LOCUS       sequence-481886-        8821 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Expression of human beta-catenin ∆N terminal IDR (deletion of a.a. 
            2-133) using piggyBac transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, 
            Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, 
            Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, 
            Cermakova K, Hodges HC
  TITLE     beta-catenin functions as a molecular adapter for disordered cBAF 
            interactions.
  JOURNAL   Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026.
  PUBMED    40695292
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2025 Jul 15:S1097-2765(25)00576-3. doi: 
            10.1016/j.molcel.2025.06.026."
COMMENT     Sequence Label: pPB-CAG-CTNNB1-deltaN-HA
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     343..362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(581..685)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(711..1166)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(848..869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1060..1079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1293..1315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1307..1324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1308..1324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1334..1353
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1334..1352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1382..1416
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1737..2116
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2118..2393
                     /label=chicken beta-actin promoter
     primer_bind     3334..3356
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5409..5498
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    5593..6142
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5737..5754)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5964..5983
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6613..6630)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6619..6843
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6890..6906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6914..6930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6938..6944,6945..6962,6963..6968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6983..7004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7238..7300
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7341..7361)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7341..7359)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7380..7396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7404..7420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7428..7434,7435..7452,7453..7458))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7473..7494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7611..7628)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7782..8370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7862..7881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8541..8821,1..511,512..580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
       61 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
      121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
      181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
      241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
      301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
      361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
      421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
      481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
      541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
      601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
      661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
      721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
      781 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     1021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     1081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     1141 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     1201 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1261 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1321 cagtgagcgc gcgtaatacg actcactata gggcgaattg gggcgcgcca ttctagatta
     1381 accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat tgctctctct
     1441 ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc gcttggagct
     1501 cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat aacgaccgcg
     1561 tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac tcatacgata
     1621 attatattgt tatttcatgt tctacttacg tgataactta ttatatatat attttcttgt
     1681 tatagatatc aactagaatg ctagactcga gaagcttgat cggccacgcg gatctcgaca
     1741 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1801 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1861 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1921 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1981 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2041 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2101 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2161 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     2221 ggcggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     2281 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     2341 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     2401 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     2461 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     2521 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     2581 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2641 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     2701 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     2761 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     2821 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     2881 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     2941 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     3001 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     3061 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     3121 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     3181 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     3241 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     3301 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     3361 gctctagccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     3421 tccccagggg gatccaccgg gctagcgcca ccatgcatgc agttgtaaac ttgattaact
