LOCUS       sequence-481863-        9328 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Expression of Myc-BirA*-G3 under control of copper-inducible 
            promoter. Can be used to generate stable cell lines by Hygromycin 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, 
            Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, 
            Ting AY, Carr SA, Perrimon N
  TITLE     Multi-omic mapping of Drosophila protein secretomes reveals 
            tissue-specific origins and inter-organ trafficking
  JOURNAL   bioRxiv 2025.07.09.659702; doi: 
            https://doi.org/10.1101/2025.07.09.659702
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.07.09.659702; doi: https://doi.org/10.1101/2025.07.09.659702"
COMMENT     Sequence Label: pMT-Myc-BirA*G3
FEATURES             Location/Qualifiers
     source          1..9328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(624..641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(795..1383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(875..894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1554..2345,2346..2414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2415..2519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2587..2605
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4001..4280
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             4326..5351
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4326..5351
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5714..5779
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5909..5929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6354..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6391..6410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6445..6464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6489..6915
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     protein_bind    6941..6965
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6989..7018
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7025..7981
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGKTGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8004..8028)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
       61 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
      121 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
      181 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
      241 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      301 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
      361 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc
      421 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
      481 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
      541 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
      601 ggaagtcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      961 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1021 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1081 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1141 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1201 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1261 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1321 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1381 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1441 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1501 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1561 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1621 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1681 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1741 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1801 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1861 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1921 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1981 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2041 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2101 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2161 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2221 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2281 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2341 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2401 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2521 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2581 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcacatt tgtacgaatt
     2641 ttttttttat caaaagttcg agtttttcac caatttcctc atcaaccgag caaggcaaac
     2701 ggctttgaat aatatggtgt tatatataca tatatcaaat cgctgctgac tgcgtgattg
     2761 atggccccaa gattacatat tatcgaatca ggattcagaa ggagatcaat gtcaaatgcg
     2821 gacaggaaca tgaaagacgc ctgttatgcg caattaaaaa tttgggttta attgctgtgg
     2881 aaactgttgt tggcggcatc ttaagttcct gtttaacaac atcaactact tatgtacgta
     2941 gaagcgttta agccatttgc atacagatga gaactggctt ttgtgctaat cagtcaagat
     3001 gactccgatg atgatgactc attacctgac cagttttcgc tgctttcttt tcaacaacta
     3061 cttgtatatg tattgtatcc aatagcaata cattgaattt ccatggtcta gtcacgtatt
     3121 atcatttaat tgacaccaag tcgtgttatt gttgagctat cgagttcagc tcaaacattt
     3181 cttattccca tgaataagcc ggcaaaaata tgcaatctat gaaagttaat ataagcaaac
     3241 cttactttga ctcaatacca atgcactttg tgtcgatagg ttcacgcaat tgaggcgatt
     3301 attccgataa cccaagcgat tgactgttcc cgtttcgatt ccaattgaaa tttggaaatg
     3361 tacaatagtt ttgctatatg ctgtcaagta cgctcttatc ttctctgggt tttcttcaga
     3421 gtttcgaaac gcttcttctt ttttttgttt tttttttttt ggaatctcgt attttggaag
     3481 gggctcccct ctggaatttg ttacactgtc gttatcattg cgaacaagcg gcccgaagct
     3541 atcagcgact ttaacattta caatgcactt ttttacgacc aattaaatgt acattttcct
     3601 ttcttcgccc gttgataagc gaacgcgatg tggcgcaggc aatgtgttgc tcttgcgaca
     3661 caaacgcaat caaaatggat tcaatttcgc tttttcccag tgaaacgaag aacgaaccga
     3721 ccatcatgat atgctcctct gcatgttgcg tattgaatca atgacaattt caattaagcc
     3781 gcccgttcgt catgcgtttt cgtgcgcttc gaaatgctga taacgctgct gtcctccaac
     3841 tgctttgcat gtggacacaa ttccatttat ttaattcttt tatttggatc ggttaaatta
     3901 aaaagcgcct tgttacgcat ttaacgttgt ttccggtgcg tggtggtttc atgcttctgg
     3961 gaacggcaaa tgggtttagg attgggaacc cctcatcatc tgttggaata tactattcaa
