LOCUS       sequence-481866-       13632 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  A plasmid to generate a stably expressing preclonal mixture using 
            puromycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13632)
  TITLE     Photoswitchable intein for light control of covalent protein binding
            and cleavage
REFERENCE   2  (bases 1 to 13632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCSII-cp PS Intein tTA Puro
FEATURES             Location/Qualifiers
     source          1..13632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        543..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             759..939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    986..1111
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1603..1836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2021..2065
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2339..2456
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2518..2821
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2822..3025
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3060..3995
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4647..5096
                     /codon_start=1
                     /product="""positive Magnet"" variant of an N-terminally 
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
                     /label=pMag
                     /note="rapidly heterodimerizes with nMagHigh1 upon blue 
                     light irradiation to form a complex with slow switch-off 
                     kinetics"
                     /translation="HTLYAPGGYDIMGYLIQIMNRPNPQVELGPVDTSCALILCDLKQK
                     DTPIVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID
                     RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE"
     CDS             join(5184..5801,5802..6185)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(5254..5273)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             7557..7610
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7611..8210
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7611..7630)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8107..8127
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8254..8842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8307..8327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8725..8736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9361..9541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9567..9584)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9573..9797
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9843..10271
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9930..9949)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10140..10161
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10280..10300)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10285..10614
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10465..10600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10527..10546
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10662..10709
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10728..11102
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11232..11353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11269..11288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11323..11342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11402..11418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11402..11418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11415..11437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11426..11442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11450..11456,11457..11474,11475..11480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11495..11516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11633..11650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11804..12392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11884..11903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12563..13354,13355..13423))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13186..13205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13424..13528)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13603..13622)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
       61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
      121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      781 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      841 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      901 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      961 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1021 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1081 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1141 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1201 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1261 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1321 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1381 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1441 acagcaaggg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1501 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1561 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1621 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1681 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1741 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1801 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1861 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1921 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1981 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2041 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2101 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2161 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2221 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2281 cagagacaga tccattcgat tagtgaacgg atctacaaat ggcagtattc atccacaatt
     2341 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2401 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2461 tttattacag ggacagcaga aattcacttt gaattaattc aagcttcgtg aaccggtcgt
     2521 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2581 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     2641 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     2701 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     2761 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2821 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     2881 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2941 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3001 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcaccatgg
     3061 ctggagacat gagagctgcc aacctttggc caagcccgct catgatcaaa cgctctaaga
     3121 agaacagcct ggccttgtcc ctgacggccg accagatggt cagtgccttg ttggatgctg
     3181 agccccccat actctattcc gagtatgatc ctaccagacc cttcagtgaa gcttcgatga
     3241 tgggcttact gaccaacctg gcagacaggg agctggttca catgatcaac tgggcgaaga
     3301 gggtgccagg ctttgtggat ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct
     3361 ggctagagat cctgatgatt ggtctcgtct ggcgctccat ggagcaccca gtgaagctac
     3421 tgtttgctcc taacttgctc ttggacagga accagggaaa atgtgtagag ggcatggtgg
     3481 agatcttcga catgctgctg gctacatcat ctcggttccg catgatgaat ctgcagggag
     3541 aggagtttgt gtgcctcaaa tctattattt tgcttaattc tggagtgtac acatttctgt
     3601 ccagcaccct gaagtctctg gaagagaagg accatatcca ccgagtcctg gacaagatca
     3661 cagacacttt gatccacctg atggccaagg caggcctgac cctgcagcag cagcaccagc
     3721 ggctggccca gctcctcctc atcctctccc acatcaggca catgagtaac aaaggcatgg
     3781 agcatctgta cagcatgaag tgcaagaacg tggtgcccct ctatgacctg ctgctggagg
     3841 cggcggacgc ccaccgccta catgcgccca ctagccgtgg aggggcatcc gtggaggaga
     3901 cggaccaaag ccacttggcc actgcgggct ctacttcatc gcattccttg caaaagtatt
     3961 acatcacggg ggaggcagag ggtttccctg ccacagctgg gggtggtgga tcaggaggtg
     4021 gtggatcagg aggaggtggt tctggtggag gaggttctac cggtgaaatg atgctgaaaa
     4081 aaatcctgaa aatcgaggaa ctggatgaac gcggtaccgg cagcggaggt cataccttgt
     4141 atgcgccggg gggttatgac atcatgggtt acctcataca gatcatgaat aggccgaacc
     4201 cacaagtgga actcggaccc gtcgatacct cctgcgctct cattctgtgt gaccttaagc
     4261 agaaggatac ccctatcgtg tacgcctccg aggcatttct gtacatgaca gggtactcga
     4321 acgccgaagt gctgggacgg aactgccgct tcctgcaaag cccggatgga atggtgaagc
     4381 ctaagtcaac ccgcaaatac gtggactcca acactatcaa caccatgcgc aaggccattg
     4441 accgcaatgc tgaggtgcaa gtggaagtgg tgaacttcaa gaagaatgga cagcgcttcg
     4501 tcaacttcct gactatgatc cccgtgcgcg acgagaccgg cgaatatcgg tacagcatgg
     4561 ggtttcagtg tgagacagag ggcggctcag gaggcagcgg cggttctgga ggttccggtg
     4621 gcggctcagg aggtagcgga gggtctcaca ctctttacgc ccctggagga tacgacatta
     4681 tgggatattt gattcagatt atgaaccgcc caaaccctca ggtcgaactg gggcctgtgg
     4741 acacgtcatg tgccctgatc ctgtgcgatc tgaagcaaaa ggacactccg attgtctacg
     4801 cctcggaagc cttcttgtat atgaccggat acagcaatgc agaggtgctc ggtaggaact
     4861 gcagattcct gcagtccccc gacgggatgg tgaaaccaaa gtccactcgc aaatatgtgg
     4921 actcgaacac gatcaataca atgcggaagg ccatcgaccg gaacgccgag gtccaggtgg
     4981 aggtggtcaa ctttaagaag aacggccagc ggttcgtgaa ctttctcacc atgattccgg
     5041 tccgggatga aaccggagag tacagatact ccatgggatt ccagtgcgaa accgaagggt
