LOCUS       sequence-481861-        9774 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of 
            interest with mCherry. The cloning site is BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Niu X, Tang W, Liu Y, Liu Y, Mo B, Yu Y, Wei W
  TITLE     Prime editor-based high-throughput screening reveals functional 
            synonymous mutations in human cells
  JOURNAL   bioRxiv 2024.06.16.599253
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.06.16.599253"
COMMENT     Sequence Label: pLenti-U6-epegRNA-SV40-mCherry
FEATURES             Location/Qualifiers
     source          1..9774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2641..2889
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2641..2661
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2812..2831
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     2913..2932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2913..2931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2961..3085
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3514..3819)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3616..3635)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4139..4822)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     4737..4756
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4823..4925)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(5103..5227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     5414..5430
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5472..5505)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5561..5890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5741..5876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5897..6607
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6042..6060)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6310..6329
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6663..7251
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6716..6736)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7134..7145)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7254..7270)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7780..7960
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8022..8610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8102..8121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8781..9572,9573..9641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9404..9423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9642..9746)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag gtaccaaggt ctggcaggaa
     2641 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     2701 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     2761 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     2821 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2881 cgaaacaccg agagacgctg gcttatcgaa attaatacga ctcactatag ggagacccaa
     2941 gctggctagt taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3001 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3061 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
     3121 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
     3181 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     3241 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     3301 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     3361 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     3421 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     3481 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     3541 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3601 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3661 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     3721 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     3781 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     3841 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     3901 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     3961 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4021 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4081 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     4141 agtaagccgg atccacgcgg cgtttacgcc ccccctgcca ctcatcgcag tactgttgta
     4201 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     4261 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     4321 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     4381 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     4441 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     4501 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     4561 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     4621 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     4681 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     4741 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     4801 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     4861 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     4921 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     4981 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     5041 cgtcgggtga tgctgccaac ttagtcgact acaggtcact aataccatct aagtagttga
     5101 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5161 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     5221 aagtggttga tctagagggc ccgcggttcg aacgtctctg tttaagagct atgctggact
     5281 cgctaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     5341 cgagtcggtg cttttttttc cgtctctcgc ggttctatct agttacgcgt taaaccaact
     5401 agaatttttt tctcgaggtc gacggtatcg ataagctcgc ttcacgagat tccagcaggt
     5461 cgagggacct aataacttcg tatagcatac attatacgaa gttatattaa gggttccaag
     5521 cttaagcggc cgcgtggata accgtattac cgccatgcag gtgtgtcagt tagggtgtgg
     5581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5821 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5881 cttttgcaaa gctagcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     5941 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     6001 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     6061 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     6121 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     6181 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     6241 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     6301 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     6361 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     6421 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     6481 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     6541 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     6601 caagtaaaca tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg
     6661 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6721 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6781 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6841 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6901 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6961 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7021 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7081 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7141 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7201 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7261 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7321 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7381 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7441 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7501 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7561 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7621 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7681 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7741 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7801 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7861 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7921 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
     7981 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8041 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8101 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8161 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8221 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8281 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8341 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8401 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8461 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8521 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8581 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8641 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8701 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8761 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8821 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8881 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8941 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9001 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9061 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9121 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9181 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9241 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9301 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9361 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9421 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9481 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9541 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9601 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9661 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9721 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