     4021 cctacaaaag taacgttaaa caacactact ttatatttga tatgaatggc cacacctttt
     4081 atgccataaa acatattgta agagaatacc actcttttta ttccttcttt ccttcttgta
     4141 cgttttttgc tgtaagtagg tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata
     4201 aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa ggaaatataa
     4261 attataaatt acaacaggtt atggacctgc agccaagctt ggcgctcgtc cgggggcaat
     4321 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     4381 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     4441 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     4501 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     4561 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     4621 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     4681 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     4741 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     4801 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     4861 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     4921 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     4981 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     5041 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     5101 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     5161 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     5221 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     5281 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     5341 caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc ctcagccagc
     5401 aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg gtggcacagt
     5461 tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg tcgcagtgat
     5521 tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca cgcactcggg
     5581 tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt gggtgcagat
     5641 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     5701 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     5761 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     5821 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     5881 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     5941 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     6001 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     6061 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     6121 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     6181 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     6241 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     6301 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6481 tctggatcaa ttcgttgcag gacaggatgt ggtgcccgat gtgactagct ctttgctgca
     6541 ggccgtccta tcctctggtt ccgataagag acccagaact ccggcccccc accgcccacc
     6601 gccaccccca tacatatgtg gtacgcaagt aagagtgcct gcgcatgccc catgtgcccc
     6661 accaagagct ttgcatccca tacaagtccc caaagtggag aaccgaacca attcttcgcg
     6721 ggcagaacaa aagcttctgc acacgtctcc actcgaattt ggagccggcc ggcgtgtgca
     6781 aaagaggtga atcgaacgaa agacccgtgt gtaaagccgc gtttccaaaa tgtataaaac
     6841 cgagagcatc tggccaatgt gcatcagttg tggtcagcag caaaatcaag tgaatcatct
     6901 cagtgcaact aaagggggga tctcgaggga tccgatatca acaagtttgt acaaaaaagc
     6961 aggctccgcg gccgccccct tcaccatgga acaaaagctt atttctgaag aggacttggc
     7021 tagcaaagac aatactgtgc ctctgaagct gatcgctctc ctggctaatg gcgagttcca
     7081 tagtggcgaa cagctgggag aaaccctggg catgtccagg gccgctatca acaagcacat
     7141 tcagactctg cgcgactggg gcgtggacgt gttcaccgtg cccggaaagg gctactctct
     7201 gcccgagcct atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag
     7261 cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga
     7321 gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg
     7381 gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa
     7441 gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct
     7501 gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga
     7561 tagaaagctg gcaggcatcc tggtggagct ggccggaaaa acaggcgatg ctgcacagat
     7621 cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca
     7681 gggctggatc acactgcagg aagcagggat taacctggac aggaatactc tggccgctat
     7741 gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata
     7801 tctgtcacgg tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg
     7861 ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga
     7921 acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa
     7981 gtaatagaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atatcactag
     8041 ttctagtcga ccatgaagat caagatcatt gccccgccag agcgcaagta ctctgtctgg
     8101 atcggtggct ccatcctggc ttcgctgtcc accttccagc agatgtggat ctccaagcag
     8161 gagtacgacg agtccggccc ctccattgtg caccgcaagt gcttctaaga aggatcgctt
     8221 gtctgggcaa gaggatcagg atcgggatgg tcttgattct gctggaggag gaggaggaga
     8281 agtcgaggaa gcagcagcga aagtgcaagt gcgagtggtg gaagtttgga gtgcagcaca
     8341 acaaaatcaa caacaacacc aactacaaga tgaaaagagc ggaaccacct gcacaccatc
     8401 atcactatca tcatcgtttt gggcgcatgt tgtgtggttc cagcgtatta atataattaa
     8461 tttattccac atgagatatg atatgatata ctatgtattt tttgtttttt ttttatttgt
     8521 aaacctttaa tataacaaga actacaaaaa atgaaaatga gcgaaaatgc atattctgcc
     8581 attccacaca cacaccaaca acacccaaca cacgcacacc cacaagctta cacacacaca
     8641 ttcgcggcat gacaaggaca tcaagataaa gaagaactta aagaagatat ttcccaaagc
     8701 gcaaaaagaa cacacacaca ttgcaaaaca caaacaacac actagcgttt tgtacaattc
     8761 gtcagcaacc ttatgtatta ttttttaatt atgatgtaat tataaacaaa gtgaaacaaa
     8821 aatatgaaaa caaaaaggaa aatcaaatct gtcttctctt tctcccgctc tcctcgctct
     8881 ctgctgctaa cctcgccctc tcctctctca tctttttgtc tgtctctctt ccacattttt
     8941 gccggccggc aaaataataa cccacacaca ctcacacttg gctgcagttt cgcgtgcgat
     9001 attcacacac attcaagcat acaagcatac atacatatgt attttttttt tatttgtaca
     9061 cttttctaat tgcatgcgta tcgattgata agtttacgcc tgaaaatgtt aattaaaatg
     9121 tgaaaatgca actgaaaaac tgatgaaatg aaacaacaac aagcgaacaa tttgctacat
     9181 gtgtattgtc taacaaccgt tactgcaacg gttgcttcgg aaaagggtga agaggaagag
     9241 ggcacgaggt cgactagagc ggccgccgac gcgaggctgg atggccttcc ccattatgat
     9301 tcttctcgct tccggcggca tcgggatg
//