     5101 caggatccga actgattgat attgaagtta gcggtaacca cctgttctat gccaatgata
     5161 ttctgaccca taatagctcg agctctagat tagataaaag taaagtgatt aacagcgcat
     5221 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     5281 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     5341 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     5401 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     5461 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     5521 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     5581 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     5641 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     5701 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     5761 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc cgcgcgcgta
     5821 cgaaaaacaa ttacgggtct accatcgagg gcctgctcga tctcccggac gacgacgccc
     5881 ccgaagaggc ggggctggcg gctccgcgcc tgtcctttct ccccgcggga cacacgcgca
     5941 gactgtcgac ggcccccccg accgatgtca gcctggggga cgagctccac ttagacggcg
     6001 aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac atgttggggg
     6061 acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac ggcgctctgg
     6121 atatggccga cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg
     6181 gtgggactag tggagggtca ggggctggag ggtcgggggc aggcggttcg ggagccggag
     6241 gatctggagg gggtggttcg ggtggtggtg gtagcggcta ctgcctggac ctgaagaccc
     6301 aggtgcagac cccccagggc atgaaggaga tcagcaacat ccaggtgggc gacctggtgc
     6361 tgagcaacac cggctacaac gaggtgctga acgtgttccc caagagcaag aagaagagct
     6421 acaagatcac cctggaggac ggcaaggaga tcatctgcag cgaggagcac ctgttcccca
     6481 cccagaccgg cgagatgaac atcagcggcg gcctgaagga gggcatgtgc ctgtacgtga
     6541 agggcggctc cggaggaggt agtggcggag ggagtggtgg gagtggctct ggttcaggca
     6601 gtggttctgc aggcgatatg cgggccgcta atttgtggcc ttctcctttg atgattaaga
     6661 gaagtaaaaa aaattccttg gctctgagct tgaccgctga tcaaatggtg tccgctctcc
     6721 tcgacgccga acctcctatc ttgtacagcg aatacgaccc cacacggcca tttagcgagg
     6781 cctcaatgat gggactgctt acaaatctcg ctgatagaga actcgtgcat atgattaatt
     6841 gggccaaacg ggtccccgga ttcgtcgacc tgactctgca tgaccaagtg catctcttgg
     6901 agtgcgcatg gctggaaatt ctcatgatcg gcctggtgtg gcggagcatg gaacatcctg
     6961 tcaaactgct cttcgccccc aatctgctgc tggatcggaa tcaaggcaag tgcgtggaag
     7021 gaatggtcga aatttttgat atgctcctcg ccaccagcag caggtttaga atgatgaacc
     7081 tccaaggcga agagttcgtc tgtctgaaga gcatcatcct gctgaacagc ggggtctata
     7141 ccttcctaag tagtacatta aaaagtttgg aggaaaaaga tcacattcat cgggtgctcg
     7201 ataaaattac cgatacccta attcatctca tggctaaagc cggactcaca ctccaacaac
     7261 aacatcaaag actcgctcaa ctgttactga ttctgagcca tattagacat atgagcaata
     7321 agggaatgga acacctctat tccatgaaat gtaaaaatgt cgtccctctg tacgatctcc
     7381 tcctcgaagc cgccgatgct cataggctgc acgcccctac ctccagaggc ggagccagcg
     7441 tcgaagaaac cgatcagtcc catctggcta ccgccggaag cacctccagc cacagccttc
     7501 agaaatacta tattaccggc gaagccgaag gctttcccgc taccgccgga tccggggagg
     7561 gaagaggttc cctgctcaca tgcggagacg tagaagagaa tcccggaccc atgaccgagt
     7621 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     7681 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     7741 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7801 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7861 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7921 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7981 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8041 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8101 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8161 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gcggccgctc
     8221 tagagttaac atcgagggat caagcttatc gataatcaac ctctggatta caaaatttgt
     8281 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8341 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8401 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8461 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8521 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8581 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8641 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     8701 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8761 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8821 tccctttggg ccgcctcccc gcatcgatac cgtcgagacc tagaaaaaca tggagcaatc
     8881 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     8941 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     9001 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9061 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     9121 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     9181 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     9241 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     9301 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     9361 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9421 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9481 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9541 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     9601 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     9661 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     9721 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     9781 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     9841 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9901 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9961 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    10021 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    10081 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    10141 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    10201 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    10261 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    10321 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    10381 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    10441 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10501 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    10561 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    10621 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
    10681 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
    10741 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
    10801 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
    10861 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
    10921 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
    10981 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
    11041 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
    11101 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    11161 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    11221 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11281 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11341 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
    11401 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    11461 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    11521 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    11581 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    11641 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    11701 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    11761 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    11821 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    11881 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    11941 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    12001 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    12061 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    12121 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    12181 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    12241 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    12301 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    12361 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    12421 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    12481 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    12541 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    12601 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    12661 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    12721 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    12781 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    12841 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    12901 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    12961 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    13021 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    13081 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    13141 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    13201 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    13261 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    13321 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    13381 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    13441 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    13501 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    13561 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    13621 cgcatagtta ag
//