     3481 atcaagatga tgcagaactt gccacacgtg caatccctga actgacaaaa ctgctaaatg
     3541 acgaggacca ggtggtggtt aataaggctg cagttatggt ccatcagctt tctaaaaagg
     3601 aagcttccag acacgctatc atgcgttctc ctcagatggt gtctgctatt gtacgtacca
     3661 tgcagaatac aaatgatgta gaaacagctc gttgtaccgc tgggaccttg cataaccttt
     3721 cccatcatcg tgagggctta ctggccatct ttaagtctgg aggcattcct gccctggtga
     3781 aaatgcttgg ttcaccagtg gattctgtgt tgttttatgc cattacaact ctccacaacc
     3841 ttttattaca tcaagaagga gctaaaatgg cagtgcgttt agctggtggg ctgcagaaaa
     3901 tggttgcctt gctcaacaaa acaaatgtta aattcttggc tattacgaca gactgccttc
     3961 aaattttagc ttatggcaac caagaaagca agctcatcat actggctagt ggtggacccc
     4021 aagctttagt aaatataatg aggacctata cttacgaaaa actactgtgg accacaagca
     4081 gagtgctgaa ggtgctatct gtctgctcta gtaataagcc ggctattgta gaagctggtg
     4141 gaatgcaagc tttaggactt cacctgacag atccaagtca acgtcttgtt cagaactgtc
     4201 tttggactct caggaatctt tcagatgctg caactaaaca ggaagggatg gaaggtctcc
     4261 ttgggactct tgttcagctt ctgggttcag atgatataaa tgtggtcacc tgtgcagctg
     4321 gaattctttc taacctcact tgcaataatt ataagaacaa gatgatggtc tgccaagtgg
     4381 gtggtataga ggctcttgtg cgtactgtcc ttcgggctgg tgacagggaa gacatcactg
     4441 agcctgccat ctgtgctctt cgtcatctga ccagccgaca ccaagaagca gagatggccc
     4501 agaatgcagt tcgccttcac tatggactac cagttgtggt taagctctta cacccaccat
     4561 cccactggcc tctgataaag gctactgttg gattgattcg aaatcttgcc ctttgtcccg
     4621 caaatcatgc acctttgcgt gagcagggtg ccattccacg actagttcag ttgcttgttc
     4681 gtgcacatca ggatacccag cgccgtacgt ccatgggtgg gacacagcag caatttgtgg
     4741 agggggtccg catggaagaa atagttgaag gttgtaccgg agcccttcac atcctagctc
     4801 gggatgttca caaccgaatt gttatcagag gactaaatac cattccattg tttgtgcagc
     4861 tgctttattc tcccattgaa aacatccaaa gagtagctgc aggggtcctc tgtgaacttg
     4921 ctcaggacaa ggaagctgca gaagctattg aagctgaggg agccacagct cctctgacag
     4981 agttacttca ctctaggaat gaaggtgtgg cgacatatgc agctgctgtt ttgttccgaa
     5041 tgtctgagga caagccacaa gattacaaga aacggctttc agttgagctg accagctctc
     5101 tcttcagaac agagccaatg gcttggaatg agactgctga tcttggactt gatattggtg
     5161 cccagggaga accccttgga tatcgccagg atgatcctag ctatcgttct tttcactctg
     5221 gtggatatgg ccaggatgcc ttgggtatgg accccatgat ggaacatgag atgggtggcc
     5281 accaccctgg tgctgactat ccagttgatg ggctgccaga tctggggcat gcccaggacc
     5341 tcatggatgg gctgcctcca ggtgacagca atcagctggc ctggtttgat actgacctga
     5401 ccggtatgta cccatacgat gttccagatt acgctggcta tccctatgac gtcccggact
     5461 atgcaggctc ctatccatat gacgttccag attacgcttg aacgcgttag tgcggatcct
     5521 agtgcgaatt ctagtgcgtc gactctagca acggtttccc tctagcggga tcaattccgc
     5581 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     5641 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     5701 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     5761 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     5821 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     5881 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     5941 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     6001 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     6061 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     6121 tcctttgaaa aacacgataa taccatggtc atgaaaacat ttaacatttc tcaacaagat
     6181 ctagaattag tagaagtagc gacagagaag attacaatgc tttatgagga taataaacat
     6241 catgtgggag cggcaattcg tacgaaaaca ggagaaatca tttcggcagt acatattgaa
     6301 gcgtatatag gacgagtaac tgtttgtgca gaagccattg cgattggtag tgcagtttcg
     6361 aatggacaaa aggattttga cacgattgta gctgttagac acccttattc tgacgaagta
     6421 gatagaagta ttcgagtggt aagtccttgt ggtatgtgta gggagttgat ttcagactat
     6481 gcaccagatt gttttgtgtt aatagaaatg aatggcaagt tagtcaaaac tacgattgaa
     6541 gaactcattc cactcaaata tacccgaaat taaaagtcga gatgcatcga tgatctagag
     6601 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6661 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6721 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6781 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6841 tggcttctga ggcggaaaga acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     6901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7021 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat cttaaaagtt ttgttacttt
     7081 atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt
     7141 ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa
     7201 ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat
     7261 tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagt atcgtagatt
     7321 aattaatcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     7381 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7441 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7501 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7561 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7621 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7681 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7741 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7801 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7861 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7921 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7981 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8041 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8101 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8161 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8221 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8281 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8341 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8401 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8461 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8521 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8581 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8641 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8701 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8761 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8821 c
//
