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     1141 gctgcccggc gcctacatcg tggacatcaa gttggacatc gtgtcccaca acgaggacta
     1201 caccatcgtg gaacagtgcg aacgcgccga gggccgccac tccaccggcg gcatggacga
     1261 gctgtacaag ggcggtaccg gagggagcct ggtgagcaag ggcgaggagg acaacatggc
     1321 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     1381 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
     1441 tgtcgtcgtt aagggtggtc ctctgccctt cgcctgggac atcctgtccc ctcagttcat
     1501 gtacggctcc aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
     1561 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
     1621 cgttaaccag gactcctccc tgcaagacgg cgtgttcatc tacaaggtga agctgcgcgg
     1681 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggccac
     1741 ccgcgaccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga
     1801 caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg
     1861 gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga
     1921 tggcacattc gacttccctg agttcctgac aatgatggca agaaaaatga gctacagaga
     1981 caccgaagaa gaaattagag aagcgttccg tgtgtttgat aaggatggca atggctacat
     2041 cggcgcagca gagcttcgcc acgtgatgac agaccttgga gagaagttaa cagatgaaga
     2101 ggttgatgaa atgatcaggg tggcagacat cgatggggat ggtcaggtaa actacgaaga
     2161 gtttgtacaa atgatgacag cgaagggcgg agggactgga ggcagtggtg gtggtggtgg
     2221 tggtgaattc ccggtgaaac agactttgaa ttttgacctt ctcaagttgg cgggagacgt
     2281 ggagtccaac ccaggcggat ctggcggtag cggagggaca ggtggctctg gagggagcgg
     2341 aggcactggc ggatctggtg ggagcggagg tacctcaggc cggactcaga tctcaagctc
     2401 aagcttcgag ttcagcagca aagtctcacg cgacaccctg tacgaggcgg tgcgggaagt
     2461 cctgcacggg aaccagcgca agcgccgcaa gtttctggag acggtggagc tgcagatcag
     2521 cctgaagaac tacgaccctc agaaggacaa acgtttctcg ggcaccgtca ggctcaagtc
     2581 caccccacgc cccaagttct cggtgtgcgt tctgggggac cagcagcact gtgatgaagc
     2641 caaggccgtg gatatccccc acatggacat cgaggcgctc aagaaactta acaaaaacaa
     2701 gaagttggtc aagaagctgg ctaagaagta cgatgccttt ttggcctctg agtctctgat
     2761 taagcagatc ccacgtatcc tgggcccagg cctaaacaag gctggcaagt tcccctccct
     2821 gctgacacac aatgaaaaca tggtggccaa agtggatgag gtgaaatcga caatcaagtt
     2881 ccagatgaag aaggtgctgt gtttggccgt cgctgttggc cacgtgaaga tgaccgatga
     2941 tgagctagtc tacaacattc atctggctgt caatttcttg gtgtccttgc ttaagaaaaa
     3001 ctggcaaaac gtgcgggctc tgtacatcaa gagcaccatg ggcaagcccc agcgtctgta
     3061 ttaggaattc cgtacaaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3121 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3181 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3241 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3301 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3361 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3421 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3481 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     3541 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3601 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3661 ttgggccgcc tccccgcatc gataccggct cgagctgtgc cttctagttg ccagccatct
     3721 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3781 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3841 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3901 gatgcggtct cgagctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3961 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4021 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4081 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4141 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4201 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     4261 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4381 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4441 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4501 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     4561 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4621 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4681 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4741 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4801 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4861 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4921 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4981 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5041 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5101 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5161 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5221 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5281 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5341 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5401 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5461 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5521 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5581 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5641 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5701 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5761 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5821 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5881 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5941 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6001 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6061 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6661 ctggcctttt gctggccttt tgctcacatg t
//
