LOCUS       sequence-481878-       10626 bp    DNA     circular SYN 05-AUG-2025
DEFINITION  Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush 
            JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I,
            Bartel DP, Sabatini DM, Chivukula RR
  TITLE     Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing 
            in GGC repeat disorders
  JOURNAL   Science 17 July 2025, 389(6757). doi: 10.1126/science.ado2403
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science 17 
            July 2025, 389(6757). doi: 10.1126/science.ado2403"
COMMENT     Sequence Label: pLJY1 DD-FAM98B[1-330]
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      551..1139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1040..1059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1293..1310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1427..1448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1463..1468,1469..1486,1487..1493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1501..1517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1506..1528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1525..1541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1525..1541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1560..1580
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1562..1580
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1608..1834
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1835..2015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2062..2187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2680..2913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3098..3142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4105..5283
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4336..5274
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5231..5251
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5298..5307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5304..5306,5307..5627)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             5634..5675
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6693..7281
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6746..6766)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7164..7175)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    7327..7444
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7493..8003
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7922..7940
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8025..8624
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8025..8044)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8521..8541
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8752..8985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9057..9178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9094..9113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9148..9167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9218..9353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9373..9392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9374..9392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9402..9419)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9402..9418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9411..9433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9560..10015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9647..9666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9857..9878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10041..10145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10146..10214,10215..10626,1..380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10364..10383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
       61 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      241 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      301 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      361 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      661 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1321 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1381 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1441 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     1561 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     1621 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     1681 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     1741 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     1801 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     1861 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1921 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1981 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     2041 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     2101 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     2161 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     2221 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     2281 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     2341 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     2401 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2461 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2521 caagcagcag ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2581 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     2641 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     2701 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2761 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2821 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2881 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2941 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3001 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3061 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3121 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3181 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     3241 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     3301 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     3361 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgggagg tggtccctgc
     3421 agttacgcca atgataaccc ccgccagaaa aatcttagta gccttccctt tttgttttcc
     3481 gtgccccaac tcggcggatt gactcggccc cttccggaaa cacccgaatc aacttctagt
     3541 caaattattg ttcacgccgc aatgacccac ccctggcccg cgtctgtgga actgacccct
     3601 ggtgtacagg agagttcgct gctgaaagtg gtcccaaagg ggtactagtt tttaagctcc
     3661 caactccccc tcccccagcg tctggaggat tccacaccct cgcaccgcag gggcgaggaa
     3721 gtgggcggag tccggttttg gcgccagccg ctgaggctgc caagcagaaa agccaccgct
     3781 gaggagactc cggtcactgt cctcgccccg cctccccctt ccctcccctt ggggaccacc
     3841 gggcgccacg ccgcgaacgg taagtgccgc ggtcgtcggc gcctccgccc tccccctagg
     3901 gccccaattc ccagcgggcg cggcgcgcgg cccctccccc cgccgggcgc gcgcccgctg
     3961 ccccgccctt cgtggccgcc cggcgtgggc ggtgccaccc ctccccccgg cggccccgcg
     4021 cgcagctccc ggctccctcc cccttcggat gtggcttgag ctgtaggcgc ggagacgcgt
     4081 taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4141 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg
     4201 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4261 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4321 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     4381 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     4441 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     4501 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     4561 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     4621 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     4681 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     4741 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     4801 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     4861 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     4921 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     4981 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     5041 cgggcgccgt ccaggcacct cgattagttc tcgcgctttt ggagtacgtc gtctttaggt
     5101 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     5161 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     5221 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     5281 tgaaccggtt taattaagcc accatgggag tgcaggtgga aaccatctcc ccaggagacg
     5341 ggcgcacctt ccccaagcgc ggccagacct gtgtggtgca ctacaccggg atgcttgaag
     5401 atggaaagaa agtcgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca
     5461 agcaggaggt gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag
     5521 ccaaactgac tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc
     5581 caccacatgc cactctcgtc ttcgatgtgg agcttctaaa accggaaggc tctggtaagc
     5641 ctatccctaa ccctctcctc ggtctcgatt ctacgggtgg aggtgggtcg acgatgagag
     5701 ggccggagcc gggtccccaa ccgacgatgg agggagacgt gctggacaca ctggaggcgc
     5761 tggggtataa aggaccattg ttagaagagc aagcccttac aaaggcggca gagggtggat
     5821 tatcttcacc tgaattttca gagctctgta tttggttagg ctctcaaata aaatcattat
     5881 gcaacttgga agaaagtatc acgtctgctg gaagagatga tctagagagc ttccagcttg
     5941 agataagtgg ctttttaaaa gaaatggcat gtccatattc tgtactcata tcaggagata
     6001 ttaaagatcg tttaaaaaag aaggaggact gtttgaaact tctattattt ttaagtacag
     6061 aacttcaagc ttcacagata ttacagaaca agaaacataa aaattctcaa ttagataaaa
     6121 atagtgaagt ttatcaggaa gttcaagcta tgtttgatac acttggtata cccaagtcaa
     6181 caacttctga cattccgcat atgctaaacc aagtggaatc aaaggtgaaa gatattctct
     6241 caaaggtcca gaaaaatcat gtgggaaaac cactgctgaa aatggattta aattcagaac
     6301 aggcggaaca actggaaaga atcaatgatg ctctttcctg tgaatatgag tgtcgccgac
     6361 gaatgttaat gaaacgatta gatgtgactg tacagtcctt tggatggtct gatagagcaa
     6421 aggtaaaaac agatgatata gcaagaattt atcagcctaa gcgttatgct ttgtcaccca
     6481 agacaacgat tacaatggca catctacttg ctgctcgtga agatctatcc aagatcatta
     6541 ggacaagtag tggcaccagc cgggagaaga ccgcatgtgc cattaataag gtgctgatgg
     6601 gaagggtgcc tgacagggga ggccggccga atgaaattga accaccacct ccagaaatgc
     6661 ccccttggca aaagagacaa gaatgagaat tcaatcaacc tctggattac aaaatttgtg
     6721 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6781 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6841 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6901 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6961 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7021 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7081 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7141 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7201 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7261 ccctttgggc cgcctccccg catcggctag ctcgacctcg agacaaatgg cagtattcat
     7321 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     7381 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     7441 ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg
     7501 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     7561 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     7621 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     7681 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     7741 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     7801 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     7861 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     7921 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     7981 gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc
     8041 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8101 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8161 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8221 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8281 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8341 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8401 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8461 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8521 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8581 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     8641 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga
     8701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8761 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9001 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9181 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9241 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9301 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9361 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9421 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9481 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9541 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9601 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9661 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9721 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9781 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9841 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9901 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9961 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    10021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10621 gccttg
//
