LOCUS       sequence-481880-        8454 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush 
            JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I,
            Bartel DP, Sabatini DM, Chivukula RR
  TITLE     Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing 
            in GGC repeat disorders
  JOURNAL   Science 17 July 2025, 389(6757). doi: 10.1126/science.ado2403
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science 17 
            July 2025, 389(6757). doi: 10.1126/science.ado2403"
COMMENT     Sequence Label: pDOXOFF 15xGly
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      40..175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        175..246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(247..315,316..1107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(465..484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1278..1866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1767..1786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2020..2037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2154..2175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2190..2195,2196..2213,2214..2220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2228..2244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2233..2255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2252..2268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2252..2268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2287..2307
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2289..2307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2335..2561
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2562..2742
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2789..2914
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3407..3640
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3825..3869
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4082..4199
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4268..4646
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4276..4294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4312..4330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4348..4366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4384..4402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4420..4438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4456..4474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4492..4510
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4555..4579
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4690..4699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4789..4791,4792..5502)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4789..4791,4792..5502)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4831..4852)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5092..5111)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5439..5460
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5515..5541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5554..5580
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5593..5619
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5632..6142
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6061..6079
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6152..6544
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6554..6607
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6608..7225,7226..7231,7232..7267,7268..7270,7271..7306,
                     7307..7309,7310..7345,7346..7354)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7394..7414)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7812..7823)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8028..8261
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8333..8454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8370..8389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8424..8443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
       61 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca
      181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      241 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      301 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      361 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      421 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      481 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      541 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      601 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      661 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      721 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      781 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      841 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      901 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      961 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1021 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1081 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1141 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1201 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1261 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1321 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1381 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1441 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1501 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1561 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1621 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1681 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1741 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1801 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1861 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1921 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1981 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2041 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2101 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2161 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2221 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2281 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     2341 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     2401 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     2461 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     2521 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     2581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2761 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2821 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2881 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2941 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     3001 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     3061 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     3121 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     3181 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     3241 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3301 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3361 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3421 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3481 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3541 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3601 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3661 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3721 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3781 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3841 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3901 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3961 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     4021 acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta ttcatccaca
     4081 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     4141 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     4201 gggtttatta cagggacagc agagatccac tttggccgcg aatcgggatt cgcccttaag
     4261 ctagctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
     4321 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
     4381 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
     4441 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
     4501 gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
     4561 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
     4621 tccgtaccac ttcctaccct cgtaaagtca gttggccagg cctagttggc caggcctagt
     4681 gccgaattcg ccaccatggg gttgccgctg ccagggggcg tgcggcagcg cggcggcggc
     4741 ggcggcggcg gcggcggcgg cggcggcggc ggaggctggg cctcgacggt gagcaagggc
     4801 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4861 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4921 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4981 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     5041 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5101 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5161 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5221 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5281 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5341 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5401 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5461 accgccgccg ggatcactct cggcatggac gagctgtaca agggagggag cggctatccc
     5521 tatgacgtgc ctgattacgc cggcacagga tcatacccct atgatgtgcc tgactacgct
     5581 ggcagcgccg gataccctta tgatgtgcct gattatgctt agtgaggatc cggggttggg
     5641 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     5701 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     5761 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     5821 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     5881 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     5941 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     6001 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     6061 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     6121 aatcaccgac ctctctcccc agcaattcac catggttcct ttgtctcaag aagaatccac
     6181 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     6241 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     6301 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     6361 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     6421 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     6481 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     6541 gggccccggt gccgaaggta gaggttctct cctcacttgt ggtgatgttg aagaaaaccc
     6601 tggtccaatg tctagactgg acaagagcaa agtcataaac tctgctctgg aattactcaa
     6661 tgaagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     6721 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tggcaatcga
     6781 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     6841 tctgcggaac aacgccaagt cattccgctg tgctctcctc tcacatcgcg acggggctaa
     6901 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     6961 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     7021 ccactttaca ctgggctgcg tattggagga tcaggagcat caagtagcaa aagaggaaag
     7081 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     7141 ccatcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     7201 gaaacagcta aagtgcgaaa gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga
     7261 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     7321 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc
     7381 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7441 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7501 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7561 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7621 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7681 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7741 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7801 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7861 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7921 cctccccgcc tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta
     7981 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8041 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8101 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8161 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8221 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8281 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     8341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat ctta
//
